{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T09:49:51Z","timestamp":1771667391454,"version":"3.50.1"},"reference-count":48,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2015,6,19]],"date-time":"2015-06-19T00:00:00Z","timestamp":1434672000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>MicroRNAs (miRNAs) are small non-coding RNAs that have shown promise  as noninvasive biomarkers in cardiac disease. This study was undertaken to investigate  the miRNA expression profile in dogs with myxomatous mitral valve disease (MMVD).  277 miRNAs were quantified using RT-qPCR from six normal dogs (American College of Veterinary Internal Medicine Stage A), six dogs with MMVD mild to moderate cardiac enlargement (ACVIM Stage B1\/B2) and six dogs with MMVD and congestive heart failure (ACVIM Stage C\/D). Eleven miRNAs were differentially expressed (False Discovery  Rate &lt; 0.05). Dogs in Stage B1\/B2 or C\/D had four upregulated miRNAs, including three cfa-let-7\/cfa-miR-98 family members, while seven others were downregulated, compared to Stage A. Expression of six of the 11 miRNAs also were significantly different between dogs in Stage C\/D and those in Stage B1\/B2. The expression changes were greater as disease severity increased. These miRNAs may be candidates for novel biomarkers and may provide insights into genetic regulatory pathways in canine MMVD.<\/jats:p>","DOI":"10.3390\/ijms160614098","type":"journal-article","created":{"date-parts":[[2015,6,19]],"date-time":"2015-06-19T10:23:33Z","timestamp":1434709413000},"page":"14098-14108","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["Expression Profiling of Circulating MicroRNAs in Canine Myxomatous Mitral Valve Disease"],"prefix":"10.3390","volume":"16","author":[{"given":"Qinghong","family":"Li","sequence":"first","affiliation":[{"name":"Nestle Purina Research, Saint Louis, MO 63164, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lisa","family":"Freeman","sequence":"additional","affiliation":[{"name":"Cummings School of Veterinary Medicine, Tufts University, North Grafton, MA 01536, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John","family":"Rush","sequence":"additional","affiliation":[{"name":"Cummings School of Veterinary Medicine, Tufts University, North Grafton, MA 01536, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dorothy","family":"Laflamme","sequence":"additional","affiliation":[{"name":"Nestle Purina Research, Saint Louis, MO 63164, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2015,6,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1016\/S0092-8674(04)00045-5","article-title":"MicroRNAs: Genomics, biogenesis, mechanism, and function","volume":"116","author":"Bartel","year":"2004","journal-title":"Cell"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"827","DOI":"10.1152\/physrev.00006.2010","article-title":"MicroRNAs in development and disease","volume":"91","author":"Sayed","year":"2011","journal-title":"Physiol. 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