{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T19:18:22Z","timestamp":1772824702454,"version":"3.50.1"},"reference-count":68,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2016,6,28]],"date-time":"2016-06-28T00:00:00Z","timestamp":1467072000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Defective cellular trafficking of aquaporin-5 (AQP5) to the apical plasma membrane (APM) in salivary glands is associated with the loss of salivary fluid secretion. To examine mechanisms of \u03b11-adrenoceptor (AR)-induced trafficking of AQP5, immunoconfocal microscopy and Western blot analysis were used to analyze AQP5 localization in parotid tissues stimulated with phenylephrine under different osmolality. Phenylephrine-induced trafficking of AQP5 to the APM and lateral plasma membrane (LPM) was mediated via the \u03b11A-AR subtype, but not the \u03b11B- and \u03b11D-AR subtypes. Phenylephrine-induced trafficking of AQP5 was inhibited by ODQ and KT5823, inhibitors of nitric oxide (NO)-stimulated guanylcyclase (GC) and protein kinase (PK) G, respectively, indicating the involvement of the NO\/ soluble (c) GC\/PKG signaling pathway. Under isotonic conditions, phenylephrine-induced trafficking was inhibited by La3+, implying the participation of store-operated Ca2+ channel. Under hypotonic conditions, phenylephrine-induced trafficking of AQP5 to the APM was higher than that under isotonic conditions. Under non-stimulated conditions, hypotonicity-induced trafficking of AQP5 to the APM was inhibited by ruthenium red and La3+, suggesting the involvement of extracellular Ca2+ entry. Thus, \u03b11A-AR activation induced the trafficking of AQP5 to the APM and LPM via the Ca2+\/ cyclic guanosine monophosphate (cGMP)\/PKG signaling pathway, which is associated with store-operated Ca2+ entry.<\/jats:p>","DOI":"10.3390\/ijms17071022","type":"journal-article","created":{"date-parts":[[2016,6,29]],"date-time":"2016-06-29T06:20:33Z","timestamp":1467181233000},"page":"1022","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Mechanisms Underlying Activation of \u03b11-Adrenergic Receptor-Induced Trafficking of AQP5 in Rat Parotid Acinar Cells under Isotonic or Hypotonic Conditions"],"prefix":"10.3390","volume":"17","author":[{"given":"Aneta","family":"Bragiel","sequence":"first","affiliation":[{"name":"Department of Medical Pharmacology, Institute of Biomedical Sciences, Tokushima University Graduate School, 3-18-15, Kuramoto-cho, Tokushima 770-8504, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Di","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Medical Pharmacology, Institute of Biomedical Sciences, Tokushima University Graduate School, 3-18-15, Kuramoto-cho, Tokushima 770-8504, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomasz","family":"Pieczonka","sequence":"additional","affiliation":[{"name":"Department of Medical Pharmacology, Institute of Biomedical Sciences, Tokushima University Graduate School, 3-18-15, Kuramoto-cho, Tokushima 770-8504, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masayuki","family":"Shono","sequence":"additional","affiliation":[{"name":"Support Center for Advanced Medical Sciences, Institute of Biomedical Sciences, Tokushima University Graduate School, 3-18-15, Kuramoto-cho, Tokushima 770-8504, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1689-2827","authenticated-orcid":false,"given":"Yasuko","family":"Ishikawa","sequence":"additional","affiliation":[{"name":"Department of Medical Pharmacology, Institute of Biomedical Sciences, Tokushima University Graduate School, 3-18-15, Kuramoto-cho, Tokushima 770-8504, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2016,6,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/j.autneu.2006.10.006","article-title":"Regulation of salivary gland function by autonomic nerves","volume":"133","author":"Proctor","year":"2007","journal-title":"Auton. Neurosci."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1016\/j.ceca.2014.02.009","article-title":"Ca2+ signaling and regulation of fluid secretion in salivary gland acinar cells","volume":"55","author":"Ambudkar","year":"2014","journal-title":"Cell Calcium"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1111\/j.1365-2796.2006.01760.x","article-title":"Regulation and dysregulation of aquaporins in water balance disorders","volume":"261","author":"Nielsen","year":"2007","journal-title":"J. Intern. Med."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"R566","DOI":"10.1152\/ajpregu.90464.2008","article-title":"The evolutionary aspects of aquaporin family","volume":"300","author":"Ishibashi","year":"2011","journal-title":"Am. J. Physiol. Regul. Integr. Comp. Physiol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1908","DOI":"10.1074\/jbc.270.4.1908","article-title":"Molecular cloning and characterization of an aquaporin cDNA from salivary, lacrimal, and respiratory tissues","volume":"270","author":"Raina","year":"1995","journal-title":"J. Biol. Chem."