{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T23:18:24Z","timestamp":1780442304420,"version":"3.54.1"},"reference-count":41,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2018,8,28]],"date-time":"2018-08-28T00:00:00Z","timestamp":1535414400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Natural Science Foundation of China and the government of Xinjiang province","award":["U1130302"],"award-info":[{"award-number":["U1130302"]}]},{"name":"Special Prophase Project of the Basic Research of The National Department of Science and Technology","award":["2011CB111501"],"award-info":[{"award-number":["2011CB111501"]}]},{"name":"Doctoral Fund program of Xinjiang Bingtuan","award":["Doctoral Fund program of Xinjiang Bingtuan"],"award-info":[{"award-number":["Doctoral Fund program of Xinjiang Bingtuan"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Altay is a typical fat-tailed sheep breed displaying the unique ability to rapidly mobilize fat, which is vital for maintaining a normal metabolism that facilitates its survival in lengthy winter conditions. However, the physiological, biochemical, and molecular mechanisms underlying fat mobilization remain to be elucidated. In this study, the monitoring of rump fat adipocyte sizes disclosed a positive correlation between cell size and fat deposition ability. In addition, we subjected sheep to persistent starvation to imitate the conditions that trigger rump fat mobilization and screened 112 differentially expressed proteins using the isobaric peptide labeling approach. Notably, increased secretion of leptin and adiponectin activated the key fat mobilization signaling pathways under persistent starvation conditions. Furthermore, the upregulation of resistin (RETN), heat-shock protein 72 (HSP72), and complement factor D (CFD) promoted lipolysis, whereas the downregulation of cell death-inducing DFFA-like effector C (CIDEC) inhibited lipid droplet fusion, and the increase in HSP72 and apolipoprotein AI (Apo-AI) levels activated the body\u2019s stress mechanisms. The synergistic actions of the above hormones, genes, and signaling pathways form a molecular network that functions in improving the adaptability of Altay sheep to extreme environments. Our findings provide a reference for elucidating the complex molecular mechanisms underlying rump fat mobilization.<\/jats:p>","DOI":"10.3390\/ijms19092556","type":"journal-article","created":{"date-parts":[[2018,8,28]],"date-time":"2018-08-28T12:19:06Z","timestamp":1535458746000},"page":"2556","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Comparative Proteome Analysis Reveals Lipid Metabolism-Related Protein Networks in Response to Rump Fat Mobilization"],"prefix":"10.3390","volume":"19","author":[{"given":"Juanjuan","family":"Wang","sequence":"first","affiliation":[{"name":"College of Animal Science and Technology, Shihezi University, Shihezi 832000, China"},{"name":"State Key Laboratory of Sheep Genetic Improvement and Healthy Production, Xinjiang Academy of Agricultural and Reclamation Sciences, Shihezi 832000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengsi","family":"Xu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Sheep Genetic Improvement and Healthy Production, Xinjiang Academy of Agricultural and Reclamation Sciences, Shihezi 832000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinhua","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Sheep Genetic Improvement and Healthy Production, Xinjiang Academy of Agricultural and Reclamation Sciences, Shihezi 832000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinquan","family":"Yang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Sheep Genetic Improvement and Healthy Production, Xinjiang Academy of Agricultural and Reclamation Sciences, Shihezi 832000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Gao","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Sheep Genetic Improvement and Healthy Production, Xinjiang Academy of Agricultural and Reclamation Sciences, Shihezi 832000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Laboratory of Epigenetics, Beijing Institute of Biotechnology, Beijing 100071, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Huang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Sheep Genetic Improvement and Healthy Production, Xinjiang Academy of Agricultural and Reclamation Sciences, Shihezi 832000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengli","family":"Han","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Sheep Genetic Improvement and Healthy Production, Xinjiang Academy of Agricultural and Reclamation Sciences, Shihezi 832000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui","family":"Gao","sequence":"additional","affiliation":[{"name":"The Key Laboratory of Xinjiang Endemic &amp; Ethnic Diseases and Department of Biochemistry, Shihezi University School of Medicine, Shihezi 832000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shangquan","family":"Gan","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Sheep Genetic Improvement and Healthy Production, Xinjiang Academy of Agricultural and Reclamation Sciences, Shihezi 832000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2018,8,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1237","DOI":"10.1007\/s11250-011-9854-9","article-title":"Molecular evidence for fat-tailed sheep domestication","volume":"43","author":"Rocha","year":"2011","journal-title":"Trop. Anim. Health Prod."},{"key":"ref_2","first-page":"38","article-title":"Winter feed for transhumant livestock in China: The Altay experience","volume":"87","author":"Li","year":"1996","journal-title":"World Anim. Rev."