{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T12:17:57Z","timestamp":1774959477020,"version":"3.50.1"},"reference-count":30,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2017,12,25]],"date-time":"2017-12-25T00:00:00Z","timestamp":1514160000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>MicroRNAs (miRNAs) are small non-coding RNAs that act as regulators of gene expression at the post-transcriptional level. They play a key role in several biological processes. Their abnormal expression may lead to malignant cell transformation. This study aimed to evaluate the expression profile of 84 miRNAs involved in tumorigenesis in immortalized cells of myometrium (MM), uterine leiomyoma (ULM), and uterine leiomyosarcoma (ULMS). Specific cell lines were cultured and qRT-PCR was performed. Thirteen miRNAs presented different expression profiles in ULM and the same thirteen in ULMS compared to MM. Eight miRNAs were overexpressed, and five were underexpressed in ULM. In ULMS cells, five miRNAs exhibited an overexpression and eight were down-regulated. Six miRNAs (miR-1-3p, miR-130b-3p, miR-140-5p, miR-202-3p, miR-205-5p, and miR-7-5p) presented a similar expression pattern in cell lines compared to patient samples. Of these, only three miRNAs showed significant expression in ULM (miR-1-3p, miR-140-5p, and miR-7-5p) and ULMS (miR-1-3p, miR-202-3p, and miR-7-5p). Our preliminary approach identified 24 oncomirs with an altered expression profile in ULM and ULMS cells. We identified four differentially expressed miRNAs with the same profile when compared with patients\u2019 samples, which strongly interacted with relevant genes, including apoptosis regulator (BCL2), epidermal growth factor receptor (EGFR), vascular endothelial growth factor A (VEGFA), insulin like growth factor 1 receptor (IGF1R),serine\/threonine kinase (RAF1), receptor tyrosine kinase (MET), and bHLH transcription factor (MYCN). This led to alterations in their mRNA-target.<\/jats:p>","DOI":"10.3390\/ijms19010052","type":"journal-article","created":{"date-parts":[[2017,12,26]],"date-time":"2017-12-26T03:06:38Z","timestamp":1514257598000},"page":"52","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Oncomirs Expression Profiling in Uterine Leiomyosarcoma Cells"],"prefix":"10.3390","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1641-5609","authenticated-orcid":false,"given":"Bruna","family":"De Almeida","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Ginecologia Estrutural e Molecular (LIM 58), Disciplina de Ginecologia, Departamento de Obstetricia e Ginecologia, Hospital das Clinicas da Faculdade de Medicina da Universidade de Sao Paulo, HCFMUSP, SP, BR Av. Dr Arnaldo 455, sala 4121, 05403-010 Cerqueira Cesar, S\u00e3o Paulo, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Natalia","family":"Garcia","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Ginecologia Estrutural e Molecular (LIM 58), Disciplina de Ginecologia, Departamento de Obstetricia e Ginecologia, Hospital das Clinicas da Faculdade de Medicina da Universidade de Sao Paulo, HCFMUSP, SP, BR Av. Dr Arnaldo 455, sala 4121, 05403-010 Cerqueira Cesar, S\u00e3o Paulo, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giovana","family":"Maffazioli","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Ginecologia Estrutural e Molecular (LIM 58), Disciplina de Ginecologia, Departamento de Obstetricia e Ginecologia, Hospital das Clinicas da Faculdade de Medicina da Universidade de Sao Paulo, HCFMUSP, SP, BR Av. Dr Arnaldo 455, sala 4121, 05403-010 Cerqueira Cesar, S\u00e3o Paulo, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura","family":"Gonzalez dos Anjos","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Ginecologia Estrutural e Molecular (LIM 58), Disciplina de Ginecologia, Departamento de Obstetricia e Ginecologia, Hospital das Clinicas da Faculdade de Medicina da Universidade de Sao Paulo, HCFMUSP, SP, BR Av. Dr Arnaldo 455, sala 4121, 05403-010 Cerqueira Cesar, S\u00e3o Paulo, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edmund","family":"Chada Baracat","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Ginecologia Estrutural e Molecular (LIM 58), Disciplina de Ginecologia, Departamento de Obstetricia e Ginecologia, Hospital das Clinicas da Faculdade de Medicina da Universidade de Sao Paulo, HCFMUSP, SP, BR Av. Dr Arnaldo 455, sala 4121, 05403-010 Cerqueira