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"23413","DOI":"10.1074\/jbc.M008760200","article-title":"Salivary acinar cells from aquaporin 5-deficient mice have decreased membrane water permeability and altered cell volume regulation","volume":"276","author":"Krane","year":"2001","journal-title":"J. Biol. Chem."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"715","DOI":"10.1177\/0022034515570943","article-title":"Functional differences in the acinar cells of the murine major salivary glands","volume":"94","author":"Kondo","year":"2015","journal-title":"J. Dent. Res."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"835","DOI":"10.1006\/bbrc.1998.8395","article-title":"Acetylcholine acts on M3 muscarinic receptors and induces the translocation of aquaporin5 water channel via cytosolic Ca2+ elevation in rat parotid glands","volume":"245","author":"Ishikawa","year":"1998","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1006\/bbrc.1999.1630","article-title":"\u03b11-adrenoceptor-induced trafficking of aquaporin-5 to the apical plasma membrane of rat parotid cells","volume":"265","author":"Ishikawa","year":"1999","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1053","DOI":"10.1016\/j.bbamem.2006.03.026","article-title":"Aquaporin-5 water channel in lipid rafts of rat parotid glands","volume":"1758","author":"Ishikawa","year":"2006","journal-title":"Biochim. Biophys. Acta"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"784","DOI":"10.1016\/j.bbagen.2015.01.009","article-title":"Activation of muscarinic receptors in rat parotid acinar cells induces AQP5 trafficking to nuclei and apical plasma membrane","volume":"1850","author":"Cho","year":"2015","journal-title":"Biochim. Biophys. Acta"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"C1303","DOI":"10.1152\/ajpcell.00211.2005","article-title":"Identification of AQP5 in lipid rafts and its translocation to apical membranes by activation of M3 mAChRs in interlobular ducts of rat parotid gland","volume":"289","author":"Ishikawa","year":"2005","journal-title":"Am. J. Physiol. Cell Physiol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1016\/j.bbagen.2008.08.009","article-title":"Identification of aquaporin-5 and lipid rafts in human resting saliva and their release into cevimeline-stimulated saliva","volume":"1790","author":"Pan","year":"2009","journal-title":"Biochim. Biophys. Acta"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1248\/bpb.34.13","article-title":"Proteomic analysis of two types of exosomes in human whole saliva","volume":"34","author":"Ogawa","year":"2011","journal-title":"Biol. Pharm. Bull."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1016\/S0031-6997(25)06845-0","article-title":"International Union of Pharmacology. X. Recommendation for nomenclature of \u03b1 1-adrenoceptors: Consensus update","volume":"47","author":"Hieble","year":"1995","journal-title":"Pharmacol. Rev."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1062","DOI":"10.1016\/S0022-3565(25)10440-0","article-title":"Atypical \u03b1-1 adrenergic receptors on the rat parotid gland acinar cell","volume":"263","author":"Porter","year":"1992","journal-title":"J. Pharmacol. Exp. Ther."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1177","DOI":"10.1006\/bbrc.1994.1575","article-title":"Distribution of \u03b11C-adrenergic receptor mRNA in adult rat tissues by RNase protection assay and comparison with \u03b11B and \u03b11D","volume":"200","author":"Rokosh","year":"1994","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1016\/j.ejphar.2015.07.037","article-title":"Effect of silodosin, a selective \u03b11A-adrenoceptor antagonist, on voiding behavior and bladder blood flow in a rat model of bladder outlet obstruction","volume":"764","author":"Goi","year":"2015","journal-title":"Eur. J. Pharmacol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1019","DOI":"10.1111\/j.1464-410X.2006.06448.x","article-title":"Silodosin, a new \u03b11A-adrenoceptor-selective antagonist for treating benign prostatic hyperplasia: Results of a phase III randomized, placebo-controlled, double-blind study in Japanese men","volume":"98","author":"Kawabe","year":"2006","journal-title":"BJU Int."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/S0022-3565(24)35074-8","article-title":"KMD-3213, a uroselective and long-acting \u03b11A-adrenoceptor antagonist, tested in a novel rat model","volume":"291","author":"Akiyama","year":"1999","journal-title":"J. Pharmacol. Exp. Ther."