{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,10]],"date-time":"2026-04-10T23:08:15Z","timestamp":1775862495225,"version":"3.50.1"},"reference-count":22,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2016,1,7]],"date-time":"2016-01-07T00:00:00Z","timestamp":1452124800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The goal to successful wound healing is essentially to immobilize and recruit appropriate numbers of host stem or progenitor cells to the wound area. In this study, we developed a chitosan nanofiber-immobilized neuropeptide substance-P (SP), which mediates stem cell mobilization and migration, onto the surfaces of nanofibers using a peptide-coupling agent, and evaluated its biological effects on stem cells. The amount of immobilized SP on chitosan nanofibers was modulated over the range of 5.89 \u00b1 3.27 to 75.29 \u00b1 24.31 ng when reacted with 10 to 500 ng SP. In vitro migration assays showed that SP-incorporated nanofibers induced more rapid migration of human mesenchymal stem cells on nanofibers compared to pristine samples. Finally, the conjugated SP evoked a minimal foreign body reaction and recruited a larger number of CD29- and CD44-positive stem cells into nanofibers in a mouse subcutaneous pocket model.<\/jats:p>","DOI":"10.3390\/ijms17010068","type":"journal-article","created":{"date-parts":[[2016,1,7]],"date-time":"2016-01-07T11:11:51Z","timestamp":1452165111000},"page":"68","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Neuropeptide Substance-P-Conjugated Chitosan Nanofibers as an Active Modulator of Stem Cell Recruiting"],"prefix":"10.3390","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8050-7714","authenticated-orcid":false,"given":"Min","family":"Kim","sequence":"first","affiliation":[{"name":"Laboratory of Tissue Engineering, Korea Institute of Radiological and Medical Science, Seoul 139240, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sang","family":"Park","sequence":"additional","affiliation":[{"name":"Laboratory of Tissue Engineering, Korea Institute of Radiological and Medical Science, Seoul 139240, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wheemoon","family":"Cho","sequence":"additional","affiliation":[{"name":"Laboratory of Tissue Engineering, Korea Institute of Radiological and Medical Science, Seoul 139240, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bon","family":"Gu","sequence":"additional","affiliation":[{"name":"Laboratory of Tissue Engineering, Korea Institute of Radiological and Medical Science, Seoul 139240, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chun-Ho","family":"Kim","sequence":"additional","affiliation":[{"name":"Laboratory of Tissue Engineering, Korea Institute of Radiological and Medical Science, Seoul 139240, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2016,1,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1126\/science.276.5309.75","article-title":"Wound healing\u2014Aiming for perfect skin regeneration","volume":"276","author":"Martin","year":"1997","journal-title":"Science"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"12S","DOI":"10.1097\/01.prs.0000225430.42531.c2","article-title":"The basic science of wound healing","volume":"117","author":"Broughton","year":"2006","journal-title":"Plast. 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