{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T01:16:05Z","timestamp":1792804565125,"version":"4.5.0"},"reference-count":62,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2017,4,19]],"date-time":"2017-04-19T00:00:00Z","timestamp":1492560000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministry of Education, Science, and Culture of the State of Mecklenburg-Western Pomerania","award":["AU 11 038"],"award-info":[{"award-number":["AU 11 038"]}],"id":[{"id":"https:\/\/ror.org\/00949d647","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Ministry of Education, Science, and Culture of the State of Mecklenburg-Western Pomerania","award":["ESF\/IV-BM-B35-0010\/13"],"award-info":[{"award-number":["ESF\/IV-BM-B35-0010\/13"]}],"id":[{"id":"https:\/\/ror.org\/00949d647","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Ministry of Education, Science, and Culture of the State of Mecklenburg-Western Pomerania","award":["AU 15 001"],"award-info":[{"award-number":["AU 15 001"]}],"id":[{"id":"https:\/\/ror.org\/00949d647","id-type":"ROR","asserted-by":"crossref"}]},{"name":"German Federal Ministry of Eduation and research","award":["03Z22DN11"],"award-info":[{"award-number":["03Z22DN11"]}],"id":[{"id":"https:\/\/ror.org\/04pz7b180","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Multiple evidence in animal models and in humans suggest a beneficial role of cold physical plasma in wound treatment. Yet, risk assessment studies are important to further foster therapeutic advancement and acceptance of cold plasma in clinics. Accordingly, we investigated the longterm side effects of repetitive plasma treatment over 14 consecutive days in a rodent full-thickness ear wound model. Subsequently, animals were housed for 350 days and sacrificed thereafter. In blood, systemic changes of the proinflammatory cytokines interleukin 1\u03b2 and tumor necrosis factor \u03b1 were absent. Similarly, tumor marker levels of \u03b1-fetoprotein and calcitonin remained unchanged. Using quantitative PCR, the expression levels of several cytokines and tumor markers in liver, lung, and skin were found to be similar in the control and treatment group as well. Likewise, histological and immunohistochemical analysis failed to detect abnormal morphological changes and the presence of tumor markers such as carcinoembryonic antigen, \u03b1-fetoprotein, or the neighbor of Punc 11. Absence of neoplastic lesions was confirmed by non-invasive imaging methods such as anatomical magnetic resonance imaging and positron emission tomography-computed tomography. Our results suggest that the beneficial effects of cold plasma in wound healing come without apparent side effects including tumor formation or chronic inflammation.<\/jats:p>","DOI":"10.3390\/ijms18040868","type":"journal-article","created":{"date-parts":[[2017,4,19]],"date-time":"2017-04-19T10:22:01Z","timestamp":1492597321000},"page":"868","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":101,"title":["One Year Follow-Up Risk Assessment in SKH-1 Mice and Wounds Treated with an Argon Plasma Jet"],"prefix":"10.3390","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6488-3904","authenticated-orcid":false,"given":"Anke","family":"Schmidt","sequence":"first","affiliation":[{"name":"Leibniz-Institute for Plasma Science and Technology (INP Greifswald), Departments of Plasma Life Science and ZIK Plasmatis, Felix-Hausdorff-Str. 2, 17489 Greifswald, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1097-4832","authenticated-orcid":false,"given":"Thomas","family":"Woedtke","sequence":"additional","affiliation":[{"name":"Leibniz-Institute for Plasma Science and Technology (INP Greifswald), Departments of Plasma Life Science and ZIK Plasmatis, Felix-Hausdorff-Str. 2, 17489 Greifswald, Germany"},{"name":"Department of Hygiene and Environmental Medicine, University Medicine Greifswald, 17475 Greifswald, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Stenzel","sequence":"additional","affiliation":[{"name":"Core Facility Multimodal Small Animal Imaging, 18057 Rostock, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7826-3132","authenticated-orcid":false,"given":"Tobias","family":"Lindner","sequence":"additional","affiliation":[{"name":"Core Facility Multimodal Small Animal Imaging, 18057 Rostock, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan","family":"Polei","sequence":"additional","affiliation":[{"name":"Core Facility Multimodal Small Animal Imaging, 18057 Rostock, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brigitte","family":"Vollmar","sequence":"additional","affiliation":[{"name":"Institute for Experimental Surgery, Rostock University Medical Center, Schillingallee 69a, 18057 Rostock, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8773-8862","authenticated-orcid":false,"given":"Sander","family":"Bekeschus","sequence":"additional","affiliation":[{"name":"Leibniz-Institute for Plasma Science and Technology (INP Greifswald), Departments of Plasma Life Science and ZIK Plasmatis, Felix-Hausdorff-Str. 2, 17489 Greifswald, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2017,4,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"014031","DOI":"10.1088\/0741-3335\/59\/1\/014031","article-title":"Plasma medicine-current state of research and medical application","volume":"59","author":"Weltmann","year":"2017","journal-title":"Plasma Phys. 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