{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,27]],"date-time":"2025-11-27T06:35:53Z","timestamp":1764225353750,"version":"build-2065373602"},"reference-count":30,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2016,12,13]],"date-time":"2016-12-13T00:00:00Z","timestamp":1481587200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Stem cells from human exfoliated deciduous tooth (SHED) offer several advantages over other stem cell sources. Using SHED, we examined the roles of zinc and the zinc uptake transporter ZIP8 (Zrt- and irt-like protein 8) while inducing SHED into insulin secreting \u03b2 cell-like stem cells (i.e., SHED-\u03b2 cells). We observed that ZIP8 expression increased as SHED differentiated into SHED-\u03b2 cells, and that zinc supplementation at day 10 increased the levels of most pancreatic \u03b2 cell markers\u2014particularly Insulin and glucose transporter 2 (GLUT2). We confirmed that SHED-\u03b2 cells produce insulin successfully. In addition, we note that zinc supplementation significantly increases insulin secretion with a significant elevation of ZIP8 transporters in SHED-\u03b2 cells. We conclude that SHED can be converted into insulin-secreting \u03b2 cell-like cells as zinc concentration in the cytosol is elevated. Insulin production by SHED-\u03b2 cells can be regulated via modulation of zinc concentration in the media as ZIP8 expression in the SHED-\u03b2 cells increases.<\/jats:p>","DOI":"10.3390\/ijms17122092","type":"journal-article","created":{"date-parts":[[2016,12,13]],"date-time":"2016-12-13T10:15:52Z","timestamp":1481624152000},"page":"2092","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Zinc Up-Regulates Insulin Secretion from \u03b2 Cell-Like Cells Derived from Stem Cells from Human Exfoliated Deciduous Tooth (SHED)"],"prefix":"10.3390","volume":"17","author":[{"given":"Gyuyoup","family":"Kim","sequence":"first","affiliation":[{"name":"School of Medicine Department of Obstetrics, Gynecology and Reproductive Sciences, University of Maryland, Baltimore, MD 21201, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ki-Hyuk","family":"Shin","sequence":"additional","affiliation":[{"name":"UCLA School of Dentistry, Los Angeles, CA 90095, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eung-Kwon","family":"Pae","sequence":"additional","affiliation":[{"name":"Department of Orthodontics and Pediatric Dentistry, School of Dentistry, University of Maryland, Baltimore, MD 21201, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2016,12,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Vogel, G. 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