{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T14:59:37Z","timestamp":1791644377507,"version":"4.3.4"},"reference-count":47,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2017,3,30]],"date-time":"2017-03-30T00:00:00Z","timestamp":1490832000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Centella asiatica has potent antioxidant and anti-inflammatory properties. However, its anti-dermatitic effect has not yet been reported. In this study, we investigated the anti-dermatitic effects of titrated extract of Centella asiatica (TECA) in a phthalic anhydride (PA)-induced atopic dermatitis (AD) animal model as well as in vitro model. An AD-like lesion was induced by the topical application of five percent PA to the dorsal skin or ear of Hos:HR-1 mouse. After AD induction, 100 \u03bcL of 0.2% and 0.4% of TECA (40 \u03bcg or 80 \u03bcg\/cm2) was spread on the dorsum of the ear or back skin three times a week for four weeks. We evaluated dermatitis severity, histopathological changes and changes in protein expression by Western blotting for inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and NF-\u03baB activity, which were determined by electromobility shift assay (EMSA). We also measured TNF-\u03b1, IL-1\u03b2, IL-6, and IgE concentration in the blood of AD mice by enzyme-linked immunosorbent assay (ELISA). TECA treatment attenuated the development of PA-induced atopic dermatitis. Histological analysis showed that TECA inhibited hyperkeratosis, mast cells and infiltration of inflammatory cells. TECA treatment inhibited expression of iNOS and COX-2, and NF-\u03baB activity as well as the release of TNF-\u03b1, IL-1\u03b2, IL-6, and IgE. In addition, TECA (1, 2, 5 \u03bcg\/mL) potently inhibited Lipopolysaccharide (LPS) (1 \u03bcg\/mL)-induced NO production, expression of iNOS and COX-2, and NF-\u03baB DNA binding activities in RAW264.7 macrophage cells. Our data demonstrated that TECA could be a promising agent for AD by inhibition of NF-\u03baB signaling.<\/jats:p>","DOI":"10.3390\/ijms18040738","type":"journal-article","created":{"date-parts":[[2017,3,30]],"date-time":"2017-03-30T09:49:54Z","timestamp":1490867394000},"page":"738","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":86,"title":["Anti-Inflammatory Effect of Titrated Extract of Centella asiatica in Phthalic Anhydride-Induced Allergic Dermatitis Animal Model"],"prefix":"10.3390","volume":"18","author":[{"given":"Ju","family":"Park","sequence":"first","affiliation":[{"name":"College of Pharmacy and Medical Research Center, Chungbuk National University, 194-31 Osongsaengmyeong 1-ro, Osong-eup, Heungduk-gu, Cheongju 361-951, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ji","family":"Choi","sequence":"additional","affiliation":[{"name":"College of Pharmacy and Medical Research Center, Chungbuk National University, 194-31 Osongsaengmyeong 1-ro, Osong-eup, Heungduk-gu, Cheongju 361-951, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7520-6909","authenticated-orcid":false,"given":"Dong","family":"Son","sequence":"additional","affiliation":[{"name":"College of Pharmacy and Medical Research Center, Chungbuk National University, 194-31 Osongsaengmyeong 1-ro, Osong-eup, Heungduk-gu, Cheongju 361-951, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0203-6731","authenticated-orcid":false,"given":"Eun","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Obstetrics & Gynecology, Daejeon St. Mary\u2019s Hospital, The Catholic University of Korea, 64 Daeheung-Ro (Daeheung-dong), Jung-gu, Daejeon 301-723, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Song","sequence":"additional","affiliation":[{"name":"Department of Obstetrics & Gynecology, Daejeon St. Mary\u2019s Hospital, The Catholic University of Korea, 64 Daeheung-Ro (Daeheung-dong), Jung-gu, Daejeon 301-723, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mats","family":"Hellstr\u00f6m","sequence":"additional","affiliation":[{"name":"Laboratory for Transplantation and Regenerative Medicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg 411-15, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6534-9575","authenticated-orcid":false,"given":"Jin","family":"Hong","sequence":"additional","affiliation":[{"name":"College of Pharmacy and Medical Research Center, Chungbuk National University, 194-31 Osongsaengmyeong 1-ro, Osong-eup, Heungduk-gu, Cheongju 361-951, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2017,3,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2609","DOI":"10.1002\/eji.201646421","article-title":"Increased Th2 activity and diminished skin barrier function cooperate in allergic skin inflammation","volume":"46","author":"Sehra","year":"2016","journal-title":"Eur. 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