{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T04:23:36Z","timestamp":1760243016110,"version":"build-2065373602"},"reference-count":25,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2015,4,13]],"date-time":"2015-04-13T00:00:00Z","timestamp":1428883200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"DaLin Tzu Chi Buddhist Hospital","award":["SP98-2-3 and DTCRD 103-E-14"],"award-info":[{"award-number":["SP98-2-3 and DTCRD 103-E-14"]}]},{"DOI":"10.13039\/501100004725","name":"Ministry of Economic Affairs","doi-asserted-by":"publisher","award":["103-EC-17-A-09-S1-206"],"award-info":[{"award-number":["103-EC-17-A-09-S1-206"]}],"id":[{"id":"10.13039\/501100004725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>BH2, a monoclonal antibody prepared against the denatured human leukocytic antigen-B27 heavy chain (HLA-B27 HC), can immunoprecipitate the misfolded HLA-B27 HC complexed with Bip in the endoplasmic reticulum and recognize the homodimerized HLA-B27 HC that is often observed on the cell membrane of patients suffered from ankylosing spondylitis (AS). However, the recognition specificity of BH2 toward the other molecules of HLA-B type and toward the different types of HLA molecules remained uncharacterized. In this study, we carried out the HLA-typing by using the Luminex Technology to characterize the recognition specificity of BH2 and analyzed the binding domain of HLA-B27 HC by BH2. Our results indicated that BH2 preferably binds to molecules of HLA-B and -C rather than HLA-A and the binding site is located within the \u03b12 domain of HLA-B27 HC.<\/jats:p>","DOI":"10.3390\/ijms16048142","type":"journal-article","created":{"date-parts":[[2015,4,14]],"date-time":"2015-04-14T03:11:46Z","timestamp":1428981106000},"page":"8142-8150","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Characterization of the Recognition Specificity of BH2,  a Monoclonal Antibody Prepared against the HLA-B27  Heavy Chain"],"prefix":"10.3390","volume":"16","author":[{"given":"Hui-Chun","family":"Yu","sequence":"first","affiliation":[{"name":"Department of Life Science and Institute of Molecular Biology, National Chung Cheng University, Chia-Yi 621, Taiwan"},{"name":"Section of Allergy, Immunology and Rheumatology, Department of Medicine,  Buddhist DaLin Tzu-Chi Hospital, Chia-Yi 622, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kuang-Yung","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Life Science and Institute of Molecular Biology, National Chung Cheng University, Chia-Yi 621, Taiwan"},{"name":"Section of Allergy, Immunology and Rheumatology, Department of Medicine,  Buddhist DaLin Tzu-Chi Hospital, Chia-Yi 622, Taiwan"},{"name":"School of Medicine, Tzu-Chi University, Hualien 970, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ming-Chi","family":"Lu","sequence":"additional","affiliation":[{"name":"Section of Allergy, Immunology and Rheumatology, Department of Medicine,  Buddhist DaLin Tzu-Chi Hospital, Chia-Yi 622, Taiwan"},{"name":"School of Medicine, Tzu-Chi University, Hualien 970, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hsien-Lu","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Nutrition and Health Science, Fooyin University, Kaohsiung 831, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei-Ting","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Life Science and Institute of Molecular Biology, National Chung Cheng University, Chia-Yi 621, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wen-Chien","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering, National Chung Cheng University, Chia-Yi 621, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Su-Qin","family":"Liu","sequence":"additional","affiliation":[{"name":"Section of Allergy, Immunology and Rheumatology, Department of Medicine,  Buddhist DaLin Tzu-Chi Hospital, Chia-Yi 622, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hsien-Bin","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Life Science and Institute of Molecular Biology, National Chung Cheng University, Chia-Yi 621, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ning-Sheng","family":"Lai","sequence":"additional","affiliation":[{"name":"Section of Allergy, Immunology and Rheumatology, Department of Medicine,  Buddhist DaLin Tzu-Chi Hospital, Chia-Yi 622, Taiwan"},{"name":"School of Medicine, Tzu-Chi University, Hualien 970, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2015,4,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1016\/0092-8674(81)90318-4","article-title":"Major histocompatibility antigens: The human (HLA-A, -B, -C) and murine (H-2K, H-2D) class I molecules","volume":"24","author":"Ploegh","year":"1981","journal-title":"Cell"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1146\/annurev.iy.11.040193.001241","article-title":"Peptide naturally presented by MHC class I molecules","volume":"11","author":"Rammensee","year":"1993","journal-title":"Annu. 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