{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T14:30:20Z","timestamp":1779892220199,"version":"3.53.1"},"reference-count":27,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2017,11,26]],"date-time":"2017-11-26T00:00:00Z","timestamp":1511654400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Key R&amp;D Program of China","award":["2016YFD0501210, 2017YFD0500505"],"award-info":[{"award-number":["2016YFD0501210, 2017YFD0500505"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31372319, 31572416, 31402084"],"award-info":[{"award-number":["31372319, 31572416, 31402084"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Hubei Provincial Technology and Innovation Program","award":["2016ABA121,2017AHB062"],"award-info":[{"award-number":["2016ABA121,2017AHB062"]}]},{"DOI":"10.13039\/501100003819","name":"Natural Science Foundation of Hubei Province","doi-asserted-by":"publisher","award":["2016CFA070"],"award-info":[{"award-number":["2016CFA070"]}],"id":[{"id":"10.13039\/501100003819","id-type":"DOI","asserted-by":"publisher"}]},{"name":"he Hubei Hundred Talent Program, Agriculture and Food Research Initiative Competitive Grants of (2014-67015-21770) of the USDA National Institute of Food and Agriculture, and Texas AgriLife Research","award":["H-82000"],"award-info":[{"award-number":["H-82000"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>This study aims to determine whether Lactobacillus casei (L. casei) could relieve liver injury in piglets challenged with lipopolysaccharide (LPS). Piglets were randomly allocated into one of the three groups: control, LPS, and L. casei. The control and LPS groups were fed a corn- and soybean meal-based diet, whereas the L. casei group was fed the basal diet supplemented with 6 \u00d7 106 cfu\/g L. casei. On Day 31 of the trial, piglets in the LPS and L. casei groups received intraperitoneal administration of LPS (100 \u00b5g\/kg body weight), while the control group received the same volume of saline. Blood and liver samples were collected for analysis. Results showed that L. casei supplementation decreased the feed\/gain ratio (p = 0.027) and diarrhea incidence (p &lt; 0.001), and attenuated LPS-induced liver histomorphological abnormalities. Compared with the control group, LPS challenge dramatically increased glutamyl transpeptidase activity (p = 0.001) in plasma as well as the concentrations of Interleukin 6 (IL-6) (p = 0.048), Tumor necrosis factor-alpha (TNF-\u03b1) (p = 0.041), and Malondialdehyde (MDA) (p = 0.001) in the liver, while decreasing the hepatic SOD activity. LPS also increased (p &lt; 0.05) the mRNA levels for IL-6, IL-8, TNF-\u03b1, Toll-like receptors 4 (TLR4), Nuclear factor \u03baB (NF-\u03baB) and Heat shock protein 70 (HSP70) in the liver. The adverse effects of LPS challenge were ameliorated by L. casei supplementation. In conclusion, dietary L. casei alleviates LPS-induced liver injury via reducing pro-inflammatory cytokines and increasing anti-oxidative capacity.<\/jats:p>","DOI":"10.3390\/ijms18122535","type":"journal-article","created":{"date-parts":[[2017,11,27]],"date-time":"2017-11-27T11:07:08Z","timestamp":1511780828000},"page":"2535","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Dietary Supplementation with Lactobacillus casei Alleviates Lipopolysaccharide-Induced Liver Injury in a Porcine Model"],"prefix":"10.3390","volume":"18","author":[{"given":"Di","family":"Zhao","sequence":"first","affiliation":[{"name":"Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Wu","sequence":"additional","affiliation":[{"name":"Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dan","family":"Yi","sequence":"additional","affiliation":[{"name":"Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Wang","sequence":"additional","affiliation":[{"name":"Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peng","family":"Li","sequence":"additional","affiliation":[{"name":"Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junmei","family":"Zhang","sequence":"additional","affiliation":[{"name":"Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongqing","family":"Hou","sequence":"additional","affiliation":[{"name":"Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guoyao","family":"Wu","sequence":"additional","affiliation":[{"name":"Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China"},{"name":"Department of Animal Science, Texas A &amp; M University, College Station, TX 77843, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2017,11,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1251","DOI":"10.1002\/hep.1840190525","article-title":"The fate of intravenously injected endotoxin in normal rats and in rats with liver failure","volume":"19","author":"Nakao","year":"1994","journal-title":"Hepatology"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1560","DOI":"10.1093\/jn\/131.5.1560","article-title":"Green tea suppresses lipopolysaccharide-induced liver injury in d-galactosamine sensitized rats","volume":"131","author":"He","year":"2001","journal-title":"J. Nutr."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1002\/hep.20282","article-title":"Adiponectin protects LPS-Induced liver injury through modulation of TNF-\u03b1 in KK-Ay obese mice","volume":"40","author":"Masaki","year":"2004","journal-title":"Hepatology"},{"key":"ref_4","first-page":"253","article-title":"Role of probiotics in health and disease: A review","volume":"63","author":"Singh","year":"2013","journal-title":"J. Pak. Med. Assoc."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1016\/j.ijfoodmicro.2011.07.008","article-title":"Selective and differential enumerations of Lactobacillus delbrueckii subsp. bulgaricus, Streptococcus thermophilus, Lactobacillus acidophilus, Lactobacillus casei and Bifidobacterium spp. in yoghurt\u2014A review","volume":"149","author":"Ashraf","year":"2011","journal-title":"Int. J. Food Microbiol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"555","DOI":"10.1007\/s00726-010-0473-y","article-title":"Dietary \u03b1-ketoglutarate supplementation ameliorates intestinal injury in lipopolysaccharide-challenged piglets","volume":"39","author":"Hou","year":"2010","journal-title":"Amino Acids"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1159","DOI":"10.1007\/s00726-010-0740-y","article-title":"Composition of amino acids in feed ingredients for animal diets","volume":"40","author":"Li","year":"2011","journal-title":"Amino Acids"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1017\/S0007114513002171","article-title":"Dietary N-acetylcysteine supplementation alleviates liver injury in lipopolysaccharide-challenged piglets","volume":"111","author":"Yi","year":"2014","journal-title":"Br. J. Nutr."