{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:46:47Z","timestamp":1787028407540,"version":"build-2736575974"},"reference-count":83,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2018,6,26]],"date-time":"2018-06-26T00:00:00Z","timestamp":1529971200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Breast Cancer (BC) encompasses numerous entities with different biological and behavioral characteristics, favored by tumor molecular complexity. Azadirachta indica (neem) presents phenolic compounds, indicating its potential as an antineoplastic compound. The present study aimed to evaluate the cellular response of MCF10, MCF7, and MDA-MB-231 breast cell lines to ethanolic extracts of neem leaves (EENL) obtained by dichloromethane (DCM) and ethyl acetate (EA) solvent. Extracts\u2019 antiproliferative activities were evaluated against MCF 10A, MCF7, and MDA-MB-231 for 24 and 48 h using MTT assay. ESR1, ESR2, AR, AR-V1, AR-V4, and AR-V7 transcripts were quantified through qPCR for 0.03125 \u03bcg\/mL of DCM and 1.0 \u03bcg\/mL for EA for 48 h. The EENL was tested on Drosophila melanogaster as a sole treatment and then also together with doxorubicin. Antiproliferative effect on tumor cell lines without affecting MCF 10A were 1.0 \u00b5g\/mL (P &lt; 0.001) for EA, and 0.03125 \u00b5g\/mL (P &lt; 0.0001) for DCM, both after 48 h. Transcriptional levels of AR-V7 increased after treatment. In vivo assays demonstrated that EENL induced fewer tumors at a higher concentration with doxorubicin (DXR). The behavior of AR-V7 in the MDA-MB-231 tumor lineage indicates new pathways involved in tumor biology and this may have therapeutic value for cancer.<\/jats:p>","DOI":"10.3390\/ijms19071879","type":"journal-article","created":{"date-parts":[[2018,6,26]],"date-time":"2018-06-26T10:40:50Z","timestamp":1530009650000},"page":"1879","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Ethanolic Extracts from Azadirachta indica Leaves Modulate Transcriptional Levels of Hormone Receptor Variant in Breast Cancer Cell Lines"],"prefix":"10.3390","volume":"19","author":[{"given":"Deisi L.","family":"Braga","sequence":"first","affiliation":[{"name":"Laboratory of Genetics and Biotechnology, Institute of Biotechnology, Federal University of Uberlandia, Uberlandia-MG 38700-128, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara T. S.","family":"Mota","sequence":"additional","affiliation":[{"name":"Laboratory of Genetics and Biotechnology, Institute of Biotechnology, Federal University of Uberlandia, Uberlandia-MG 38700-128, Brazil"},{"name":"Laboratory of Nanobiotechnology, Institute of Biotechnology, Federal University of Uberlandia, Uberlandia-MG 38400-902, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariana A. P.","family":"Z\u00f3ia","sequence":"additional","affiliation":[{"name":"Laboratory of Nanobiotechnology, Institute of Biotechnology, Federal University of Uberlandia, Uberlandia-MG 38400-902, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paula M. A. P.","family":"Lima","sequence":"additional","affiliation":[{"name":"Laboratory of Cytogenetic and Mutagenesis, University Center of Patos de Minas, Patos de Minas-MG 38700-207, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Priscila C.","family":"Orsolin","sequence":"additional","affiliation":[{"name":"Laboratory of Cytogenetic and Mutagenesis, University Center of Patos de Minas, Patos de Minas-MG 38700-207, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lara","family":"Vecchi","sequence":"additional","affiliation":[{"name":"Laboratory of Nanobiotechnology, Institute of Biotechnology, Federal University of Uberlandia, Uberlandia-MG 38400-902, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00falio C.","family":"Nepomuceno","sequence":"additional","affiliation":[{"name":"Laboratory of Cytogenetic and Mutagenesis, University Center of Patos de Minas, Patos de Minas-MG 38700-207, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristina R.","family":"F\u00fcrstenau","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Cell Culture, Institute of Biotechnology, Federal University of Uberlandia, Uberlandia-MG 38700-128, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9169-5028","authenticated-orcid":false,"given":"Yara C. 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