{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,11]],"date-time":"2026-05-11T22:23:21Z","timestamp":1778538201645,"version":"3.51.4"},"reference-count":43,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2018,2,28]],"date-time":"2018-02-28T00:00:00Z","timestamp":1519776000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Background: The immunohistochemical expression of aquaporin-1 (AQP1) in asbestos-related malignant pleural mesothelioma (MPM) is emerging as a useful prognostic indicator of improved survival. A significantly increased incidence of MPM in a small town in southern Italy was ascribed to exposure to fluoro-edenite (FE), a naturally occurring asbestos fiber. We investigated the immunohistochemical expression of AQP1 in patients affected by FE-related MPM; taking into consideration its suggested independent prognostic role, its possible correlation with clinicopathological parameters and patient outcome was also evaluated. Methods: Ten patients were selected for this study, as neoplastic tissue blocks, clinical and follow-up data were available. The immunohistochemical overexpression of AQP1 was defined as \u226550% of tumor cells showing membranous staining. Results: Six cases showed AQP1 expression in \u226550% of tumor cells; in this group, a significant association of AQP1 overexpression with an increased median overall survival (OS) of 26.3 months was observed. By contrast, four patients exhibited an AQP1 score of &lt;50% of stained cells, with a shorter median OS of 8.9 months. Conclusions: The present study represents further confirmation of the hypothesized prognostic role of AQP1, which seems a reliable prognostic indicator.<\/jats:p>","DOI":"10.3390\/ijms19030685","type":"journal-article","created":{"date-parts":[[2018,2,28]],"date-time":"2018-02-28T12:54:12Z","timestamp":1519822452000},"page":"685","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Immunohistochemical Expression of Aquaporin-1 in Fluoro-Edenite-Induced Malignant Mesothelioma: A Preliminary Report"],"prefix":"10.3390","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4808-2052","authenticated-orcid":false,"given":"Giuseppe","family":"Angelico","sequence":"first","affiliation":[{"name":"Anatomic Pathology, Department of Human Pathology in Adult and Developmental Age \u201cGaetano Barresi\u201d, University of Messina, 98122 Messina, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8591-8010","authenticated-orcid":false,"given":"Rosario","family":"Caltabiano","sequence":"additional","affiliation":[{"name":"Anatomic Pathology, Department of Medical and Surgical Sciences and Advanced Technologies, G.F. Ingrassia, University of Catania, 95124 Catania, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6301-9743","authenticated-orcid":false,"given":"Carla","family":"Loreto","sequence":"additional","affiliation":[{"name":"Anatomy and Histology, Department of Biomedical Sciences and Biotechnologies, University of Catania, 95124 Catania, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2878-3572","authenticated-orcid":false,"given":"Antonio","family":"Ieni","sequence":"additional","affiliation":[{"name":"Anatomic Pathology, Department of Human Pathology in Adult and Developmental Age \u201cGaetano Barresi\u201d, University of Messina, 98122 Messina, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1561-4246","authenticated-orcid":false,"given":"Giovanni","family":"Tuccari","sequence":"additional","affiliation":[{"name":"Anatomic Pathology, Department of Human Pathology in Adult and Developmental Age \u201cGaetano Barresi\u201d, University of Messina, 98122 Messina, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0739-8798","authenticated-orcid":false,"given":"Caterina","family":"Ledda","sequence":"additional","affiliation":[{"name":"Occupational Medicine, Department of Clinical and Experimental Medicine, University of Catania, 95124 Catania, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Venerando","family":"Rapisarda","sequence":"additional","affiliation":[{"name":"Occupational Medicine, Department of Clinical and Experimental Medicine, University of Catania, 95124 Catania, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2018,2,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"v31","DOI":"10.1093\/annonc\/mdv199","article-title":"ESMO Guidelines Committee. Malignant pleural mesothelioma: ESMO Clinical Practice Guidelines for diagnosis, treatment and follow-up","volume":"26","author":"Baas","year":"2015","journal-title":"Ann. Oncol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1186\/s12995-016-0127-4","article-title":"Malignant mesothelioma incidence by nation-wide cancer registry: A population-based study","volume":"11","author":"Tomasson","year":"2016","journal-title":"J. Occup. Med. Toxicol."