{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,13]],"date-time":"2026-04-13T15:43:23Z","timestamp":1776095003139,"version":"3.50.1"},"reference-count":58,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2017,6,15]],"date-time":"2017-06-15T00:00:00Z","timestamp":1497484800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["03\/10424-0"],"award-info":[{"award-number":["03\/10424-0"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["05\/03231-7"],"award-info":[{"award-number":["05\/03231-7"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>This study aimed to investigate whether the \u22121026(A&gt;C)(rs2779249) and +2087(A&gt;G)(2297518) polymorphisms in the NOS2 gene were associated with chronic periodontitis (CP) and with salivary levels of nitrite (NO2\u2212) and\/or nitrate + nitrite (NOx). A group of 113 mixed-race patients were subjected to periodontal, genetic, and biochemical evaluations (65 CP\/48 periodontally healthy subjects). DNA was extracted from oral epithelial cells and used for genotyping by polymerase chain reaction (real-time). Salivary NOx concentrations were determined using an ozone-based chemiluminescence assay. Association of CP with alleles and genotypes of the \u22121026(A&gt;C) polymorphism was found (X2 test, p = 0.0075; 0.0308), but this was not maintained after multiple logistic regression, performed to estimate the effect of covariates and polymorphisms in CP. This analysis demonstrated, after correction for multiple comparisons, that only the female gender was significantly associated with CP. Polymorphisms analyzed as haplotypes were not associated with CP. NOx levels were significantly higher in the control group of heterozygous individuals for both polymorphisms. In conclusion, the female gender was significantly associated with CP, and higher levels of salivary NOx were found in control subjects and associated with the heterozygous state of the NOS2 polymorphisms, reinforcing the potential of NO metabolites as markers of periodontitis status.<\/jats:p>","DOI":"10.3390\/ijms18061128","type":"journal-article","created":{"date-parts":[[2017,6,15]],"date-time":"2017-06-15T10:07:33Z","timestamp":1497521253000},"page":"1128","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Inducible Nitric Oxide Synthase Polymorphisms and Nitric Oxide Levels in Individuals with Chronic Periodontitis"],"prefix":"10.3390","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5068-0268","authenticated-orcid":false,"given":"Raquel","family":"Scarel-Caminaga","sequence":"first","affiliation":[{"name":"Department of Morphology, School of Dentistry at Araraquara, S\u00e3o Paulo State University (UNESP), Humaita St., 1680, Araraquara CEP 14801-903, S\u00e3o Paulo, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fl\u00e1via","family":"Cera","sequence":"additional","affiliation":[{"name":"Department of Morphology, School of Dentistry at Araraquara, S\u00e3o Paulo State University (UNESP), Humaita St., 1680, Araraquara CEP 14801-903, S\u00e3o Paulo, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7067-6119","authenticated-orcid":false,"given":"Suzane","family":"Pigossi","sequence":"additional","affiliation":[{"name":"Department of Diagnosis and Surgery, School of Dentistry at Araraquara, S\u00e3o Paulo State University (UNESP), Humaita St., 1680, Araraquara CEP 14801-903, S\u00e3o Paulo, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Livia","family":"Finoti","sequence":"additional","affiliation":[{"name":"Department of Periodontics, School of Dental Medicine, University of Pennsylvania, 240 S 40th St., Philadelphia, PA 19104, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yeon","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Implantology, University of Santo Amaro, Professor En\u00e9as de Siqueira Neto, St., 340, Santo Amaro CEP 04829-300, S\u00e3o Paulo, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aline","family":"Viana","sequence":"additional","affiliation":[{"name":"Department of Diagnosis and Surgery, School of Dentistry at Araraquara, S\u00e3o Paulo State University (UNESP), Humaita St., 1680, Araraquara CEP 14801-903, S\u00e3o Paulo, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rodrigo","family":"Secolin","sequence":"additional","affiliation":[{"name":"Department of Medical Genetics, University of Campinas\u2014UNICAMP, Tess\u00e1lia Vieira de Camargo St., 126 Campinas CEP 13083-887, S\u00e3o Paulo, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcelo","family":"Montenegro","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Ribeir\u00e3o Preto Medical School, University of S\u00e3o Paulo, Bandeirantes Avenue, 3900, Ribeir\u00e3o Preto CEP 14.049-900, S\u00e3o Paulo, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9","family":"Tanus-Santos","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Ribeir\u00e3o Preto Medical School, University of S\u00e3o Paulo, Bandeirantes Avenue, 3900, Ribeir\u00e3o Preto CEP 14.049-900, S\u00e3o Paulo, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Silvana","family":"Orrico","sequence":"additional","affiliation":[{"name":"Department of Diagnosis and Surgery, School of Dentistry at Araraquara, S\u00e3o Paulo State University (UNESP), Humaita St., 1680, Araraquara CEP 14801-903, S\u00e3o Paulo, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joni","family":"Cirelli","sequence":"additional","affiliation":[{"name":"Department of Diagnosis and Surgery, School of Dentistry at Araraquara, S\u00e3o Paulo State University (UNESP), Humaita St., 1680, Araraquara CEP 14801-903, S\u00e3o Paulo, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2017,6,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"American Academy of Periodontology (2000). 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