{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T10:33:47Z","timestamp":1788345227437,"version":"build-2803163510"},"reference-count":51,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2015,11,16]],"date-time":"2015-11-16T00:00:00Z","timestamp":1447632000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>In this review, we disclose a selection of serum\/plasma biomarkers of (anti)oxidant status related to nutrition, which can be used for measurements in large-scale epidemiological studies. From personal experience, we have come to the following proposal of a set of biomarkers for nutritional intake, (anti)oxidant status, and redox status. We have selected the individual antioxidant vitamins E and A, and the carotenoids which can be measured in large series by HPLC. In addition, vitamin C was selected, which can be measured by an auto-analyzer or HPLC. As a biomarker for oxidative stress, the ROM assay (reactive oxygen metabolites) was selected; for the redox status, the total thiol assay; and for the total antioxidant status the BAP assay (biological antioxidant potential). All of these biomarkers can be measured in large quantities by an auto-analyzer. Critical points in biomarker validation with respect to blood sampling, storage conditions, and measurements are discussed. With the selected biomarkers, a good set is presented for use in the risk assessment between nutrition and (chronic) diseases in large-scale epidemiological studies. Examples of the successful application of these biomarkers in large international studies are presented.<\/jats:p>","DOI":"10.3390\/ijms161126032","type":"journal-article","created":{"date-parts":[[2015,11,16]],"date-time":"2015-11-16T10:17:10Z","timestamp":1447669030000},"page":"27378-27390","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":48,"title":["Serum Biomarkers of (Anti)Oxidant Status for Epidemiological Studies"],"prefix":"10.3390","volume":"16","author":[{"given":"Eug\u00e8ne","family":"Jansen","sequence":"first","affiliation":[{"name":"Centre for Health Protection, National Institute for Public Health and the Environment, PO Box 1, 3720 BA Bilthoven, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4300-4891","authenticated-orcid":false,"given":"Tatjana","family":"Ruskovska","sequence":"additional","affiliation":[{"name":"Faculty of Medical Sciences, Goce Delcev University, 2000 Stip, Macedonia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2015,11,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1189","DOI":"10.3945\/ajcn.2010.29673","article-title":"Accruing evidence on benefits of adherence to the Mediterranean diet on health: An updated systematic review and meta-analysis","volume":"92","author":"Sofi","year":"2010","journal-title":"Am. 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