{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T06:20:52Z","timestamp":1777616452444,"version":"3.51.4"},"reference-count":19,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2015,10,13]],"date-time":"2015-10-13T00:00:00Z","timestamp":1444694400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the National Basic Research and Development Program of China","award":["2012CB518805"],"award-info":[{"award-number":["2012CB518805"]}]},{"name":"the Research and Development Program of Jiangsu","award":["BE2015343"],"award-info":[{"award-number":["BE2015343"]}]},{"name":"the NSF of Yangzhou","award":["YZ2014027"],"award-info":[{"award-number":["YZ2014027"]}]},{"name":"the Priority Academic Development Program of Jiangsu Higher Education Institutions"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Macrophages (M\u03a6) and dendritic cells (DCs) are both pivotal antigen presenting cells capable of inducing specific cellular responses to inhaled mycobacteria, and thus,  they may be important in the initiation of early immune responses to mycobacterial infection.  In this study, we evaluated and compared the roles of murine splenic DCs and M\u03a6s in  immunity against Mycobacterium bovis Bacillus Calmette-Gu\u00e9rin (M.bovis BCG). The number of internalized rBCG-GFP observed was obviously greater in murine splenic M\u03a6s compared with DCs, and the intracellular reactive oxygen species (ROS), inducible nitric oxide synthase (iNOS) and nitric oxide (NO) levels in M\u03a6s were all higher than in DCs. DCs have  a stronger capacity for presenting Ag85A peptide to specific T hybridoma and when the  murine splenic M\u03a6s were infected with BCG and rBCG::Ag85A, low level of antigen presenting activity was detected. These data suggest that murine splenic M\u03a6s participate in mycobacteria uptake, killing and inducing inflammatory response, whereas the murine splenic DCs are primarily involved in specific antigen presentation and T cell activation.<\/jats:p>","DOI":"10.3390\/ijms161024127","type":"journal-article","created":{"date-parts":[[2015,10,14]],"date-time":"2015-10-14T02:36:30Z","timestamp":1444790190000},"page":"24127-24138","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Differential Effects of Mycobacterium bovis BCG on Macrophages and Dendritic Cells from Murine Spleen"],"prefix":"10.3390","volume":"16","author":[{"given":"Zhengzhong","family":"Xu","sequence":"first","affiliation":[{"name":"Jiangsu Key Laboratory of Zoonosis\/Jiangsu Co-Innovation Center for Prevention and  Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University,  Yangzhou 225009, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chuang","family":"Meng","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Zoonosis\/Jiangsu Co-Innovation Center for Prevention and  Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University,  Yangzhou 225009, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bin","family":"Qiang","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Zoonosis\/Jiangsu Co-Innovation Center for Prevention and  Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University,  Yangzhou 225009, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongyan","family":"Gu","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Zoonosis\/Jiangsu Co-Innovation Center for Prevention and  Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University,  Yangzhou 225009, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lin","family":"Sun","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Zoonosis\/Jiangsu Co-Innovation Center for Prevention and  Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University,  Yangzhou 225009, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuelan","family":"Yin","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Zoonosis\/Jiangsu Co-Innovation Center for Prevention and  Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University,  Yangzhou 225009, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhiming","family":"Pan","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Zoonosis\/Jiangsu Co-Innovation Center for Prevention and  Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University,  Yangzhou 225009, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiang","family":"Chen","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Zoonosis\/Jiangsu Co-Innovation Center for Prevention and  Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University,  Yangzhou 225009, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xinan","family":"Jiao","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Zoonosis\/Jiangsu Co-Innovation Center for Prevention and  Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University,  Yangzhou 225009, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2015,10,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"494","DOI":"10.4049\/jimmunol.173.1.494","article-title":"Dendritic cell trafficking and antigen presentation in the human immune response to Mycobacterium tuberculosis","volume":"173","author":"Marino","year":"2004","journal-title":"J. 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