{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T06:59:01Z","timestamp":1785826741008,"version":"3.56.0"},"reference-count":58,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2015,10,22]],"date-time":"2015-10-22T00:00:00Z","timestamp":1445472000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"Natural Science Foundation of China","doi-asserted-by":"publisher","award":["U1304319"],"award-info":[{"award-number":["U1304319"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Open Topic of National Key Laboratory of Cotton Biology, CICAAS","award":["CB2013A03"],"award-info":[{"award-number":["CB2013A03"]}]},{"name":"National Transgenic Major Projects","award":["2014ZX08005005-006"],"award-info":[{"award-number":["2014ZX08005005-006"]}]},{"name":"Henan Provincial Budget Project","award":["2012-2014"],"award-info":[{"award-number":["2012-2014"]}]},{"name":"Innovative Talents of Science and Technology Plan of Henan Province","award":["2015-2017"],"award-info":[{"award-number":["2015-2017"]}]},{"name":"International Training of Henan Provincial High-level Talents Training","award":["YWZ[2014]2"],"award-info":[{"award-number":["YWZ[2014]2"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Verticillium wilt is threatening cotton productivity globally. This disease  is caused by soil-borne Verticillium dahliae which directly infects cotton roots, and exclusively colonizes and occludes xylem vessels, finally resulting in necrosis, defoliation, and most severely, plant death. For the first time, iTRAQ (isobaric tags for relative and absolute quantification) was applied to screen the differentially expressed proteins of Gossypium thurberi inoculated with V. dahliae. A total of 6533 proteins were identified  from the roots of G. thurberi after inoculation with V. dahliae, and 396 showed up- and  279 down-regulated in comparison to a mock-inoculated roots. Of these identified  proteins, the main functional groups were those involved in cell wall organization  and reinforcement, disease-resistant chemicals of secondary metabolism, phytohormone signaling, pathogenesis-related proteins, and disease-resistant proteins. Physiological and biochemical analysis showed that peroxidase activity, which promotes the biosynthesis and accumulation of lignin, was induced early in the hypocotyl after inoculation with V. dahliae. Similarly, salicylic acid also accumulated significantly in hypocotyl of the seedlings after inoculation. These findings provide an important knowledge of the molecular events and regulatory networks occurring during G. thurberi-V. dahliae interaction, which may provide a foundation for breeding disease-resistance in cotton.<\/jats:p>","DOI":"10.3390\/ijms161025121","type":"journal-article","created":{"date-parts":[[2015,10,26]],"date-time":"2015-10-26T04:12:04Z","timestamp":1445832724000},"page":"25121-25140","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Comparative Proteomic Analysis of Gossypium thurberi in Response to Verticillium dahliae Inoculation"],"prefix":"10.3390","volume":"16","author":[{"given":"Weiping","family":"Fang","sequence":"first","affiliation":[{"name":"Economic Crop Research Institute, Henan Academy of Agricultural Sciences,  Zhengzhou 450002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deyi","family":"Xie","sequence":"additional","affiliation":[{"name":"Economic Crop Research Institute, Henan Academy of Agricultural Sciences,  Zhengzhou 450002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heqin","family":"Zhu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wu","family":"Li","sequence":"additional","affiliation":[{"name":"Economic Crop Research Institute, Henan Academy of Agricultural Sciences,  Zhengzhou 450002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenzhen","family":"Xu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, 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