{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T15:31:13Z","timestamp":1789659073113,"version":"4.0.1"},"reference-count":41,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2016,10,1]],"date-time":"2016-10-01T00:00:00Z","timestamp":1475280000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>In the present study, IL-1\u03b2 cDNA was identified and analyzed from largemouth bass (Micropterus salmoides). Full length IL-1\u03b2 mRNA was obtained using Rapid Amplification of cDNA Ends (RACE), which contains 78 bp 3\u2032-UTR, a 455 bp 5\u2032-UTR, and an open reading frame (ORF) of 702 bp coding for 233 amino acid residues. The molecular weight and theoretical isoelectric point of largemouth bass IL-1\u03b2 protein was predicted to be 26.7 kDa and 6.08 respectively. A largemouth bass IL-1\u03b2 phylogenetic analysis showed a close relation to the IL-1\u03b2s of striped trumpeter (Latris lineata), Chinese perch (Siniperca chuatsi), and Japanese sea bass (Lateolabrax japonicus). Peptidoglycan upregulated IL-1\u03b2 in the spleen and head kidney, while lipopolysaccharide upregulated detectable levels of IL-1\u03b2 in the spleen only. Largemouth bass, challenged with Nocardia seriolae (1.0 \u00d7 106 cfu\/mL), showed a significant increase in IL-1\u03b2 at 3 and 5 days post infection (dpi) in the spleen, while in the head kidney significant expression was found at 2 and 3 dpi, peaking at 3 dpi. Furthermore, tumor necrosis factor \u03b1 (TNF-\u03b1) showed significantly higher expression in the spleen at 3 and 5 dpi, and in the head kidney at 1 and 3 dpi, with expression decreasing at 5 dpi in both tissues.<\/jats:p>","DOI":"10.3390\/ijms17101670","type":"journal-article","created":{"date-parts":[[2016,10,3]],"date-time":"2016-10-03T10:17:01Z","timestamp":1475489821000},"page":"1670","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":43,"title":["Identification, Molecular Cloning of IL-1\u03b2 and Its Expression Profile during Nocardia seriolae Infection in Largemouth Bass, Micropterus salmoides"],"prefix":"10.3390","volume":"17","author":[{"given":"Ping-Yueh","family":"Ho","sequence":"first","affiliation":[{"name":"Department of Veterinary Medicine, College of Veterinary Medicine, National Pingtung University of Science and Technology, Pingtung 912, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Omkar","family":"Byadgi","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, College of Veterinary Medicine, National Pingtung University of Science and Technology, Pingtung 912, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pei-Chyi","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, College of Veterinary Medicine, National Pingtung University of Science and Technology, Pingtung 912, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming-An","family":"Tsai","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, College of Veterinary Medicine, National Pingtung University of Science and Technology, Pingtung 912, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li-Ling","family":"Liaw","sequence":"additional","affiliation":[{"name":"Bioresource Collection and Research Center, Food Industry Research and Development Institute, Hsinchu 300, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2885-9370","authenticated-orcid":false,"given":"Shih-Chu","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, College of Veterinary Medicine, National Pingtung University of Science and Technology, Pingtung 912, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2016,10,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1096\/fasebj.2.2.3277884","article-title":"Biology of interleukin 1","volume":"2","author":"Dinarello","year":"1988","journal-title":"FASEB J."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"599","DOI":"10.1002\/eji.201040319","article-title":"IL-1: Discoveries, controversies and future directions","volume":"40","author":"Dinarello","year":"2010","journal-title":"Eur. 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