{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T23:37:09Z","timestamp":1790552229845,"version":"4.1.0"},"reference-count":41,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2017,12,8]],"date-time":"2017-12-08T00:00:00Z","timestamp":1512691200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Recent studies have shown promising results concerning the effectiveness of 3D plates in terms of stabilization of condylar fractures. Despite the use of new techniques and new materials, we can still observe certain side effects, including the immune reaction of the body, which may lead to the excessive inflammation. The aim of this paper was to determine how the production of prostaglandin E2 (PGE2) and thromboxane B2 (TXB2) in THP-1 monocytes\/macrophages is influenced by the titanium 3D plates and dedicated screws. The experiments were conducted on THP-1 monocytic cell line and macrophages derived from a THP-1cells. The concentrations of PGE2 and TXB2 released were measured by using immunoassay kit. Verification of plate-induced activation of THP-1 monocytes and macrophages and initiation of inflammatory reaction was conducted by flow cytometry. Despite some differences in the content of the implant devices our results showed that these plates did not statistically significantly increase the production of these prostanoids. Osteosynthesis of condylar fractures using 3D titanium mini-plates seems to be a good alternative to traditional plates due to their lack of stimulating the cyclooxygenase-dependent production of prostanoids; limiting the development of inflammatory reactions.<\/jats:p>","DOI":"10.3390\/ijms18122638","type":"journal-article","created":{"date-parts":[[2017,12,8]],"date-time":"2017-12-08T11:37:40Z","timestamp":1512733060000},"page":"2638","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["In Vitro Effect of 3D Plates Used for Surgical Treatment of Condylar Fractures on Prostaglandin E2 (PGE2) and Thromboxane B2 (TXB2) Concentration in THP-1 Macrophages"],"prefix":"10.3390","volume":"18","author":[{"given":"Maciej","family":"Sikora","sequence":"first","affiliation":[{"name":"Department of Maxillofacial Surgery, Hospital of the Ministry of Interior, Kielce, Wojska Polskiego 51, 25-375 Kielce, Poland"},{"name":"Department of Biochemistry and Medical Chemistry, Pomeranian Medical University, Powst. Wlkp. 72; 70-111 Szczecin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8730-8299","authenticated-orcid":false,"given":"Marta","family":"Goschorska","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Medical Chemistry, Pomeranian Medical University, Powst. Wlkp. 72; 70-111 Szczecin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9187-8895","authenticated-orcid":false,"given":"Irena","family":"Baranowska-Bosiacka","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Medical Chemistry, Pomeranian Medical University, Powst. Wlkp. 72; 70-111 Szczecin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4497-4395","authenticated-orcid":false,"given":"Dariusz","family":"Chlubek","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Medical Chemistry, Pomeranian Medical University, Powst. Wlkp. 72; 70-111 Szczecin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2017,12,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"630","DOI":"10.1097\/TA.0000000000000369","article-title":"Pattern of maxillofacial fractures: A 5-year analysis of 8818 patients","volume":"77","author":"Motamedi","year":"2014","journal-title":"J. Trauma Acute Care Surg."},{"key":"ref_2","first-page":"749","article-title":"Assessment of strength of titanium miniplates used for mandibular angle fractures osteosynthesis","volume":"63","author":"Gutowski","year":"2010","journal-title":"Czas. Stomatol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"306","DOI":"10.1016\/j.jcms.2006.03.003","article-title":"Open versus closed treatment of fractures of the mandibular condylar process\u2014A prospective randomized multi-centre study","volume":"34","author":"Eckelt","year":"2006","journal-title":"J. Cranio-Maxillofac. Surg."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1448","DOI":"10.1097\/SCS.0b013e31821d1814","article-title":"Complications of condylar fracture osteosynthesis","volume":"22","author":"Derfoufi","year":"2011","journal-title":"J. Craniofac. Surg."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/S0278-2391(00)80010-5","article-title":"Open treatment of condylar process fractures: Assessment of adequacy of repositioning and maintenance of stability","volume":"58","author":"Ellis","year":"2000","journal-title":"J. Oral Maxillofac. Surg."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"770","DOI":"10.1016\/j.jcms.2016.04.008","article-title":"Use of the Delta plate for surgical treatment of patients with condylar fractures","volume":"44","author":"Sikora","year":"2016","journal-title":"J. Cranio-Maxillofac. Surg."