{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T13:19:19Z","timestamp":1789823959737,"version":"4.0.1"},"reference-count":25,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2018,8,6]],"date-time":"2018-08-06T00:00:00Z","timestamp":1533513600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81273375"],"award-info":[{"award-number":["81273375"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Integrin \u03b1IIb\u03b23 plays a pivotal role in platelet aggregation. Three \u03b1IIb\u03b23 antagonists have been approved by the Food and Drug Administration (FDA) for the treatment of cardiovascular diseases. Unfortunately, all of these three drugs can cause the side effect of severe bleeding. Therefore, developing a new \u03b1IIb\u03b23 antagonist with low bleeding was needed. In the present study, we screened compounds by using a fibrinogen\/integrin \u03b1IIb\u03b23 enzyme-linked immunosorbent assay (ELISA), and a novel \u03b1IIb\u03b23 antagonist ANTP266 was attained. The antithrombotic effects of ANTP266 were estimated by using two animal models, the bleeding risk was estimated by using a mice tail cutting assay, and the plasma half-life time was tested by LC-MS\/MS. The results showed that ANTP266 potently decreased thrombosis formation, while not prolonging bleeding time at its effective dosage. The bleeding of ANTP266 reduced rapidly as time went on from 5 to 60 min, but tirofiban produced high bleeding continuously. The plasma half-life of ANTP266 in rats was 10.8 min. Taken together, ANTP266 is an effective antithrombotic agent with a low bleeding risk. The shorter bleeding time benefits from its short plasma half-life. ANTP266 could be a candidate for developing the \u03b1IIb\u03b23 antagonist of rapid elimination for a patient undergoing percutaneous coronary intervention.<\/jats:p>","DOI":"10.3390\/ijms19082306","type":"journal-article","created":{"date-parts":[[2018,8,7]],"date-time":"2018-08-07T03:44:18Z","timestamp":1533613458000},"page":"2306","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["A Short Half-Life \u03b1IIb\u03b23 Antagonist ANTP266 Reduces Thrombus Formation"],"prefix":"10.3390","volume":"19","author":[{"given":"Tong-Dan","family":"Liu","sequence":"first","affiliation":[{"name":"School of Life Science &amp; Technology, China Pharmaceutical University, 24 Tong Jia Street, Nanjing 210009, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shen-Hong","family":"Ren","sequence":"additional","affiliation":[{"name":"School of Life Science &amp; Technology, China Pharmaceutical University, 24 Tong Jia Street, Nanjing 210009, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xue","family":"Ding","sequence":"additional","affiliation":[{"name":"School of Life Science &amp; Technology, China Pharmaceutical University, 24 Tong Jia Street, Nanjing 210009, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhou-Ling","family":"Xie","sequence":"additional","affiliation":[{"name":"School of Pharmacy, China Pharmaceutical University, 24 Tong Jia Street, Nanjing 210009, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2329-7009","authenticated-orcid":false,"given":"Yi","family":"Kong","sequence":"additional","affiliation":[{"name":"School of Life Science &amp; Technology, China Pharmaceutical University, 24 Tong Jia Street, Nanjing 210009, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2018,8,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"568","DOI":"10.1002\/ddr.21116","article-title":"Antithrombotic Therapy for Prevention of Various Thrombotic Diseases","volume":"74","author":"Goto","year":"2013","journal-title":"Drug Dev. 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