},{"key":"ref_3","first-page":"4563217741145","article-title":"Biomarkers in mesothelioma","volume":"1","author":"Arnold","year":"2017","journal-title":"Ann. Clin. Biochem."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"716","DOI":"10.2471\/BLT.11.086678","article-title":"Global mesothelioma deaths reported to the World Health Organization between 1994 and 2008","volume":"89","author":"Delgermaa","year":"2011","journal-title":"Bull. World Health Organ."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"2952","DOI":"10.1002\/cncr.26497","article-title":"Aquaporin 1 is an independent prognostic factor in pleural malignant mesothelioma","volume":"118","author":"Kao","year":"2012","journal-title":"Cancer"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Driml, J., Pulford, E., Moffat, D., Karapetis, C., Kao, S., Griggs, K., Henderson, D.W., and Klebe, S. (2016). Usefulness of Aquaporin 1 as a Prognostic Marker in a Prospective Cohort of Malignant Mesotheliomas. Int. J. Mol. Sci., 17.","DOI":"10.3390\/ijms17071041"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1492","DOI":"10.1016\/j.bbagen.2013.09.033","article-title":"Human aquaporins: Regulators of transcellular water flow","volume":"1840","author":"Day","year":"2014","journal-title":"Biochim. Biophys. Acta"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Pelagalli, A., Squillacioti, C., Mirabella, N., and Meli, R. (2016). Aquaporins in Health and Disease: An Overview Focusing on the Gut of Different Species. Int. J. Mol. Sci., 17.","DOI":"10.3390\/ijms17081213"},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Pulford, E., McEvoy, J., Hocking, A., Prabhakaran, S., Griggs, K., and Klebe, S. (2017). The Effect of Aquaporin 1-Inhibition on Vasculogenic Mimicry in Malignant Mesothelioma. Int. J. Mol. Sci., 18.","DOI":"10.3390\/ijms18112293"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"L489","DOI":"10.1152\/ajplung.00410.2015","article-title":"Influence of AQP1 on cell adhesion, migration, and tumor sphere formation in malignant pleural mesothelioma is substratum- and histological-type dependent","volume":"310","author":"Jagirdar","year":"2016","journal-title":"Am. J. Physiol. Lung Cell. Mol. Physiol."},{"key":"ref_11","first-page":"286719","article-title":"Blockade of aquaporin 1 inhibits proliferation, motility, and metastatic potential of mesothelioma in vitro but not in an in vivo model","volume":"2015","author":"Klebe","year":"2015","journal-title":"Dis. Mark."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Tomita, Y., Dorward, H., Yool, A.J., Smith, E., Townsend, A.R., Price, T.J., and Hardingham, J.E. (2017). Role of Aquaporin 1 Signalling in Cancer Development and Progression. Int. J. Mol. Sci., 18.","DOI":"10.3390\/ijms18020299"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"2046","DOI":"10.3892\/ol.2015.2978","article-title":"Detection of pleural plaques in workers exposed to inhalation of natural fluoro-edenitefibres","volume":"9","author":"Rapisarda","year":"2015","journal-title":"Oncol. Lett."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"35","DOI":"10.2217\/fon.15.271","article-title":"FBLN-3 as a biomarker of pleural plaques in workers occupationally exposed to carcinogenic fibers: A pilot study","volume":"11","author":"Rapisarda","year":"2015","journal-title":"Future Oncol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1016\/j.envres.2016.01.043","article-title":"Sheep lymph-nodes as a biological indicator of environmental exposure to fluoro-edenite","volume":"147","author":"Ledda","year":"2016","journal-title":"Environ. Res."