{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:46:46Z","timestamp":1787028406996,"version":"build-2736575974"},"reference-count":48,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2018,8,9]],"date-time":"2018-08-09T00:00:00Z","timestamp":1533772800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002855","name":"Ministry of Science and Technology of the People's Republic of China","doi-asserted-by":"publisher","award":["2016YFD0101104"],"award-info":[{"award-number":["2016YFD0101104"]}],"id":[{"id":"10.13039\/501100002855","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Premature senescence greatly affects the yield production and the grain quality in plants, although the molecular mechanisms are largely unknown. Here, we identified a novel rice premature senescence leaf 85 (psl85) mutant from ethyl methane sulfonate (EMS) mutagenesis of cultivar Zhongjian100 (the wild-type, WT). The psl85 mutant presented a distinct dwarfism and premature senescence leaf phenotype, starting from the seedling stage to the mature stage, with decreasing level of chlorophyll and degradation of chloroplast, declined photosynthetic capacity, increased content of malonaldehyde (MDA), upregulated expression of senescence-associated genes, and disrupted reactive oxygen species (ROS) scavenging system. Moreover, endogenous abscisic acid (ABA) level was significantly increased in psl85 at the late aging phase, and the detached leaves of psl85 showed more rapid chlorophyll deterioration than that of WT under ABA treatment, indicating that PSL85 was involved in ABA-induced leaf senescence. Genetic analysis revealed that the premature senescence leaf phenotype was controlled by a single recessive nuclear gene which was finally mapped in a 47 kb region on the short arm of chromosome 7, covering eight candidate open reading frames (ORFs). No similar genes controlling a premature senescence leaf phenotype have been identified in the region, and cloning and functional analysis of the gene is currently underway.<\/jats:p>","DOI":"10.3390\/ijms19082339","type":"journal-article","created":{"date-parts":[[2018,8,9]],"date-time":"2018-08-09T10:36:31Z","timestamp":1533810991000},"page":"2339","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Genetic and Physio-Biochemical Characterization of a Novel Premature Senescence Leaf Mutant in Rice (Oryza sativa L.)"],"prefix":"10.3390","volume":"19","author":[{"given":"Yan","family":"He","sequence":"first","affiliation":[{"name":"State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhihong","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liangjian","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaoqing","family":"Tang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3480-1503","authenticated-orcid":false,"given":"Jian-Li","family":"Wu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2018,8,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"278","DOI":"10.1016\/S1360-1385(00)01655-1","article-title":"Molecular aspects of leaf senescence","volume":"5","author":"Quirino","year":"2000","journal-title":"Trends Plant Sci."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"567","DOI":"10.1111\/j.1365-313X.2005.02399.x","article-title":"Comparative transcriptome analysis reveals significant differences in gene expression and signaling pathways between developmental and dark\/starvation-induced senescence in Arabidopsis","volume":"42","author":"Page","year":"2005","journal-title":"Plant J."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1779","DOI":"10.1105\/TPC.010061","article-title":"ORE9, an F-box protein that regulates leaf senescence in Arabidopsis","volume":"13","author":"Woo","year":"2001","journal-title":"Plant Cell"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"814","DOI":"10.1073\/pnas.0505150103","article-title":"Cytokinin-mediated control of leaf longevity by AHK3 through phosphorylation of ARR2 in Arabidopsis","volume":"103","author":"Kim","year":"2006","journal-title":"Proc. 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