{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T15:54:12Z","timestamp":1793116452622,"version":"4.5.0"},"reference-count":39,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2014,11,17]],"date-time":"2014-11-17T00:00:00Z","timestamp":1416182400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Non-alcoholic fatty liver disease (NAFLD) is a spectrum of metabolic abnormalities ranging from simple triglyceride accumulation in the hepatocytes to hepatic steatosis with inflammation, ballooning and fibrosis. It has been demonstrated that the pathogenesis  of NAFLD involves increased oxidative stress, with consumption of the major cellular antioxidant, glutathione (GSH). Liver has a fundamental role in sulfur compound metabolism, although the data reported on plasma thiols status in NAFLD are conflicting. We recruited 63 NAFLD patients, and we analyzed all plasma thiols, such as homocysteine (Hcy), cysteine (Cys), cysteinylglycine (CysGly) and GSH, by high-performance liquid chromatography (HPLC) with fluorescence detection. Hcy, Cys and CysGly plasma levels increased in NAFLD patients (p &lt; 0.0001); whereas GSH levels were decreased in NAFLD patients when compared to controls (p &lt; 0.0001). On the contrary, patients with steatohepatitis exhibited lower levels of Hcy and Cys than subjects without. Furthermore, a positive correlation was found between Hcy and Cys and the presence of fibrosis in children with NAFLD. Taken together, these data demonstrated a defective hepatic sulfur metabolism in children with NAFLD, and that high levels of Hcy and Cys probably correlates with a pattern of more severe histological liver damage, due to mechanisms that require further studies.<\/jats:p>","DOI":"10.3390\/ijms151121202","type":"journal-article","created":{"date-parts":[[2014,11,17]],"date-time":"2014-11-17T10:19:13Z","timestamp":1416219553000},"page":"21202-21214","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":116,"title":["Plasma Levels of Homocysteine and Cysteine Increased in Pediatric NAFLD and Strongly Correlated with Severity of  Liver Damage"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0990-9419","authenticated-orcid":false,"given":"Anna","family":"Pastore","sequence":"first","affiliation":[{"name":"Metabolomics and Proteomics Unit, \"Bambino Ges\u00f9\" Children's Hospital, IRCCS, Rome 00165, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7241-6329","authenticated-orcid":false,"given":"Anna","family":"Alisi","sequence":"additional","affiliation":[{"name":"Liver Research Unit, \"Bambino Ges\u00f9\" Children's Hospital, IRCCS, Rome 00165, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gianna","family":"Di Giovamberardino","sequence":"additional","affiliation":[{"name":"Metabolomics and Proteomics Unit, \"Bambino Ges\u00f9\" Children's Hospital, IRCCS, Rome 00165, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annalisa","family":"Crudele","sequence":"additional","affiliation":[{"name":"Liver Research Unit, \"Bambino Ges\u00f9\" Children's Hospital, IRCCS, Rome 00165, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara","family":"Ceccarelli","sequence":"additional","affiliation":[{"name":"Liver Research Unit, \"Bambino Ges\u00f9\" Children's Hospital, IRCCS, Rome 00165, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nadia","family":"Panera","sequence":"additional","affiliation":[{"name":"Liver Research Unit, \"Bambino Ges\u00f9\" Children's Hospital, IRCCS, Rome 00165, Italy"},{"name":"Hepato-Metabolic Disease Unit, \"Bambino Ges\u00f9\" Children's Hospital, IRCCS, Rome 00165, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlo","family":"Dionisi-Vici","sequence":"additional","affiliation":[{"name":"Metabolomics and Proteomics Unit, \"Bambino Ges\u00f9\" Children's Hospital, IRCCS, Rome 00165, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valerio","family":"Nobili","sequence":"additional","affiliation":[{"name":"Liver Research Unit, \"Bambino Ges\u00f9\" Children's Hospital, IRCCS, Rome 00165, Italy"},{"name":"Hepato-Metabolic Disease Unit, \"Bambino Ges\u00f9\" Children's Hospital, IRCCS, Rome 00165, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2014,11,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1055\/s-0032-1306421","article-title":"Nonalcoholic fatty liver disease: Pathologic patterns and biopsy evaluation in clinical research","volume":"32","author":"Kleiner","year":"2012","journal-title":"Semin. 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