{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T10:57:10Z","timestamp":1787655430782,"version":"build-2736575974"},"reference-count":32,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2015,3,25]],"date-time":"2015-03-25T00:00:00Z","timestamp":1427241600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["91132728"],"award-info":[{"award-number":["91132728"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Key Laboratory of Mental Health, Institute of Psychology"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Objective: The link between alterations in circadian rhythms and depression are well established, but the underlying mechanisms are far less elucidated. We investigated the circadian characteristics of immobility behavior in wild type (WT) mice and mice with mutations in core Clock genes. Methods: All mice were tested with forced swim test (FST) at 4 h intervals. Results: These experiments revealed significant diurnal rhythms associated with immobility behavior in both male and female WT mice with sex-different circadian properties. In addition, male mice showed significantly less immobility during the night phase in comparison to female mice. Female Per1Brdm1 mice also showed significant rhythmicity. However, the timing of rhythmicity was very different from that observed in female wild type mice. Male Per1Brdm1 mice showed a pattern of rhythmicity similar to  that of wild type mice. Furthermore, female Per1Brdm1 mice showed higher duration of immobility in comparison to male Per1Brdm1 mice in both daytime and early night phases. Neither Per2Brdm1 nor Clock\u039419 mice showed significant rhythmicity, but both female Per2Brdm1 and Clock\u039419 mice had lower levels of immobility, compared to males. Conclusions: This study highlights the differences in the circadian characteristics of immobility induced by FST in WT, Clock\u039419, Per1, and Per2 deficient mice.<\/jats:p>","DOI":"10.3390\/ijms16046831","type":"journal-article","created":{"date-parts":[[2015,3,25]],"date-time":"2015-03-25T11:53:17Z","timestamp":1427284397000},"page":"6831-6841","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Sex-Specific Diurnal Immobility Induced by Forced Swim Test in Wild Type and Clock Gene Deficient Mice"],"prefix":"10.3390","volume":"16","author":[{"given":"Ningyue","family":"Li","sequence":"first","affiliation":[{"name":"Key Lab of Mental Health, Institute of Psychology, Chinese Academy of Sciences, Beijing 100101, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanhua","family":"Xu","sequence":"additional","affiliation":[{"name":"Key Lab of Mental Health, Institute of Psychology, Chinese Academy of Sciences, Beijing 100101, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaojuan","family":"Chen","sequence":"additional","affiliation":[{"name":"Key Lab of Mental Health, Institute of Psychology, Chinese Academy of Sciences, Beijing 100101, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qing","family":"Duan","sequence":"additional","affiliation":[{"name":"Key Lab of Mental Health, Institute of Psychology, Chinese Academy of Sciences, Beijing 100101, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mei","family":"Zhao","sequence":"additional","affiliation":[{"name":"Key Lab of Mental Health, Institute of Psychology, Chinese Academy of Sciences, Beijing 100101, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2015,3,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"242","DOI":"10.1016\/j.biopsych.2013.02.019","article-title":"How might circadian rhythms control mood? Let me count the ways","volume":"74","author":"McClung","year":"2013","journal-title":"Biol. Psychiatry"},{"key":"ref_2","first-page":"89","article-title":"Mapping of circaseptan and circadian changes in mood","volume":"78","author":"Cornelissen","year":"2005","journal-title":"Scr. Med."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"236","DOI":"10.1016\/j.biopsych.2013.05.037","article-title":"Much ado about\u2026a moody clock","volume":"74","author":"Benedetti","year":"2013","journal-title":"Biol. Psychiatry"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1177\/0748730412456367","article-title":"Cellular circadian clocks in mood disorders","volume":"27","author":"McCarthy","year":"2012","journal-title":"J. Biol. Rhythm."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"713","DOI":"10.1146\/annurev.neuro.23.1.713","article-title":"Molecular genetics of circadian rhythms in mammals","volume":"23","author":"King","year":"2000","journal-title":"Annu. Rev. Neurosci."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"344","DOI":"10.1037\/a0036029","article-title":"The role of the circadian clock in animal models of mood disorders","volume":"128","author":"Landgraf","year":"2014","journal-title":"Behav. Neurosci."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"734","DOI":"10.1038\/sj.npp.1300121","article-title":"Circadian clock-related polymorphisms in seasonal affective disorder and their relevance to diurnal preference","volume":"28","author":"Johansson","year":"2003","journal-title":"Neuropsychopharmacology"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1080\/07853890701278795","article-title":"Three circadian clock genes Per2, Arntl, and Npas2 contribute to winter depression","volume":"39","author":"Partonen","year":"2007","journal-title":"Ann. Med."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"6406","DOI":"10.1073\/pnas.0609625104","article-title":"Mania-like behavior induced by disruption of CLOCK","volume":"104","author":"Roybal","year":"2007","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"678","DOI":"10.1016\/j.cub.2008.04.012","article-title":"Regulation of monoamine oxidase a by circadian-clock components implies clock influence on mood","volume":"18","author":"Hampp","year":"2008","journal-title":"Curr. Biol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"242","DOI":"10.1111\/ejn.12010","article-title":"Circadian genes Period 1 and Period 2 in the nucleus accumbens regulate anxiety-related behavior","volume":"37","author":"Spencer","year":"2013","journal-title":"Eur. J. Neurosci."