{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T03:24:49Z","timestamp":1785381889084,"version":"3.55.0"},"reference-count":48,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2014,12,29]],"date-time":"2014-12-29T00:00:00Z","timestamp":1419811200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>O. basilicum leaves produce essential oils (LEO) rich in monoterpenes. The short half-life and water insolubility are limitations for LEO medical uses. \u03b2-Cyclodextrin (\u03b2-CD) has been employed to improve the pharmacological properties of LEO. We assessed the antihyperalgesic profile of LEO, isolated or complexed in \u03b2-CD (LEO\/\u03b2-CD), on an animal model for fibromyalgia. Behavioral tests: mice were treated every day with either LEO\/\u03b2-CD (25, 50 or 100 mg\/kg, p.o.), LEO (25 mg\/kg, p.o.), tramadol (TRM  4 mg\/kg, i.p.) or vehicle (saline), and 60 min after treatment behavioral parameters were assessed. Therefore, mice were evaluated for mechanical hyperalgesia (von Frey), motor coordination (Rota-rod) and muscle strength (Grip Strength Metter) in a mice fibromyalgia model. After 27 days, we evaluated the central nervous system (CNS) pathways involved in the effect induced by experimental drugs through immunofluorescence protocol to Fos protein. The differential scanning analysis (DSC), thermogravimetry\/derivate thermogravimetry (TG\/DTG) and infrared absorption spectroscopy (FTIR) curves indicated that the products prepared were able to incorporate the LEO efficiently. Oral treatment with LEO or LEO-\u03b2CD, at all doses tested, produced a significant reduction of mechanical hyperalgesia and we were able to significantly increase Fos protein expression. Together, our results provide evidence that LEO, isolated or complexed with \u03b2-CD, produces analgesic effects on chronic  non-inflammatory pain as fibromyalgia.<\/jats:p>","DOI":"10.3390\/ijms16010547","type":"journal-article","created":{"date-parts":[[2014,12,29]],"date-time":"2014-12-29T10:58:34Z","timestamp":1419850714000},"page":"547-563","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":44,"title":["Cyclodextrin-Complexed Ocimum basilicum Leaves Essential Oil Increases Fos Protein Expression in the Central Nervous System and Produce an Antihyperalgesic Effect in Animal Models for Fibromyalgia"],"prefix":"10.3390","volume":"16","author":[{"given":"Simone","family":"Nascimento","sequence":"first","affiliation":[{"name":"Laboratory of Pre-Clinical Pharmacology (LAPEC), Department of Physiology,  Federal University of Sergipe, Av. Tancredo Neves, S\/N, Rosa Elza, CEP: 49.000-100,  S\u00e3o Crist\u00f3v\u00e3o, Sergipe 49.100-000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adriano","family":"Ara\u00fajo","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Federal University of Sergipe, S\u00e3o Crist\u00f3v\u00e3o, Sergipe 49.100-000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renan","family":"Brito","sequence":"additional","affiliation":[{"name":"Laboratory of Pre-Clinical Pharmacology (LAPEC), Department of Physiology,  Federal University of Sergipe, Av. Tancredo Neves, S\/N, Rosa Elza, CEP: 49.000-100,  S\u00e3o Crist\u00f3v\u00e3o, Sergipe 49.100-000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mairim","family":"Serafini","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Federal University of Sergipe, S\u00e3o Crist\u00f3v\u00e3o, Sergipe 49.100-000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paula","family":"Menezes","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Federal University of Sergipe, S\u00e3o Crist\u00f3v\u00e3o, Sergipe 49.100-000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Josimari","family":"DeSantana","sequence":"additional","affiliation":[{"name":"Department of Physical Therapy, Federal University of Sergipe, Aracaju, Sergipe 49.060-108, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Waldecy","family":"J\u00fanior","sequence":"additional","affiliation":[{"name":"Department of Morphology, Federal University of Sergipe, S\u00e3o Crist\u00f3v\u00e3o, Sergipe 49.100-000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pericles","family":"Alves","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Federal University of Sergipe, S\u00e3o Crist\u00f3v\u00e3o, Sergipe 49.100-000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2888-2239","authenticated-orcid":false,"given":"Arie","family":"Blank","sequence":"additional","affiliation":[{"name":"Department of Agronomic Engineering, Federal University of Sergipe, S\u00e3o Crist\u00f3v\u00e3o,  Sergipe 49.100-000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rita","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Medicinal Plants Research Center, Federal University of Piau\u00ed, Teresina, Piau\u00ed 64.049-550, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aldeidia","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Medicinal Plants Research Center, Federal University of Piau\u00ed, Teresina, Piau\u00ed 64.049-550, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ricardo","family":"Albuquerque-J\u00fanior","sequence":"additional","affiliation":[{"name":"Institute of Technology and Research, University Tiradentes, Aracaju, Sergipe 49.032-490, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jackson","family":"Almeida","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Federal University of San Francisco Valley, Petrolina,  Pernambuco 56.304-917, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5155-938X","authenticated-orcid":false,"given":"Lucindo","family":"Quintans-J\u00fanior","sequence":"additional","affiliation":[{"name":"Laboratory of Pre-Clinical Pharmacology (LAPEC), Department of Physiology,  Federal University of Sergipe, Av. Tancredo Neves, S\/N, Rosa Elza, CEP: 49.000-100,  S\u00e3o Crist\u00f3v\u00e3o, Sergipe 49.100-000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2014,12,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"117","DOI":"10.2307\/3578431","article-title":"Is radiation all bad? The search for adaptation","volume":"131","author":"Wolff","year":"1992","journal-title":"Radiat. Res."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1007\/s11926-001-0010-9","article-title":"Complementary and alternative therapies for fibromyalgia","volume":"3","author":"Crofford","year":"2001","journal-title":"Curr. Rheumatol. 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