{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T23:21:19Z","timestamp":1791328879975,"version":"4.3.1"},"reference-count":62,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2017,2,21]],"date-time":"2017-02-21T00:00:00Z","timestamp":1487635200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Sex differences have been identified in various biological processes, including hypertension. The mineralocorticoid signaling pathway is an important contributor to early arterial hypertension, however its sex-specific expression has been scarcely studied, particularly with respect to the kidney. Basal systolic blood pressure (SBP) and heart rate (HR) were measured in adult male and female mice. Renal gene expression studies of major players of mineralocorticoid signaling were performed at different developmental stages in male and female mice using reverse transcription quantitative PCR (RT-qPCR), and were compared to those of the same genes in the lung, another mineralocorticoid epithelial target tissue that regulates ion exchange and electrolyte balance. The role of sex hormones in the regulation of these genes was also investigated in differentiated KC3AC1 renal cells. Additionally, renal expression of the 11 \u03b2-hydroxysteroid dehydrogenase type 2 (11\u03b2HSD2) protein, a regulator of mineralocorticoid specificity, was measured by immunoblotting and its activity was indirectly assessed in the plasma using liquid-chromatography coupled to mass spectrometry in tandem (LC-MSMS) method. SBP and HR were found to be significantly lower in females compared to males. This was accompanied by a sex- and tissue-specific expression profile throughout renal development of the mineralocorticoid target genes serum and glucocorticoid-regulated kinase 1 (Sgk1) and glucocorticoid-induced leucine zipper protein (Gilz), together with Hsd11b2, Finally, the implication of sex hormones in this sex-specific expression profile was demonstrated in vitro, most notably for Gilz mRNA expression. We demonstrate a tissue-specific, sex-dependent and developmentally-regulated pattern of expression of the mineralocorticoid pathway that could have important implications in physiology and pathology.<\/jats:p>","DOI":"10.3390\/ijms18020457","type":"journal-article","created":{"date-parts":[[2017,2,22]],"date-time":"2017-02-22T11:40:52Z","timestamp":1487763652000},"page":"457","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Sex-Specificity of Mineralocorticoid Target Gene Expression during Renal Development, and Long-Term Consequences"],"prefix":"10.3390","volume":"18","author":[{"given":"Laurence","family":"Dumeige","sequence":"first","affiliation":[{"name":"Inserm U1185, Univ Paris Sud, Universit\u00e9 Paris-Saclay, F-94276 Le Kremlin-Bic\u00eatre, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caroline","family":"Storey","sequence":"additional","affiliation":[{"name":"Inserm U1185, Univ Paris Sud, Universit\u00e9 Paris-Saclay, F-94276 Le Kremlin-Bic\u00eatre, France"},{"name":"Service d\u2019Endocrinologie P\u00e9diatrique, H\u00f4pital Robert Debr\u00e9, Assistance Publique H\u00f4pitaux de Paris, F-75019 Paris, France"},{"name":"Facult\u00e9 de M\u00e9decine Paris Diderot, Universit\u00e9 Paris Diderot, Sorbonne Paris Cit\u00e9, F-75019 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lyvianne","family":"Decourtye","sequence":"additional","affiliation":[{"name":"Inserm, UMR_S938, Centre de Recherche Saint-Antoine, F-75012 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melanie","family":"Nehlich","sequence":"additional","affiliation":[{"name":"Inserm U1185, Univ Paris Sud, Universit\u00e9 Paris-Saclay, F-94276 Le Kremlin-Bic\u00eatre, France"},{"name":"PremUp Foundation, F-75005 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christophe","family":"Lhadj","sequence":"additional","affiliation":[{"name":"Inserm U1185, Univ Paris Sud, Universit\u00e9 Paris-Saclay, F-94276 Le Kremlin-Bic\u00eatre, France"},{"name":"PremUp Foundation, F-75005 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Say","family":"Viengchareun","sequence":"additional","affiliation":[{"name":"Inserm U1185, Univ Paris Sud, Universit\u00e9 Paris-Saclay, F-94276 Le Kremlin-Bic\u00eatre, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurent","family":"Kappeler","sequence":"additional","affiliation":[{"name":"Inserm, UMR_S938, Centre de Recherche Saint-Antoine, F-75012 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marc","family":"Lomb\u00e8s","sequence":"additional","affiliation":[{"name":"Inserm U1185, Univ Paris Sud, Universit\u00e9 Paris-Saclay, F-94276 Le Kremlin-Bic\u00eatre, France"},{"name":"PremUp Foundation, F-75005 Paris, France"},{"name":"Facult\u00e9 de M\u00e9decine Paris-Sud, UMR-S1185, Universit\u00e9 Paris-Sud 11, F-94276 Le Kremlin-Bic\u00eatre, France"},{"name":"Service d\u2019Endocrinologie et Maladies de la Reproduction, H\u00f4pital de Bic\u00eatre, Assistance Publique-H\u00f4pitaux de Paris, F-94275 Le Kremlin Bic\u00eatre, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laetitia","family":"Martinerie","sequence":"additional","affiliation":[{"name":"Inserm U1185, Univ Paris Sud, Universit\u00e9 Paris-Saclay, F-94276 Le Kremlin-Bic\u00eatre, France"},{"name":"Service d\u2019Endocrinologie P\u00e9diatrique, H\u00f4pital Robert Debr\u00e9, Assistance Publique H\u00f4pitaux de Paris, F-75019 Paris, France"},{"name":"Facult\u00e9 de M\u00e9decine Paris Diderot, Universit\u00e9 Paris Diderot, Sorbonne Paris Cit\u00e9, F-75019 Paris, France"},{"name":"PremUp Foundation, F-75005 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2017,2,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"820","DOI":"10.1016\/j.neuroimage.2013.09.040","article-title":"Why size matters: Differences in brain volume account for apparent sex differences in callosal anatomy: The sexual dimorphism of the corpus callosum","volume":"84","author":"Luders","year":"2014","journal-title":"Neuroimage"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1016\/j.bbr.2015.02.009","article-title":"Sex differences in the stress response in sd rats","volume":"284","author":"Lu","year":"2015","journal-title":"Behav. 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