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1016\/j.jcms.2005.09.005","article-title":"Experimental evaluation of three osteosynthesis devices used for stabilizing condylar fractures of the mandible","volume":"34","author":"Meyer","year":"2006","journal-title":"J. Cranio-Maxillofac Surg."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"730","DOI":"10.1016\/j.jcms.2013.11.003","article-title":"\u201cA\u201d shape plate for open rigid internal fixation of mandible condyle neck fracture","volume":"42","author":"Kozakiewicz","year":"2014","journal-title":"J. Cranio-Maxillofac. Surg."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1054\/bjom.2001.0734","article-title":"The role of open reduction and internal fixation in unilateral fractures of the mandibular condyle: A prospective study","volume":"40","author":"Hyde","year":"2002","journal-title":"Br. J. Oral Maxillofac. Surg."},{"key":"ref_10","first-page":"45","article-title":"Plate osteosynthesis of condylar fractures: A retrospective study of 45 patients","volume":"34","author":"Rallis","year":"2003","journal-title":"Quintessence Int."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"964","DOI":"10.1016\/j.joms.2006.05.068","article-title":"A new 3-dimensional plate for transoral endoscopic-assisted osteosynthesis of condylar neck fractures","volume":"65","author":"Lauer","year":"2007","journal-title":"J. Oral Maxillofac. Surg."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1002\/jbm.a.30075","article-title":"Effect of titanium surface topography on macrophage activation and secretion of proinflammatory cytokines and chemokines","volume":"70","author":"Refai","year":"2004","journal-title":"J. Biomed. Mater. Res. A"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"953","DOI":"10.1189\/jlb.5VMR0415-166R","article-title":"Macrophage responses to implants: Prospects for personalized medicine","volume":"98","author":"Kzyshkowska","year":"2015","journal-title":"J. Leukoc. Biol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1016\/j.cyto.2011.06.020","article-title":"An in vitro evaluation of inflammation response of titanium functionalized with heparyn\/fibronectin complex","volume":"56","author":"Li","year":"2011","journal-title":"Cytokine"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"2331","DOI":"10.1016\/j.biomaterials.2005.11.044","article-title":"The significance of infection related to orthopedic devices and issues of antibiotics resistance","volume":"27","author":"Campoccia","year":"2006","journal-title":"Biomaterials"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"484","DOI":"10.1111\/j.1743-6109.2006.00138.x","article-title":"Characterization of an in vitro model for evaluating the interface between skin and percutaneous biomaterials","volume":"14","author":"Fukano","year":"2006","journal-title":"Wound Repair Regen."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"792","DOI":"10.1016\/j.biomaterials.2012.02.034","article-title":"Macrophage polarization: An opportunity for improved outcomes in biomaterials and regenerative medicine","volume":"33","author":"Brown","year":"2012","journal-title":"Biomaterials"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1902\/jop.2000.71.2.287","article-title":"Tissue response to titanium implants in the rat maxilla: Ultrastructural and histochemical observations of the bone-titanium interface","volume":"71","author":"Futami","year":"2000","journal-title":"J. Periodontol."},{"key":"ref_19","unstructured":"Auger, M.J., and Ross, J.A. (1992). The biology of the macrophage. The Macrophage, IRL Press."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"719","DOI":"10.1016\/S0923-2494(99)80045-5","article-title":"Human monocytes and cytokines","volume":"149","year":"1998","journal-title":"Res. Immunol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"977","DOI":"10.1002\/bies.950171111","article-title":"The macrophage","volume":"17","author":"Gordon","year":"1995","journal-title":"Bioessays"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1016\/0005-2760(91)90032-D","article-title":"Ptostaglandin endoperoxide synthase: Regulation of enzyme expression","volume":"1083","author":"DeWitt","year":"1991","journal-title":"Biochim. Biophys. Acta"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1007\/s12011-014-0176-4","article-title":"Cyclooxygenase-1 as the main source of proinlammatory factors after sodium orthovanadate treatment","volume":"163","author":"Korbecki","year":"2015","journal-title":"Biol. Trace Elem. Res."},{"key":"ref_24","first-page":"165","article-title":"Distinct functions of COX-1 and COX-2 Prostaglandins other","volume":"68\u201369","author":"Morita","year":"2002","journal-title":"Lipid Mediat."