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1007\/s00203-011-0757-y","article-title":"Diversity and abundance of the rumen and fecal methanogens in Altay sheep native to Xinjiang and the influence of diversity on methane emissions","volume":"194","author":"Liu","year":"2012","journal-title":"Arch. Microbiol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1080\/10409230591008189","article-title":"Adipose development: From stem cell to adipocyte","volume":"40","author":"Otto","year":"2005","journal-title":"Crit. Rev. Biochem. Mol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1038\/75610","article-title":"Triglycerides and toggling the tummy","volume":"25","author":"Kahn","year":"2002","journal-title":"Nat. Genet."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"531","DOI":"10.1016\/S0092-8674(01)00240-9","article-title":"Obesity and the regulation of energy balance","volume":"104","author":"Spiegelman","year":"2014","journal-title":"Cell"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/B978-0-12-411619-1.00006-9","article-title":"Quantifying size and number of adipocytes in adipose tissue","volume":"537","author":"Parlee","year":"2014","journal-title":"Methods Enzymol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/j.chemphyslip.2015.12.007","article-title":"Impact of phosphatidylcholine liposomes on the compositional changes of VLDL during lipoprotein lipase(LPL)-mediated lipolysis","volume":"195","author":"Cwiklinska","year":"2016","journal-title":"Chem. Phys. Lipids"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"15717","DOI":"10.1074\/jbc.M701329200","article-title":"CCAAT\/enhancer-binding protein beta deletion reduces adiposity, hepatic steatosis, and diabetes in Lepr(db\/db) mice","volume":"282","author":"Rahman","year":"2007","journal-title":"J. Biol. Chem."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1016\/j.bcp.2006.03.023","article-title":"Antagonism of peroxisome proliferator-activated receptor \u03b3 prevents high-fat diet-induced obesity in vivo","volume":"72","author":"Nakano","year":"2006","journal-title":"Biochem. Pharmacol."},{"key":"ref_11","first-page":"505","article-title":"Transcriptional regulation of fatty acid synthase gene by insulin\/glucose, polyunsaturated fatty acid and leptin in hepatocytes and adipocytes in normal and genetically obese rats","volume":"260","author":"Fukuda","year":"1999","journal-title":"FEBS J."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"535","DOI":"10.1073\/pnas.97.2.535","article-title":"Another hormone-sensitive triglyceride lipase in fat cells?","volume":"97","author":"Saltiel","year":"2000","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1007\/s11745-012-3742-6","article-title":"Loss of fat with increased adipose triglyceride lipase-mediated lipolysis in adipose tissue during laying stages in Quail","volume":"48","author":"Yang","year":"2013","journal-title":"Lipids"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"363","DOI":"10.1016\/j.diabet.2014.05.004","article-title":"Adiponectin is expressed in the pancreas of high-fat-diet-fed mice and protects pancreatic endothelial function during the development of type 2 diabetes","volume":"40","author":"Liu","year":"2014","journal-title":"Diabetes Metab."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1016\/j.peptides.2013.08.019","article-title":"Resistin levels are related to fat mass, but not to body mass index in children","volume":"49","author":"Ortega","year":"2013","journal-title":"Peptides"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"819","DOI":"10.1007\/s00018-011-0794-3","article-title":"Ablation of LMO4 in glutamatergic neurons impairs leptin control of fat metabolism","volume":"69","author":"Zhou","year":"2012","journal-title":"Cell Mol. Life Sci."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"783","DOI":"10.1111\/eci.12106","article-title":"Adiponectin, visfatin and regional fat depots in normal weight obese premenopausal women","volume":"43","author":"Miazgowski","year":"2013","journal-title":"Eur. J. Clin. Investig."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"531","DOI":"10.1016\/j.metabol.2010.04.023","article-title":"Body fat changes and activity of tumor necrosis factor \u03b1 system-a 5-year follow-up study","volume":"60","author":"Chudek","year":"2011","journal-title":"Metabolism"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1016\/j.jprot.2017.10.008","article-title":"Comparative proteomic analyses using iTRAQ-labeling provides insights into fiber diversity in sheep and goats","volume":"172","author":"Li","year":"2018","journal-title":"J. Proteom."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1016\/S0022-2275(20)34742-8","article-title":"Effects of cell size and animal age on glucose metabolism in pig adipose tissue","volume":"22","author":"Etherton","year":"1981","journal-title":"J. Lipid Res."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"3184","DOI":"10.2527\/2002.80123184x","article-title":"Feeding intensity and dietary protein level affect adipocyte cellularity and lipogenic capacity of muscle homogenates in growing pigs, without modification of the expression of sterol regulatory element binding protein","volume":"80","author":"Gondret","year":"2002","journal-title":"J. Anim. Sci."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1111\/jne.12155","article-title":"Neuropeptide Y and leptin sensitivity is dependent on diet composition","volume":"26","author":"Eggels","year":"2014","journal-title":"J. Neuroendocrinol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"524","DOI":"10.1038\/nm1029","article-title":"Adiponectin acts in the brain to decrease body weight","volume":"10","author":"Qi","year":"2004","journal-title":"Nat. Med."