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1016\/j.physbeh.2013.05.012","article-title":"Factors influencing behavior in the forced swim test","volume":"118","author":"Bogdanova","year":"2013","journal-title":"Physiol. Behav."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1186\/1479-7364-8-4","article-title":"Forced swim test induces divergent global transcriptomic alterations in the hippocampus of high vs. low novelty-seeker rats","volume":"8","author":"Pitychoutis","year":"2014","journal-title":"Hum. Genomics"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1016\/S0165-0327(02)00426-3","article-title":"Epidemiology of women and depression","volume":"74","author":"Kessler","year":"2003","journal-title":"J. Affect. Disord."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1037\/1528-3542.6.1.139","article-title":"A population approach to the study of emotion: Diurnal rhythms of a working day examined with the day reconstruction method","volume":"6","author":"Stone","year":"2006","journal-title":"Emotion"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1309","DOI":"10.1016\/j.neuroscience.2005.05.003","article-title":"Effect of fluoxetine and cocaine on the expression of clock genes in the mouse hippocampus and striatum","volume":"134","author":"Uz","year":"2005","journal-title":"Neuroscience"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1002\/ajmg.b.30130","article-title":"Insomnia improvement during antidepressant treatment and CLOCK gene polymorphism","volume":"137B","author":"Serretti","year":"2005","journal-title":"Am. J. Med. Genet. B"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1002\/ajmg.b.20038","article-title":"Influence of CLOCK gene polymorphism on circadian mood fluctuation and illness recurrence in bipolar depression","volume":"123","author":"Benedetti","year":"2003","journal-title":"Am. J. Med. Genet. Part B"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"525","DOI":"10.1016\/S0896-6273(01)00302-6","article-title":"Differential functions of mPer1, mPer2, and mPer3 in the SCN circadian clock","volume":"30","author":"Bae","year":"2001","journal-title":"Neuron"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1016\/j.tem.2009.12.011","article-title":"Interactions of the circadian CLOCK system and the HPA axis","volume":"21","author":"Nader","year":"2010","journal-title":"Trends Endocrinol. Metab."},{"key":"ref_21","first-page":"pt4","article-title":"Circadian rhythms of glucocorticoid hormone actions in target tissues: Potential clinical implications","volume":"5","author":"Kino","year":"2012","journal-title":"Sci. Signal."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1016\/S0149-7634(01)00010-0","article-title":"Animal models of anxiety and depression: How are females different","volume":"25","author":"Palanza","year":"2001","journal-title":"Neurosci. Biobehav. Rev."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"422","DOI":"10.1016\/j.yhbeh.2007.11.014","article-title":"Gonadectomy reveals sex differences in circadian rhythms and suprachiasmatic nucleus androgen receptors in mice","volume":"53","author":"Iwahana","year":"2008","journal-title":"Horm. Behav."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1016\/j.physbeh.2009.11.002","article-title":"Circadian phase and sex effects on depressive\/anxiety-like behaviors and HPA axis responses to acute stress","volume":"99","author":"Verma","year":"2010","journal-title":"Physiol. Behav."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1367","DOI":"10.1016\/j.cub.2004.07.055","article-title":"Circadian clock mutation disrupts estrous cyclicity and maintenance of pregnancy","volume":"14","author":"Miller","year":"2004","journal-title":"Curr. Biol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1530\/REP-07-0434","article-title":"Low reproductive success in Per1 and Per2 mutant mouse females due to accelerated ageing","volume":"135","author":"Pilorz","year":"2008","journal-title":"Reproduction"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"5591","DOI":"10.1073\/pnas.0601310103","article-title":"The expression of the clock protein PER2 in the limbic forebrain is modulated by the estrous cycle","volume":"103","author":"Perrin","year":"2006","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1016\/j.ejphar.2005.08.012","article-title":"Stressors affect the response of male and female rats to clomipramine in a model of behavioral despair (forced swim test)","volume":"520","author":"Consoli","year":"2005","journal-title":"Eur. J. Pharmacol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"436","DOI":"10.1016\/j.neuropharm.2011.08.025","article-title":"Behavioral sexual dimorphism in models of anxiety and depression due to changes in HPA axis activity","volume":"62","author":"Kokras","year":"2012","journal-title":"Neuropharmacology"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"730","DOI":"10.1038\/266730a0","article-title":"Depression: A new animal model sensitive to antidepressant treatments","volume":"266","author":"Porsolt","year":"1977","journal-title":"Nature"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1339","DOI":"10.1016\/S0028-3908(02)00330-1","article-title":"Behavioral action of ethanol in Porsolt\u2019s forced swim test: Modulation by 3 alpha-hydroxy-5 alpha-pregnan-20-one","volume":"43","author":"Hirani","year":"2002","journal-title":"Neuropharmacology"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"403","DOI":"10.3109\/07420529209064552","article-title":"Chronolab: An interactive software package for chronobiologic time series analysis written for the Macintosh computer","volume":"9","author":"Mojon","year":"1992","journal-title":"Chronobiol. Int."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/16\/4\/6831\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T20:43:55Z","timestamp":1760215435000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/16\/4\/6831"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,3,25]]},"references-count":32,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2015,4]]}},"alternative-id":["ijms16046831"],"URL":"https:\/\/doi.org\/10.3390\/ijms16046831","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,3,25]]}}}