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/j.envres.2016.05.024","article-title":"Natural carcinogenicfiber and pleuralplaquesassessment in a general population: A cross-sectionalstudy","volume":"150","author":"Ledda","year":"2016","journal-title":"Environ. Res."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"59","DOI":"10.2217\/fon-2016-0338","article-title":"Early effects of fluoro-edenite: Correlation between IL-18 serum levels and pleural and parenchymal abnormalities","volume":"12","author":"Ledda","year":"2016","journal-title":"Future Oncol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"63","DOI":"10.2217\/fon-2016-0337","article-title":"High risk of pleural plaques and parenchymal abnormalities in women living in biancavilla (Italy)","volume":"12","author":"Ledda","year":"2016","journal-title":"Future Oncol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1016\/j.envres.2017.03.055","article-title":"Analysis of fibulin-3 afterexposure to asbestos-likefibers","volume":"156","author":"Rapisarda","year":"2017","journal-title":"Environ. Res."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"3372","DOI":"10.3892\/mmr.2017.6384","article-title":"Immunomodulatory effects in workers exposed to naturally occurring asbestos fibers","volume":"15","author":"Ledda","year":"2017","journal-title":"Mol. Med. Rep."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Ledda, C., Caltabiano, R., Loreto, C., Cin\u00e0, D., Senia, P., Musumeci, A., Ricceri, R., Pomara, C., and Rapisarda, V. (2018). Prevalence of anti-nuclear autoantibodies in subjects exposed to natural asbestiform fibers: A cross-sectional study. J. Immunotoxicol.","DOI":"10.1080\/1547691X.2017.1415398"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Rapisarda, V., Loreto, C., Castorina, S., Romano, G., Garozzo, S.F., Musumeci, A., Migliore, M., Avola, R., Cin\u00e0, D., and Pomara, C. (2018). Occupational exposure to Fluoro-edenite and prevalence of anti-nuclearautoantibodies. Future Oncol.","DOI":"10.2217\/fon-2017-0389"},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Caltabiano, R., Loreto, C., Vitale, E., Matera, S., Miozzi, E., Migliore, M., Angelico, G., Tumino, R., Ledda, C., and Rapisarda, V. (2018). Fibulin-3 immunoexpression in malignantmesothelioma due to Fluoro-edenite: A preliminary report. Future Oncol.","DOI":"10.2217\/fon-2017-0386"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"229","DOI":"10.3200\/AEOH.58.4.229-232","article-title":"Pleuralmesotheliomacases in Biancavilla are related to a new fluoro-edenite fibrousamphibole","volume":"58","author":"Comba","year":"2003","journal-title":"Arch. Environ. Health"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"392","DOI":"10.1080\/00039890009604036","article-title":"Unusually high incidence of malignant pleural mesothelioma in a town of eastern Sicily: An epidemiological and environmental study","volume":"55","author":"Paoletti","year":"2000","journal-title":"Arch. Environ. Health"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1186\/1476-069X-5-20","article-title":"Fluoro-edeniticfibres in the sputum of subjects from Biancavilla (Sicily): A pilot study","volume":"5","author":"Putzu","year":"2006","journal-title":"Environ. Health"},{"key":"ref_27","first-page":"319","article-title":"Fluoro-edenite fibres induce lungcellapoptosis: An in vivo study","volume":"23","author":"Loreto","year":"2008","journal-title":"Histol. Histopathol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1427","DOI":"10.1016\/S1470-2045(14)71109-X","article-title":"International Agency for Research on Cancer Monograph Working Group. Carcinogenicity of fluoro-edenite, silicon carbide fibres and whiskers, and carbon nanotubes","volume":"15","author":"Grosse","year":"2014","journal-title":"Lancet Oncol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1016\/j.jtho.2015.11.005","article-title":"World Health Organization CoMMittee for Tumors of the Pleura. The 2015 World Health Organization Classification of Tumors of the Pleura: Advances since the 2004 