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"6454","DOI":"10.1074\/jbc.M111.327874","article-title":"Induction of COX-2 enzyme and down-regualtion of COX-1 expression by lipopolysaccharide (LPS) control prostaglandin E2 production in astrocytes","volume":"287","author":"Gorina","year":"2012","journal-title":"JBC"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1016\/j.jpain.2006.10.004","article-title":"Expression of COX-1and COX-2 in a clinical model of acute inflammation","volume":"8","author":"Khan","year":"2007","journal-title":"J. Pain"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"960","DOI":"10.3892\/ijmm.2012.1052","article-title":"Transcriptional and translational regulation of COX-2 in a clinical model of acute inflammation","volume":"30","author":"Park","year":"2012","journal-title":"Int. J. Mol. Med."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1016\/0142-9612(89)90019-7","article-title":"Titanium-hydrogen peroxide interaction: Model studies of the influence of the inflammatory response on titanium implants","volume":"10","author":"Tengvall","year":"1989","journal-title":"Biomaterials"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"888","DOI":"10.1302\/0301-620X.80B5.0800888","article-title":"Anti-infalammatory properties of titanium in the joint environmental","volume":"80-B","author":"Overgaard","year":"1990","journal-title":"J. Bone Jt. Surg."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"9155","DOI":"10.4238\/2015.August.7.25","article-title":"Macrophage proinflammatory response to the titanium alloy equipment in dental implantation","volume":"14","author":"Chen","year":"2015","journal-title":"Gent. Mol. Res."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1097\/BRS.0000000000000778","article-title":"Implant materials Generate Different Peri-implant inflammatory factors","volume":"40","author":"Hyzy","year":"2015","journal-title":"Spine"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1189\/jlb.0904484","article-title":"New insights into the molecular mechanism of interleukin-10-mediated immunosuppression","volume":"77","author":"Grutz","year":"2005","journal-title":"J. Leukoc. Biol."},{"key":"ref_33","first-page":"115","article-title":"Titanium-alloy particles induced cyclooxygenase-2 in human macrophage-like cells in vitro","volume":"48","author":"Nikura","year":"2002","journal-title":"Kobe J. Med. Sci."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"531","DOI":"10.1002\/jbm.a.20098","article-title":"Effect of cyclic mechanical stretch and particles on prostaglandin E2 production by human macrophages in vitro","volume":"68","author":"Fujishiro","year":"2004","journal-title":"J. Biomed. Mater. Res. A"},{"key":"ref_35","unstructured":"Surowska, B. (2009). Metal Biomaterials and Metal-Ceramic Compositions in Stomatological Application, University Publishing. [1st ed.]."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1007\/BF02041244","article-title":"The human leukemia cell line, THP1: A multifacetted model for study of monocyte-macrophage differentiation","volume":"47","author":"Auwerx","year":"1991","journal-title":"Experientia"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1016\/j.atherosclerosis.2011.09.003","article-title":"The use of THP-1 cells as a model for mimicking the function and regulation of monocytes and macrophages in the vasculature","volume":"221","author":"Qin","year":"2012","journal-title":"Atherosclerosis"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1007\/s12011-015-0234-6","article-title":"The Effect of Cadmium on COX-1 and COX-2 Gene, Protein Expression, and Enzymatic Activity in THP-1 Macrophages","volume":"165","author":"Olszowski","year":"2015","journal-title":"Biol. Trace Elem. Res."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1661","DOI":"10.1016\/j.tiv.2015.06.024","article-title":"Fluoride as a factor initiating and potentiating inflammation in THP1 differentiated monocytes\/macrophages","volume":"29","author":"Gutowska","year":"2015","journal-title":"Toxicol. In Vitro"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1197","DOI":"10.1161\/01.RES.0000020017.84398.61","article-title":"Platelet phagocytosis and processing of beta-amyloid precursor protein as a mechanism of macrophage activation in atherosclerosis","volume":"90","author":"Cooper","year":"2002","journal-title":"Circ. Res."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1172\/JCI200113416","article-title":"Mixed messages: Modulation of inflammation and immune responses by prostaglandins and thromboxanes","volume":"108","author":"Tilley","year":"2001","journal-title":"J. Clin. Investig."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/18\/12\/2638\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T18:53:13Z","timestamp":1760208793000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/18\/12\/2638"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,12,8]]},"references-count":41,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2017,12]]}},"alternative-id":["ijms18122638"],"URL":"https:\/\/doi.org\/10.3390\/ijms18122638","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,12,8]]}}}