Cesar, S\u00e3o Paulo, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katia","family":"Candido Carvalho","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Ginecologia Estrutural e Molecular (LIM 58), Disciplina de Ginecologia, Departamento de Obstetricia e Ginecologia, Hospital das Clinicas da Faculdade de Medicina da Universidade de Sao Paulo, HCFMUSP, SP, BR Av. Dr Arnaldo 455, sala 4121, 05403-010 Cerqueira Cesar, S\u00e3o Paulo, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2017,12,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1016\/j.ygeno.2012.03.003","article-title":"Characterization of the uterine leiomyoma microRNAome by deep sequencing","volume":"99","author":"Georgieva","year":"2012","journal-title":"Genomics"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1016\/j.csbj.2016.10.005","article-title":"Functional roles for exosomal microRNAs in the tumour microenvironment","volume":"15","author":"Bell","year":"2017","journal-title":"Comput. Struct. Biotechnol. J."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"2153","DOI":"10.1007\/s13277-013-0748-5","article-title":"microRNAs in uterine sarcomas and mixed epithelial-mesenchymal uterine tumors: A preliminary report","volume":"34","author":"Kowalewska","year":"2013","journal-title":"Tumour Biol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/978-94-007-5590-1_1","article-title":"MicroRNA cancer regulation","volume":"774","author":"Farazi","year":"2013","journal-title":"Adv. Exp. Med. Biol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"908","DOI":"10.2353\/ajpath.2010.091150","article-title":"A differentiation-based microRNA signature identifies leiomyosarcoma as a mesenchymal stem cell-related malignancy","volume":"177","author":"Danielson","year":"2010","journal-title":"Am. J. Pathol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1303","DOI":"10.1038\/modpathol.2009.96","article-title":"Molecular and immunohistochemical evidence for the origin of uterine leiomyosarcomas from associated leiomyoma and symplastic leiomyoma-like areas","volume":"22","author":"Mittal","year":"2009","journal-title":"Mod. Pathol."},{"key":"ref_7","first-page":"291","article-title":"Clinical and molecular features of uterine sarcomas","volume":"1","author":"Maciel","year":"2014","journal-title":"Med. Express"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1111\/j.1365-2559.2009.03231.x","article-title":"Uterine sarcomas in Norway. A histopathological and prognostic survey of a total population from 1970 to 2000 including 419 patients","volume":"54","author":"Abeler","year":"2009","journal-title":"Histopathology"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1571","DOI":"10.1016\/j.humpath.2009.03.018","article-title":"Comparative clinicopathologic and immunohistochemical analysis of uterine sarcomas diagnosed using the World Health Organization classification system","volume":"40","author":"Spagnoli","year":"2009","journal-title":"Hum. Pathol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"563","DOI":"10.1177\/1933719114553450","article-title":"The regulatory function of miR-200c on inflammatory and cell-cycle associated genes in SK-LMS-1, a leiomyosarcoma cell line","volume":"22","author":"Chuang","year":"2015","journal-title":"Reprod. Sci."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Kobayashi, H. (2013). The biology of uterine sarcomas: A review and update. Mol. Clin. Oncol., 599\u2013609.","DOI":"10.3892\/mco.2013.124"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"693","DOI":"10.1002\/gcc.22179","article-title":"Differentiating soft tissue leiomyosarcoma and undifferentiated pleomorphic sarcoma: A miRNA analysis","volume":"53","author":"Guled","year":"2014","journal-title":"Genes Chromosomes Cancer"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/j.humpath.2015.08.026","article-title":"May Sonic Hedgehog proteins be markers for malignancy in uterine smooth muscle tumors?","volume":"50","author":"Garcia","year":"2016","journal-title":"Hum. Pathol."},{"key":"ref_14","unstructured":"Stewart, E.A. (2017, September 06). Differentiating Uterine Leiomyomas (Fibroids) from Uterine Sarcomas\u2014UpToDate. Available online: https:\/\/www.uptodate.com\/contents\/differentiating-uterine-leiomyomas-fibroids-from-uterine-sarcomas."