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Ozsoy, M., Liatsikos, E., Scheffbuch, N., and Kallidonis, P. (2016). Comparison of silodosin to tamsulosin for medical expulsive treatment of ureteral stones: A systematic review and meta-analysis. Urolithiasis, in press.","DOI":"10.1007\/s00240-016-0872-y"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"256","DOI":"10.1016\/j.ejphar.2007.11.003","article-title":"In vivo study on the effects of \u03b11-adrenoceptor antagonists on intraurethral pressure in the prostatic urethra and intraluminal pressure in the vas deferens in male dogs","volume":"580","author":"Noguchi","year":"2008","journal-title":"Eur. J. Pharmacol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"419","DOI":"10.1016\/j.ejmech.2014.06.057","article-title":"High affinity ligands and potent antagonists for the \u03b11D-adrenergic receptor. Novel 3,8-disubstituted [1]benzothieno[3,2-d]pyrimidine derivatives","volume":"83","author":"Romeo","year":"2014","journal-title":"Eur. J. Med. Chem."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1016\/j.ejphar.2012.01.019","article-title":"Effects of \u03b11-adrenoceptor antagonists on phenylephrine-induced salivary secretion and intraurethral pressure elevation in anesthetized rats","volume":"679","author":"Ohtake","year":"2012","journal-title":"Eur. J. Pharmacol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"2087","DOI":"10.1074\/jbc.275.3.2087","article-title":"Constitutively active mutants of the \u03b11A- and the \u03b11B-adrenergic receptor subtypes reveal coupling to different signaling pathways and physiological responses in rat cardiac myocytes","volume":"275","author":"McWhinney","year":"2000","journal-title":"J. Biol. Chem."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1016\/j.ejphar.2007.09.029","article-title":"Characterization of the \u03b11-adrenoceptor subtype activating extracellular signal-regulated kinase in submandibular gland acinar cells","volume":"578","author":"Bruchas","year":"2008","journal-title":"Eur. J. Pharmacol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3109\/09687688.2012.743194","article-title":"An emerging consensus on aquaporin translocation as a regulatory mechanism","volume":"30","author":"Conner","year":"2013","journal-title":"Mol. Membr. Biol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"15485","DOI":"10.1074\/jbc.M600549200","article-title":"A role for AQP5 in activation of TRPV4 by hypotonicity: Concerted involvement of AQP5 and TRPV4 in regulation of cell volume recovery","volume":"281","author":"Liu","year":"2006","journal-title":"J. Biol. Chem."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"4747","DOI":"10.1073\/pnas.0511211103","article-title":"Transient receptor potential vanilloid 4 regulates aquaporin-5 abundance under hypotonic conditions","volume":"103","author":"Sidhaye","year":"2006","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"2571","DOI":"10.1007\/s00424-015-1720-6","article-title":"Regulation of the perilymphatic-endolymphatic water shunt in the cochlea by membrane translocation of aquaporin-5","volume":"467","author":"Eckhard","year":"2015","journal-title":"Pflug. Arch."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Kitchen, P., Oberg, F., Sjohamn, J., Hedfalk, K., Bill, R.M., Conner, A.C., Conner, M.T., and Tornroth-Horsefield, S. (2015). Plasma membrane abundance of human aquaporin 5 is dynamically regulated by multiple pathways. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0143027"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1111\/j.1600-0722.2010.00738.x","article-title":"Intracellular Ca2+ responses and cell volume regulation upon cholinergic and purinergic stimulation in an immortalized salivary cell line","volume":"118","author":"Aure","year":"2010","journal-title":"Eur. J. Oral. Sci."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1126\/science.1174621","article-title":"Lipid rafts as a membrane-organizing principle","volume":"327","author":"Lingwood","year":"2010","journal-title":"Science"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"318","DOI":"10.1016\/j.ab.2010.02.036","article-title":"Reversible Ponceau staining as a loading control alternative to actin in Western blots","volume":"401","author":"Ocon","year":"2010","journal-title":"Anal. Biochem."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"549","DOI":"10.1586\/14789450.2014.939635","article-title":"The necessity of and strategies for improving confidence in the accuracy of western blots","volume":"11","author":"Ghosh","year":"2014","journal-title":"Expert Rev. Proteom."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"26552","DOI":"10.1074\/jbc.M703158200","article-title":"Ankyrin-G is a molecular partner of E-cadherin in epithelial cells and early embryos","volume":"282","author":"Kizhatil","year":"2007","journal-title":"J. Biol. Chem."