{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T14:16:31Z","timestamp":1761920191142,"version":"build-2065373602"},"reference-count":63,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2016,9,27]],"date-time":"2016-09-27T00:00:00Z","timestamp":1474934400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the Aid Program for Innovative Research Team in Shanxi Agricultural University","award":["CXTD201201"],"award-info":[{"award-number":["CXTD201201"]}]},{"name":"the High Technology Research and Development Program of China (863 Program)","award":["2013AA102506"],"award-info":[{"award-number":["2013AA102506"]}]},{"DOI":"10.13039\/501100001809","name":"the National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31302049"],"award-info":[{"award-number":["31302049"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"the Specialized Research Fund for the Doctoral Program of Higher Education","award":["20131403120002"],"award-info":[{"award-number":["20131403120002"]}]},{"name":"the Special Foundation for Agro-scientific Research in the Public Interest","award":["201303119"],"award-info":[{"award-number":["201303119"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>To investigate whether ocular albinism type 1 (OA1) is differentially expressed in the skin of mice with different coat colors and to determine its correlation with coat color establishment in mouse, the expression patterns and tissue distribution characterization of OA1 in the skin of mice with different coat colors were qualitatively and quantitatively analyzed by real-time quantitative PCR (qRT-PCR), immunofluorescence staining and Western blot. The qRT-PCR analysis revealed that OA1 mRNA was expressed in all mice skin samples tested, with the highest expression level in brown skin, a moderate expression level in black skin and the lowest expression level in gray skin. Positive OA1 protein bands were also detected in all skin samples by Western blot analysis. The relative expression levels of OA1 protein in both black and brown skin were significantly higher than that in gray skin, but there was no significant difference between black and brown mice. Immunofluorescence assays revealed that OA1 was mainly expressed in the hair follicle matrix, the inner and outer root sheath in the skin tissues with different coat colors. To get further insight into the important role of OA1 in the melanocytes\u2019 pigmentation, we transfected the OA1 into mouse melanocytes and then detected the relative expression levels of pigmentation-related gene. Simultaneously, we tested the melanin content of melanocytes. As a result, the overexpression of OA1 significantly increased the expression levels of microphthalmia-associated transcription factor (MITF), tyrosinase (TYR), tyrosinase-related protein 1 (TRP1) and premelanosome protein (PMEL). However, the tyrosinase-related protein 2 (TRP2) level was attenuated. By contrast, the level of glycoprotein non-metastatic melanoma protein b (GPNMB) was unaffected by OA1 overexpression. Furthermore, we observed a significant increase in melanin content in mouse melanocyte transfected OA1. Therefore, we propose that OA1 may participate in the formation of coat color by regulating the level of MITF and the number, size, motility and maturation of melanosome.<\/jats:p>","DOI":"10.3390\/ijms17101596","type":"journal-article","created":{"date-parts":[[2016,9,27]],"date-time":"2016-09-27T10:14:11Z","timestamp":1474971251000},"page":"1596","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Ocular Albinism Type 1 Regulates Melanogenesis in Mouse Melanocytes"],"prefix":"10.3390","volume":"17","author":[{"given":"Tianzhi","family":"Chen","sequence":"first","affiliation":[{"name":"College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030600, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haidong","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030600, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Northeast Agricultural University, Harbin 150000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bingling","family":"Zhao","sequence":"additional","affiliation":[{"name":"College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030600, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuanyuan","family":"Zhao","sequence":"additional","affiliation":[{"name":"Wujiang River Institute of Agricultural & Forestry Economic, Tongren University, Tongren 554300, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ruiwen","family":"Fan","sequence":"additional","affiliation":[{"name":"College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030600, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pengchao","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030600, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Changsheng","family":"Dong","sequence":"additional","affiliation":[{"name":"College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030600, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2016,9,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1082","DOI":"10.1038\/1971082a0","article-title":"Chemical composition and terminology of specialized organelles (melanosomes and melanin granules) in mammalian melanocytes","volume":"197","author":"Seiji","year":"1963","journal-title":"Nature"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/j.jdermsci.2004.08.014","article-title":"Biogenesis of pigment granules: A sensitive way to regulate melanocyte function","volume":"37","author":"Hearing","year":"2005","journal-title":"J. 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