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1155\/2017\/2827435","article-title":"miR-202 suppresses cell proliferation by targeting FOXR2 in Endometrial adenocarcinoma","volume":"2017","author":"Deng","year":"2017","journal-title":"Dis. Markers"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1146","DOI":"10.1158\/1078-0432.CCR-13-1023","article-title":"MicroRNA-202-3p inhibits cell proliferation by targeting ADP-ribosylation factor-like 5A in human colorectal carcinoma","volume":"20","author":"Wang","year":"2014","journal-title":"Clin. Cancer Res."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"115","DOI":"10.4161\/15384047.2014.987070","article-title":"Down regulation of miR-202 modulates Mxd1 and Sin3A repressor complexes to induce apoptosis of pancreatic cancer cells","volume":"16","author":"Farhana","year":"2015","journal-title":"Cancer Biol. Ther."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"41","DOI":"10.3892\/or.2016.5274","article-title":"Potential role of BCL2 in the recurrence of uterine smooth muscle tumors of uncertain malignant potential","volume":"37","author":"Conconi","year":"2017","journal-title":"Oncol. Rep."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"103","DOI":"10.3892\/ol.2017.6165","article-title":"Effect of 2-methoxyestradiol on SK-LMS-1 uterine leiomyosarcoma cells","volume":"14","author":"Lee","year":"2017","journal-title":"Oncol. Lett."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1016\/j.ygyno.2010.11.031","article-title":"The expression of hepatocyte growth factor (HGF) and c-Met in uterine serous carcinoma","volume":"121","author":"Bishop","year":"2011","journal-title":"Gynecol. Oncol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1415","DOI":"10.1515\/cclm-2014-0798","article-title":"Expression of vascular endothelial factor-A, gelatinases (MMP-2, MMP-9) and TIMP-1 in uterine leiomyomas","volume":"53","author":"Korompelis","year":"2015","journal-title":"Clin. Chem. Lab. Med."},{"key":"ref_22","first-page":"637","article-title":"The role of insulin-like growth factor-1 signaling pathways in uterine leiomyoma","volume":"29","author":"Gkioka","year":"2015","journal-title":"In Vivo"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"772","DOI":"10.1093\/humupd\/dmr031","article-title":"Growth factors and myometrium: Biological effects in uterine fibroid and possible clinical implications","volume":"17","author":"Ciarmela","year":"2011","journal-title":"Hum. Reprod. Update"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"2014","DOI":"10.1038\/onc.2016.384","article-title":"The lack of Raf-1 kinase feedback regulation enhances antiapoptosis in cancer cells","volume":"36","author":"Ma","year":"2017","journal-title":"Oncogene"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"311","DOI":"10.3322\/caac.21244","article-title":"MicroRNAome genome: A treasure for cancer diagnosis and therapy","volume":"64","author":"Monroig","year":"2014","journal-title":"CA Cancer J. Clin."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1172","DOI":"10.1016\/j.cell.2012.02.005","article-title":"MicroRNAs in stress signaling and human disease","volume":"148","author":"Mendell","year":"2013","journal-title":"Cell"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"670","DOI":"10.1093\/humupd\/dmu017","article-title":"MicroRNAs in the development and pathobiology of uterine leiomyomata: Does evidence support future strategies for clinical intervention?","volume":"20","author":"Karmon","year":"2014","journal-title":"Hum. Reprod. Update"},{"key":"ref_28","unstructured":"(2017, September 18). Genorm. Available online: https:\/\/genorm.cmgg.be\/."},{"key":"ref_29","unstructured":"(2017, September 18). miScript miRNA PCR Array Data Analysis. Available online: http:\/\/pcrdataanalysis.sabiosciences.com\/mirna\/arrayanalysis.php."},{"key":"ref_30","unstructured":"(2017, August 13). miRTarBase. Available online: http:\/\/mirtarbase.mbc.nctu.edu.tw\/php\/index.php."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/19\/1\/52\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T18:55:24Z","timestamp":1760208924000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/19\/1\/52"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,12,25]]},"references-count":30,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2018,1]]}},"alternative-id":["ijms19010052"],"URL":"https:\/\/doi.org\/10.3390\/ijms19010052","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,12,25]]}}}