{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T22:55:16Z","timestamp":1792709716171,"version":"4.5.0"},"reference-count":48,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2017,2,14]],"date-time":"2017-02-14T00:00:00Z","timestamp":1487030400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Platelet rich plasma (PRP) was tested as a potential therapy for androgenetic alopecia (AGA) through two different clinical protocols in which one population (18 participants) received half-head treatment with autologous non-activated PRP (A-PRP) produced by CPunT Preparation System (Biomed Device, Modena, Italy) and the other half-head with placebo, and a second separated population in which all participants (n = 6, 3 participants per group) received treatment with calcium-activated PRP (AA-PRP) produced from one of two different PRP collection devices (Regen Blood Cell Therapy or Arthrex Angel System). For the A-PRP study, three treatments were administered over 30-day intervals. Trichoscan analysis of patients, three months post-treatment, showed a clinical improvement in the number of hairs in the target area (36 \u00b1 3 hairs) and in total hair density (65\u00b1  5 hair cm2), whereas negligible improvements in hair count (1.1\u00b1  1.4 hairs) and density (1.9 \u00b1 10.2 hair cm2) were seen in the region of the scalp that received placebo. Microscopic evaluation conducted two weeks after treatment showed also an increase in epidermal thickness, Ki67+ keratinocytes, and in the number of follicles. The AA-PRP treatment groups received a singular set of injections, and six months after the treatments were administered, notable differences in clinical outcomes were obtained from the two PRP collection devices (+90 \u00b1 6 hair cm2 versus -73 \u00b1 30 hair cm2 hair densities, Regen versus Arthrex). Growth factor concentrations in AA-PRP prepared from the two collection devices did not differ significantly upon calcium activation.<\/jats:p>","DOI":"10.3390\/ijms18020408","type":"journal-article","created":{"date-parts":[[2017,2,15]],"date-time":"2017-02-15T10:09:07Z","timestamp":1487153347000},"page":"408","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":170,"title":["Evaluation of Not-Activated and Activated PRP in Hair Loss Treatment: Role of Growth Factor and Cytokine Concentrations Obtained by Different Collection Systems"],"prefix":"10.3390","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3123-3977","authenticated-orcid":false,"given":"Pietro","family":"Gentile","sequence":"first","affiliation":[{"name":"Plastic and Reconstructive Surgery Department, University of Rome Tor Vergata, Via Courmayeur, No. 102, 00135 Rome, Italy"},{"name":"Plastic and Reconstructive Surgery Department, Catholic University, 1005 Tiranna, Albania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John","family":"Cole","sequence":"additional","affiliation":[{"name":"Cole Hair Transplant Group, Alpharetta, 30004 GA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Megan","family":"Cole","sequence":"additional","affiliation":[{"name":"Cole Hair Transplant Group, Alpharetta, 30004 GA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simone","family":"Garcovich","sequence":"additional","affiliation":[{"name":"Institute of Dermatology, Catholic University of the Sacred Heart, 00168 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandra","family":"Bielli","sequence":"additional","affiliation":[{"name":"Institute of Anatomic Pathology, University of Rome Tor Vergata, 00133 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Scioli","sequence":"additional","affiliation":[{"name":"Institute of Anatomic Pathology, University of Rome Tor Vergata, 00133 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Augusto","family":"Orlandi","sequence":"additional","affiliation":[{"name":"Institute of Anatomic Pathology, University of Rome Tor Vergata, 00133 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chiara","family":"Insalaco","sequence":"additional","affiliation":[{"name":"Plastic and Reconstructive Surgery Department, University of Rome Tor Vergata, Via Courmayeur, No. 102, 00135 Rome, Italy"},{"name":"Cole Hair Transplant Group, Alpharetta, 30004 GA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valerio","family":"Cervelli","sequence":"additional","affiliation":[{"name":"Plastic and Reconstructive Surgery Department, University of Rome Tor Vergata, Via Courmayeur, No. 102, 00135 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2017,2,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1017\/S1462399402005112","article-title":"Androgenetic alopecia: Pathogenesis and potential for therapy","volume":"4","author":"Ellis","year":"2002","journal-title":"Expert Rev. 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