{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,10]],"date-time":"2026-03-10T13:08:53Z","timestamp":1773148133953,"version":"3.50.1"},"reference-count":56,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2018,5,9]],"date-time":"2018-05-09T00:00:00Z","timestamp":1525824000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Magnesium plays a pivotal role in energy metabolism and in the control of cell growth. While magnesium deprivation clearly shapes the behavior of normal and neoplastic cells, little is known on the role of this element in cell differentiation. Here we show that magnesium deficiency increases the transcription of multipotency markers and tissue-specific transcription factors in human adipose-derived mesenchymal stem cells exposed to a mixture of natural molecules, i.e., hyaluronic, butyric and retinoid acids, which tunes differentiation. We also demonstrate that magnesium deficiency accelerates the osteogenic differentiation of human bone marrow-derived mesenchymal stem cells. We argue that magnesium deprivation generates a stressful condition that modulates stem cell plasticity and differentiation potential. These studies indicate that it is possible to remodel transcription in mesenchymal stem cells by lowering extracellular magnesium without the need for genetic manipulation, thus offering new hints for regenerative medicine applications.<\/jats:p>","DOI":"10.3390\/ijms19051410","type":"journal-article","created":{"date-parts":[[2018,5,10]],"date-time":"2018-05-10T03:48:27Z","timestamp":1525924107000},"page":"1410","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Magnesium Deprivation Potentiates Human Mesenchymal Stem Cell Transcriptional Remodeling"],"prefix":"10.3390","volume":"19","author":[{"given":"Azzurra","family":"Sargenti","sequence":"first","affiliation":[{"name":"Department of Pharmacy and Biotechnology, University of Bologna, 40127 Bologna, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8785-9891","authenticated-orcid":false,"given":"Sara","family":"Castiglioni","sequence":"additional","affiliation":[{"name":"Department of Biomedical and Clinical Sciences \u2018L. Sacco\u2019, University of Milan, 20157 Milan, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elena","family":"Olivi","sequence":"additional","affiliation":[{"name":"GUNA\u2014ATTRE (Advanced Therapies and Tissue Regeneration), Innovation Accelerator at CNR, Via Gobetti 101, 40129 Bologna, Italy"},{"name":"National Institute of Biostructures and Biosystems (NIBB), 00136 Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francesca","family":"Bianchi","sequence":"additional","affiliation":[{"name":"National Institute of Biostructures and Biosystems (NIBB), 00136 Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alessandra","family":"Cazzaniga","sequence":"additional","affiliation":[{"name":"Department of Biomedical and Clinical Sciences \u2018L. Sacco\u2019, University of Milan, 20157 Milan, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giovanna","family":"Farruggia","sequence":"additional","affiliation":[{"name":"Department of Pharmacy and Biotechnology, University of Bologna, 40127 Bologna, Italy"},{"name":"National Institute of Biostructures and Biosystems (NIBB), 00136 Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3874-6815","authenticated-orcid":false,"given":"Concettina","family":"Cappadone","sequence":"additional","affiliation":[{"name":"Department of Pharmacy and Biotechnology, University of Bologna, 40127 Bologna, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5712-4272","authenticated-orcid":false,"given":"Lucia","family":"Merolle","sequence":"additional","affiliation":[{"name":"Transfusion Medicine Unit, Azienda Usl di Reggio Emilia-IRCCS, 42123 Reggio Emilia, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emil","family":"Malucelli","sequence":"additional","affiliation":[{"name":"Department of Pharmacy and Biotechnology, University of Bologna, 40127 Bologna, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9333-0321","authenticated-orcid":false,"given":"Carlo","family":"Ventura","sequence":"additional","affiliation":[{"name":"GUNA\u2014ATTRE (Advanced Therapies and Tissue Regeneration), Innovation Accelerator at CNR, Via Gobetti 101, 40129 Bologna, Italy"},{"name":"National Institute of Biostructures and Biosystems (NIBB), 00136 Rome, Italy"},{"name":"National Laboratory of Molecular Biology and Stem Cell Engineering\u2014Eldor Lab, Innovation Accelerator at CNR, Via Gobetti 101, 40129 Bologna, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeanette A. 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