Classification","volume":"11","author":"Churg","year":"2016","journal-title":"J. Thorac. Oncol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/j.gene.2012.12.075","article-title":"Gene expression profile of aquaporin 1 and associated interactors in malignant pleural mesothelioma","volume":"517","author":"Jagirdar","year":"2013","journal-title":"Gene"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"8143","DOI":"10.18632\/oncotarget.6994","article-title":"Expression of aquaporin1, a water channel protein, in cytoplasm is negatively correlated with prognosis of breast cancer patients","volume":"7","author":"Qin","year":"2016","journal-title":"Oncotarget"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1007\/s12253-016-0145-7","article-title":"Overexpression of aquaporin-1 is a prognostic factor for biochemical recurrence in prostate adenocarcinoma","volume":"23","author":"Park","year":"2016","journal-title":"Pathol. Oncol. Res."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"251","DOI":"10.4132\/jptm.2016.03.30","article-title":"Aquaporin 1 Is an Independent Marker of Poor Prognosis in Lung Adenocarcinoma","volume":"50","author":"Yun","year":"2016","journal-title":"J. Pathol. Transl. Med."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Maugeri, R., Schiera, G., di Liegro, C.M., Fricano, A., Iacopino, D.G., and di Liegro, I. (2016). Aquaporins and Brain Tumors. Int. J. Mol. Sci., 17.","DOI":"10.3390\/ijms17071029"},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Chen, R., Shi, Y., Amiduo, R., Tuokan, T., and Suzuk, L. (2014). Expression and prognostic value of aquaporin 1, 3 in cervical carcinoma in women of Uygur ethnicity from Xinjiang, China. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0098576"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1159\/000443843","article-title":"Expression of AQP1 Was Associated with Apoptosis and Survival of Patients in Gastric Adenocarcinoma","volume":"33","author":"Sun","year":"2016","journal-title":"Dig. Surg."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1016\/j.humpath.2016.07.038","article-title":"Diagnostic utility of cyclin D1 in the diagnosis of small round blue cell tumors in children and adolescents","volume":"60","author":"Magro","year":"2017","journal-title":"Hum. Pathol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1016\/j.arcmed.2016.08.009","article-title":"Malignant pleural mesothelioma: The need to move from research to clinical practice","volume":"47","author":"Ledda","year":"2016","journal-title":"Arch. Med. Res."},{"key":"ref_39","first-page":"2775","article-title":"Aquaporin 3 Expression Predicts Survival in Patients with HER2-positive Early Breast Cancer","volume":"35","author":"Kang","year":"2015","journal-title":"Anticancer Res."},{"key":"ref_40","first-page":"ii14","article-title":"AQP1 expression predicts survival in patients with colon cancer","volume":"25","author":"Ohkyoung","year":"2014","journal-title":"Ann. Oncol."},{"key":"ref_41","first-page":"1741","article-title":"Prognostic significance of aquaporins in human biliary tract carcinoma","volume":"27","author":"Sekine","year":"2012","journal-title":"Oncol. Rep."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"e1261241","DOI":"10.1080\/2162402X.2016.1261241","article-title":"Prognostic and predictive aspects of the tumor immune microenvironment and immune checkpoints in malignant pleural mesothelioma","volume":"6","author":"Marcq","year":"2016","journal-title":"Oncoimmunology"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"73296","DOI":"10.18632\/oncotarget.20265","article-title":"Tumor-educated mesenchymal stem cells promote pro-metastatic phenotype","volume":"8","author":"Hill","year":"2017","journal-title":"Oncotarget"}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/19\/3\/685\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T14:56:53Z","timestamp":1760194613000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/19\/3\/685"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,2,28]]},"references-count":43,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2018,3]]}},"alternative-id":["ijms19030685"],"URL":"https:\/\/doi.org\/10.3390\/ijms19030685","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,2,28]]}}}