{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T16:53:02Z","timestamp":1787849582610,"version":"build-2784847793"},"reference-count":56,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2015,7,30]],"date-time":"2015-07-30T00:00:00Z","timestamp":1438214400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Repair of oxidative DNA damage, particularly Base Excision Repair (BER), impairment is often associated with Alzheimer\u2019s disease pathology. Here, we aimed at investigating the complete Nucleotide Excision Repair (NER), a DNA repair pathway involved in the removal of bulky DNA adducts, status in an Alzheimer-like cell line. The level of DNA damage was quantified using mass spectrometry, NER gene expression was assessed by qPCR, and the NER protein activity was analysed through a modified version of the COMET assay. Interestingly, we found that in the presence of the Amyloid \u03b2 peptide (A\u03b2), NER factors were upregulated at the mRNA level and that NER capacities were also specifically increased following oxidative stress. Surprisingly, NER capacities were not differentially improved following a typical NER-triggering of ultraviolet C (UVC) stress. Oxidative stress generates a differential and specific DNA damage response in the presence of A\u03b2. We hypothesized that the release of NER components such as DNA damage binding protein 2 (DDB2) and Xeroderma Pigmentosum complementation group C protein (XPC) following oxidative stress might putatively involve their apoptotic role rather than DNA repair function.<\/jats:p>","DOI":"10.3390\/ijms160817422","type":"journal-article","created":{"date-parts":[[2015,7,30]],"date-time":"2015-07-30T11:21:59Z","timestamp":1438255319000},"page":"17422-17444","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Combination of A\u03b2 Secretion and Oxidative Stress in an Alzheimer-Like Cell Line Leads to the Over-Expression of the Nucleotide Excision Repair Proteins DDB2 and XPC"],"prefix":"10.3390","volume":"16","author":[{"given":"Anne","family":"Forestier","sequence":"first","affiliation":[{"name":"Laboratoire L\u00e9sions des Acides Nucl\u00e9iques, Universit\u00e9 Joseph Fourier-Grenoble 1\/CEA\/Institut Nanoscience et Cryog\u00e9nie\/SCIB, UMR-E3, Grenoble, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thierry","family":"Douki","sequence":"additional","affiliation":[{"name":"Laboratoire L\u00e9sions des Acides Nucl\u00e9iques, Universit\u00e9 Joseph Fourier-Grenoble 1\/CEA\/Institut Nanoscience et Cryog\u00e9nie\/SCIB, UMR-E3, Grenoble, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Viviana","family":"De Rosa","sequence":"additional","affiliation":[{"name":"Laboratoire L\u00e9sions des Acides Nucl\u00e9iques, Universit\u00e9 Joseph Fourier-Grenoble 1\/CEA\/Institut Nanoscience et Cryog\u00e9nie\/SCIB, UMR-E3, Grenoble, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"B\u00e9al","sequence":"additional","affiliation":[{"name":"Laboratoire L\u00e9sions des Acides Nucl\u00e9iques, Universit\u00e9 Joseph Fourier-Grenoble 1\/CEA\/Institut Nanoscience et Cryog\u00e9nie\/SCIB, UMR-E3, Grenoble, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Walid","family":"Rachidi","sequence":"additional","affiliation":[{"name":"Laboratoire L\u00e9sions des Acides Nucl\u00e9iques, Universit\u00e9 Joseph Fourier-Grenoble 1\/CEA\/Institut Nanoscience et Cryog\u00e9nie\/SCIB, UMR-E3, Grenoble, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2015,7,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"631","DOI":"10.1038\/nature02621","article-title":"Pathways towards and away from Alzheimer\u02bcs disease","volume":"430","author":"Mattson","year":"2004","journal-title":"Nature"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1371","DOI":"10.1007\/s10495-010-0501-0","article-title":"DNA damage, neuronal and glial cell death and neurodegeneration","volume":"15","author":"Barzilai","year":"2010","journal-title":"Apoptosis"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.molbrainres.2004.05.023","article-title":"Deficiency of the Mre11 DNA repair complex in Alzheimer\u02bcs disease brains","volume":"128","author":"Jacobsen","year":"2004","journal-title":"Brain Res. 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