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1423","DOI":"10.1124\/mol.61.6.1423","article-title":"The muscarinic acetylcholine receptor-stimulated increase in aquaporin-5 levels in the apical plasma membrane in rat parotid acinar cells is coupled with activation of nitric oxide\/cGMP signal transduction","volume":"61","author":"Ishikawa","year":"2002","journal-title":"Mol. Pharmacol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"757","DOI":"10.1152\/physrev.00057.2003","article-title":"Store-operated calcium channels","volume":"85","author":"Parekh","year":"2005","journal-title":"Physiol. Rev."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1016\/0922-4106(91)90028-G","article-title":"Protein kinase C-dependent diacylglycerol formation is mediated via Ca2+\/calmodulin in parotid cells","volume":"207","author":"Komabayashi","year":"1991","journal-title":"Eur. J. Pharmacol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/S0143-4160(98)90074-4","article-title":"Calcium store depletion activates two distinct calcium entry pathways in secretory cells of the blowfly salivary gland","volume":"23","author":"Zimmermann","year":"1998","journal-title":"Cell Calcium"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"497","DOI":"10.1016\/S0896-6273(03)00462-8","article-title":"Hypotonicity induces TRPV4-mediated nociception in rat","volume":"39","author":"Yeh","year":"2003","journal-title":"Neuron"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"G1365","DOI":"10.1152\/ajpgi.00366.2012","article-title":"TRPV4 activation in mouse submandibular gland modulates Ca2+ influx and salivation","volume":"303","author":"Zhang","year":"2012","journal-title":"Am. J. Physiol. Gastrointest. Liver Physiol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1007\/BF00374318","article-title":"Quantitative estimation of the area of luminal and basolateral membranes of rat parotid acinar cells: Some physiological applications","volume":"429","author":"Poulsen","year":"1994","journal-title":"Pflug. Arch."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"3621","DOI":"10.1073\/pnas.0608384104","article-title":"Interaction between transcellular and paracellular water transport pathways through Aquaporin 5 and the tight junction complex","volume":"104","author":"Kawedia","year":"2007","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1007\/s00018-009-0174-4","article-title":"Subtypes of functional \u03b11-adrenoceptor","volume":"67","author":"Docherty","year":"2010","journal-title":"Cell. Mol. Life Sci."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"2873","DOI":"10.1523\/JNEUROSCI.22-07-02873.2002","article-title":"\u03b11B-adrenergic receptors control locomotor and rewarding effects of psychostimulants and opiates","volume":"22","author":"Drouin","year":"2002","journal-title":"J. Neurosci."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"9675","DOI":"10.1007\/s11357-014-9675-7","article-title":"Long-term \u03b11B-adrenergic receptor activation shortens lifespan, while \u03b11A-adrenergic receptor stimulation prolongs lifespan in association with decreased cancer incidence","volume":"36","author":"Collette","year":"2014","journal-title":"Age"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"765","DOI":"10.1172\/JCI200214001","article-title":"The \u03b11D-adrenergic receptor directly regulates arterial blood pressure via vasoconstriction","volume":"109","author":"Tanoue","year":"2002","journal-title":"J. Clin. Investig."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1281","DOI":"10.1111\/j.1745-7254.2005.00224.x","article-title":"Recent progress in \u03b11-adrenergic receptor research","volume":"26","author":"Chen","year":"2005","journal-title":"Acta Pharmacol. Sin."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1016\/j.eururo.2005.05.009","article-title":"Treatment of overactive bladder in the older patient: Pooled analysis of three phase III studies of darifenacin, an M3 selective receptor antagonist","volume":"48","author":"Foote","year":"2005","journal-title":"Eur. Urol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1016\/j.autneu.2009.12.005","article-title":"Differences in the Ca2+ response resulting from neurotransmitter stimulations of rat parotid acini and ducts","volume":"154","author":"Inagaki","year":"2010","journal-title":"Auton. Neurosci."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1054\/ceca.2001.0218","article-title":"Ca2+-regulated nitric oxide generation in rabbit parotid acinar cells","volume":"30","author":"Sugiya","year":"2001","journal-title":"Cell Calcium"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/S0014-2999(02)01375-4","article-title":"Activation of nitric oxide synthase through muscarinic receptors in rat parotid gland","volume":"439","author":"Rosignoli","year":"2002","journal-title":"Eur. J. Pharmacol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1016\/S0031-6997(24)01457-1","article-title":"Guanylyl cyclases and signaling by cyclic GMP","volume":"52","author":"Lucas","year":"2000","journal-title":"Pharmacol. Rev."