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1016\/j.jpba.2016.03.027","article-title":"Identification and characterization of in vitro and in vivo generated metabolites of the adiponectin receptor agonists AdipoRon and 112,254","volume":"125","author":"Dib","year":"2016","journal-title":"J. Pharmaceut. Biomed."},{"key":"ref_25","first-page":"4293","article-title":"Expression of genes involved in lipid metabolism correlate with peroxisome proliferator activated receptor gamma expression in human skeletal lmuscle","volume":"85","author":"Lapsys","year":"2000","journal-title":"J. Clin. Endocr. Metab."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1016\/S0739-7240(02)00179-0","article-title":"Comparing mRNA levels of genes encoding leptin, leptin receptor, and lipoprotein lipase between dairy and beef cattle","volume":"23","author":"Ren","year":"2002","journal-title":"Domest. Anim. Endocrin."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"579","DOI":"10.1111\/j.1365-2265.2010.03953.x","article-title":"Apolipoprotein B\/A1 ratio is associated with free androgen index and visceral adiposity and may be an indicator of metabolic syndrome in male children and adolescents","volume":"74","author":"Lee","year":"2011","journal-title":"Clin. Endocrinol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1038\/415339a","article-title":"Leptin stimulates fatty-acid oxidation by activating AMP-activated proteinkinase","volume":"415","author":"Minokoshi","year":"2002","journal-title":"Nature"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1676","DOI":"10.1194\/jlr.M002147","article-title":"Differential roles of Cidea and Cidec in insulin-induced anti-apoptosis and lipid droplet (LD) formation in human adipocytes","volume":"51","author":"Ito","year":"2010","journal-title":"J. Lipid Res."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1450","DOI":"10.1194\/jlr.M012427","article-title":"Differential regulation of CIDEA and CIDEC expression by insulin via Akt1\/2 and JNK2-dependent pathways in human adipocytes","volume":"52","author":"Ito","year":"2011","journal-title":"J. Lipid Res."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1097\/MOL.0b013e328328d0bb","article-title":"CIDE proteins and metabolic disorders","volume":"20","author":"Gong","year":"2009","journal-title":"Curr. Opin. Lipidol."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Kwak, S.J., Hong, S.H., Bajracharya, R., Yang, S.Y., Lee, K.S., and Yu, K. (2013). Drosophila Adiponectin receptor in insulin producing cells regulates glucose and lipid metabolism by controlling insulin secretion. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0068641"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1556\/APhysiol.99.2012.1.6","article-title":"Resistin, visfatin and insulin sensitivity in selected phases of annual training cycle of triathletes","volume":"99","author":"Sliwicka","year":"2012","journal-title":"Acta Physiol. Hung."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1739","DOI":"10.1073\/pnas.0705799105","article-title":"HSP72 protects against obesity-induced insulin resistance","volume":"105","author":"Chung","year":"2008","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"839","DOI":"10.1152\/japplphysiol.00148.2008","article-title":"Age-related differences in skeletal muscle insulin signaling: The role of stress kinases and heat shock proteins","volume":"105","author":"Gupte","year":"2008","journal-title":"J. Appl. Physiol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1978","DOI":"10.1046\/j.1523-1755.2003.00312.x","article-title":"Gene expression profile in diabetic KK\/Ta mice","volume":"64","author":"Fan","year":"2003","journal-title":"Kidney Int."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1726","DOI":"10.1073\/pnas.93.4.1726","article-title":"Decreased food intake does not completely account for adiposity reduction after ob protein infusion","volume":"93","author":"Levin","year":"1996","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"451","DOI":"10.1152\/japplphysiol.00849.2010","article-title":"Acute heat treatment improves insulin-stimulated glucose uptake in aged skeletal muscle","volume":"110","author":"Gupte","year":"2011","journal-title":"J. Appl. Physiol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1097\/JES.0b013e318201f236","article-title":"Heat shock proteins are important mediators of skeletal muscle insulin sensitivity","volume":"39","author":"Geiger","year":"2011","journal-title":"Exerc. Sport Sci. Rev."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1007\/s12192-008-0073-x","article-title":"Inflammation, heat shock proteins, and type 2 diabetes","volume":"14","author":"Hooper","year":"2009","journal-title":"Cell Stress Chaperon."},{"key":"ref_41","first-page":"490","article-title":"Site-specific oxidation of apolipoprotein a-I impairs cholesterol export by ABCA 1, a key cardioprotective function of HDL","volume":"1821","author":"Shao","year":"2012","journal-title":"BBA-Biomembranes"}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/19\/9\/2556\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T15:21:44Z","timestamp":1760196104000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/19\/9\/2556"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,8,28]]},"references-count":41,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2018,9]]}},"alternative-id":["ijms19092556"],"URL":"https:\/\/doi.org\/10.3390\/ijms19092556","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,8,28]]}}}