{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T04:11:45Z","timestamp":1760242305369,"version":"build-2065373602"},"reference-count":49,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2017,4,5]],"date-time":"2017-04-05T00:00:00Z","timestamp":1491350400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Charcot-Marie-Tooth disease (CMT) is a common inherited peripheral neuropathy affecting up to 1 in 1214 of the general population with more than 60 nuclear genes implicated in its pathogenesis. Traditional molecular diagnostic pathways based on relative prevalence and clinical phenotyping are limited by long turnaround time, population-specific prevalence of causative variants and inability to assess multiple co-existing variants. In this study, a CMT gene panel comprising 27 genes was used to uncover the pathogenic mutations in two index patients. The first patient is a 15-year-old boy, born of consanguineous parents, who has had frequent trips and falls since infancy, and was later found to have inverted champagne bottle appearance of bilateral legs and foot drop. His elder sister is similarly affected. The second patient is a 37-year-old woman referred for pre-pregnancy genetic diagnosis. During early adulthood, she developed progressive lower limb weakness, difficulties in tip-toe walking and thinning of calf muscles. Both patients are clinically compatible with CMT, have undergone multiple genetic testings and have not previously received a definitive genetic diagnosis. Patients 1 and 2 were found to have pathogenic homozygous HSPB1:NM_001540:c.250G&gt;A (p.G84R) variant and heterozygous GDAP1:NM_018972:c.358C&gt;T (p.R120W) variant, respectively. Advantages and limitations of the current approach are discussed.<\/jats:p>","DOI":"10.3390\/ijms18040770","type":"journal-article","created":{"date-parts":[[2017,4,5]],"date-time":"2017-04-05T10:33:01Z","timestamp":1491388381000},"page":"770","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Rapid Identification of Pathogenic Variants in Two Cases of Charcot-Marie-Tooth Disease by Gene-Panel Sequencing"],"prefix":"10.3390","volume":"18","author":[{"given":"Chi-Chun","family":"Ho","sequence":"first","affiliation":[{"name":"Department of Clinical Pathology, Pamela Youde Nethersole Eastern Hospital, Chai Wan, Hong Kong, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuk-Mui","family":"Tai","sequence":"additional","affiliation":[{"name":"Department of Paediatrics &amp; Adolescent Medicine, Pamela Youde Nethersole Eastern Hospital, Chai Wan, Hong Kong, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edmond","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Medicine, Pamela Youde Nethersole Eastern Hospital, Chai Wan, Hong Kong, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Timothy","family":"Mak","sequence":"additional","affiliation":[{"name":"Centre for Genomic Sciences, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Timothy","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Paediatrics &amp; Adolescent Medicine, Pamela Youde Nethersole Eastern Hospital, Chai Wan, Hong Kong, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Victor","family":"Tang","sequence":"additional","affiliation":[{"name":"Department of Clinical Pathology, Pamela Youde Nethersole Eastern Hospital, Chai Wan, Hong Kong, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wing-Tat","family":"Poon","sequence":"additional","affiliation":[{"name":"Department of Clinical Pathology, Pamela Youde Nethersole Eastern Hospital, Chai Wan, Hong Kong, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2017,4,5]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"iv-22","DOI":"10.1111\/ane.12013","article-title":"Genetic epidemiology of Charcot-Marie-Tooth disease","volume":"126","author":"Braathen","year":"2012","journal-title":"Acta Neurol. 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