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"804","DOI":"10.1177\/1753425912441955","article-title":"Dietary l-arginine supplementation alleviates liver injury caused by Escherichia coli LPS in weaned pigs","volume":"18","author":"Li","year":"2012","journal-title":"Innate Immun."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"504","DOI":"10.1177\/1753425912472003","article-title":"Fish oil attenuates liver injury caused by LPS in weaned pigs associated with inhibition of TLR4 and nucleotide-binding oligomerization domain protein signaling pathways","volume":"19","author":"Chen","year":"2013","journal-title":"Innate Immun."},{"key":"ref_11","first-page":"82","article-title":"Effect of different types of probiotics on intestinal microflora, pH and volatile base nitrogen of weaned piglets","volume":"40","author":"Dou","year":"2013","journal-title":"China Anim. Husb. Vet. Med."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Zakostelska, Z., Kverka, M., Klimesova, K., Rossmann, P., Mrazek, J., Kopecny, J., Hornova, M., Srutkova, D., Hudcovic, T., and Ridl, J. (2011). Lysate of probiotic Lactobacillus casei DN-114 001 ameliorates colitis by strengthening the gutbarrier function and changing the gut microenvironment. PLoS ONE, 6.","DOI":"10.1371\/journal.pone.0027961"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"821","DOI":"10.1007\/s00394-015-0904-3","article-title":"Probiotic Lactobacillus casei Zhang reduces pro-inflammatory cytokine production and hepatic inflammation in a rat model of acute liver failure","volume":"55","author":"Wang","year":"2016","journal-title":"Eur. J. Nutr."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"531","DOI":"10.1016\/j.jnutbio.2012.01.014","article-title":"Lactobacillus casei Shirota protects from fructose-induced liver steatosis: A mouse model","volume":"24","author":"Wagnerberger","year":"2013","journal-title":"J. Nutr. Biochem."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1152\/physrev.1994.74.1.139","article-title":"Cellular defenses against damage from reactive oxygen species","volume":"74","author":"Yu","year":"1994","journal-title":"Physiol. Rev."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1159\/000337585","article-title":"Reduced hepatic injury in Toll-like receptor 4-deficient mice following d-galactosamine\/lipopolysaccharide-induced fulminant hepatic failure","volume":"29","author":"Ben","year":"2012","journal-title":"Cell. Physiol. Biochem."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1111\/imm.12305","article-title":"Wogonin prevents lipopolysaccharide-induced acute lung injury and inflammation in mice via peroxisome proliferator-activated receptor gamma-mediated attenuation of the nuclear factor-\u03baB pathway","volume":"143","author":"Yao","year":"2014","journal-title":"Immunology"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1016\/S0171-2985(11)80342-6","article-title":"Nuclear factor \u03baB, a mediator of lipopolysaccharide effects","volume":"187","author":"Muller","year":"1993","journal-title":"Immunobiology"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1016\/j.intimp.2012.10.026","article-title":"Protective effects of probiotic Lactobacillus casei Zhang against endotoxin- and d-galactosamine-induced liver injury in rats via anti-oxidative and anti-inflammatory capacities","volume":"15","author":"Wang","year":"2013","journal-title":"Int. Immunopharmacol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"513","DOI":"10.1007\/s00726-012-1295-x","article-title":"N-acetylcyseine reduces inflammation in the small intestine by regulating redox, EGF and TLR4 signaling","volume":"45","author":"Hou","year":"2013","journal-title":"Amino Acids"},{"key":"ref_21","unstructured":"Henneberg, W., and Stohmann, F. (1864). Beitr\u00e4ge zur Begr\u00fcndungeiner Rationellen F\u00fc Tterung der Wiederk\u00e4uer, [Contributions to Establish a Rational Feeding of Ruminants], Schwetschtke u. Sohn. [2nd ed.]."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1016\/j.anifeedsci.2016.07.014","article-title":"N-acetylcysteine improves the growth performance andintestinal function in the heat-stressed broilers","volume":"220","author":"Yi","year":"2016","journal-title":"Anim. Feed Sci. Technol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1233","DOI":"10.1007\/s00726-011-1191-9","article-title":"Protective effects of N-acetylcysteine on intestinal functions of piglets challenged with lipopolysaccharide","volume":"43","author":"Hou","year":"2012","journal-title":"Amino Acids"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"487","DOI":"10.2741\/4404","article-title":"Gene expression profiles in the intestine of lipopolysaccharide-challenged piglets","volume":"21","author":"Yi","year":"2016","journal-title":"Front. Biosci."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and 2\u2212\u0394\u0394Ct method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"2031","DOI":"10.1007\/s00726-011-0924-0","article-title":"Comparisons of treatment means when factors do not interact in two-factorial studies","volume":"42","author":"Wei","year":"2012","journal-title":"Amino Acids"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"561","DOI":"10.1016\/j.jnutbio.2009.11.007","article-title":"Statistics and bioinformatics in nutritional sciences: Analysis of complex data in the era of systems biology","volume":"21","author":"Fu","year":"2010","journal-title":"J. Nutr. Biochem."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/18\/12\/2535\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T18:51:23Z","timestamp":1760208683000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/18\/12\/2535"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,11,26]]},"references-count":27,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2017,12]]}},"alternative-id":["ijms18122535"],"URL":"https:\/\/doi.org\/10.3390\/ijms18122535","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,11,26]]}}}