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"16873","DOI":"10.1074\/jbc.M115.646034","article-title":"Identification and molecular mechanisms of the rapid tonicity-induced relocalization of the Aquaporin 4 channel","volume":"290","author":"Kitchen","year":"2015","journal-title":"J. Biol. Chem."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"3968","DOI":"10.1021\/pr800245x","article-title":"Author information. Pronounced alterations of cellular metabolism and structure due to hyper- or hypo-osmosis","volume":"7","author":"Mao","year":"2008","journal-title":"J. Proteome Res."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"579","DOI":"10.1016\/j.bbrc.2007.01.026","article-title":"Nitric oxide decreases cell surface expression of aquaporin-5 and membrane water permeability in lung epithelial cells","volume":"354","author":"Nagai","year":"2007","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"13337","DOI":"10.1074\/jbc.M110.112094","article-title":"Isoproterenol and cAMP block ERK phosphorylation and enhance [Ca2+]i increases and oxygen consumption by muscarinic receptor stimulation in rat parotid and submandibular acinar cells","volume":"285","author":"Soltoff","year":"2010","journal-title":"J. Biol. Chem."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1016\/S0014-2999(98)00651-7","article-title":"Activation of \u03b2-adrenoceptors does not cause any change in cytosolic Ca2+ distribution in rat parotid acinar cells","volume":"360","author":"Tojyo","year":"1998","journal-title":"Eur. J. Pharmacol."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"519","DOI":"10.1016\/0143-4160(89)90013-4","article-title":"Does \u03b2-adrenoceptor activation stimulate Ca2+ mobilization and inositol trisphosphate formation in parotid acinar cells?","volume":"10","author":"Hughes","year":"1989","journal-title":"Cell Calcium"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"625","DOI":"10.1016\/j.ceca.2009.02.009","article-title":"Lipid rafts\/caveolae as microdomains of calcium signaling","volume":"45","author":"Pani","year":"2009","journal-title":"Cell Calcium"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"667","DOI":"10.1242\/jcs.118943","article-title":"Impairment of TRPC1-STIM1 channel assembly and AQP5 translocation compromise agonist-stimulated fluid secretion in mice lacking caveolin1","volume":"126","author":"Pani","year":"2013","journal-title":"J. Cell Sci."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1679\/aohc.66.45","article-title":"Co-localization of rab4 with endocytosis-related proteins in the rat parotid glands","volume":"66","author":"Nashida","year":"2003","journal-title":"Arch. Histol. Cytol."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"791","DOI":"10.1097\/00004647-199707000-00009","article-title":"NG-nitro-L-arginine methyl ester modifies the input function measured by dynamic susceptibility contrast magnetic resonance imaging","volume":"17","author":"Mottet","year":"1997","journal-title":"J. Cereb. Blood Flow Metab."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1007\/s12576-011-0158-7","article-title":"Frequency components of systolic blood pressure variability reflect vasomotor and cardiac sympathetic functions in conscious rats","volume":"61","author":"Yoshimoto","year":"2011","journal-title":"J. Physiol. Sci."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1016\/0167-4889(89)90225-5","article-title":"The activation of rabbit intestinal adenylate cyclase by cholera toxin","volume":"1014","author":"Longbottom","year":"1989","journal-title":"Biochim. Biophys. Acta"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1016\/S0076-6879(81)77032-0","article-title":"\u03b3-Glutamyl transpeptidase","volume":"77","author":"Meister","year":"1981","journal-title":"Methods Enzymol."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1016\/j.brainres.2005.08.005","article-title":"High and low rearing subgroups of rats selected in the open field differ in the activity of K+-stimulated p-nitrophenylphosphatase in the hippocampus","volume":"1058","author":"Alves","year":"2005","journal-title":"Brain Res."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/17\/7\/1022\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T19:25:01Z","timestamp":1760210701000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/17\/7\/1022"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,6,28]]},"references-count":68,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2016,7]]}},"alternative-id":["ijms17071022"],"URL":"https:\/\/doi.org\/10.3390\/ijms17071022","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,6,28]]}}}