{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T14:14:53Z","timestamp":1790518493531,"version":"4.1.0"},"reference-count":63,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2018,9,3]],"date-time":"2018-09-03T00:00:00Z","timestamp":1535932800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003329","name":"Ministerio de Econom\u00eda y Competitividad","doi-asserted-by":"publisher","award":["PCIN-2017-055"],"award-info":[{"award-number":["PCIN-2017-055"]}],"id":[{"id":"10.13039\/501100003329","id-type":"DOI","asserted-by":"publisher"}]},{"name":"ERANET-ARIMNet2","award":["EMERAMB (ref 302)"],"award-info":[{"award-number":["EMERAMB (ref 302)"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Tomato yellow leaf curl virus (TYLCV, genus Begomovirus, family Geminiviridae) is a major species that causes a tomato disease for which resistant tomato hybrids (mainly carriers of the Ty-1\/Ty-3 gene) are being used widely. We have characterized begomoviruses severely affecting resistant tomato crops in Southeast Spain. Circular DNA was prepared from samples by rolling circle amplification, and sequenced by massive sequencing (2015) or cloning and Sanger sequencing (2016). Thus, 23 complete sequences were determined, all belonging to the TYLCV Israel strain (TYLCV-IL). Massive sequencing also revealed the absence of other geminiviral and beta-satellite sequences. A phylogenetic analysis showed that the Spanish isolates belonged to two groups, one related to early TYLCV-IL isolates in the area (Group 1), and another (Group 2) closely related to El Jadida (Morocco) isolates, suggesting a recent introduction. The most parsimonious evolutionary scenario suggested that the TYLCV isolates of Group 2 are back recombinant isolates derived from TYLCV-IS76, a recombinant virus currently predominating in Moroccan epidemics. Thus, an infectious Group 2 clone (TYLCV-Mu15) was constructed and used in in planta competition assays against TYLCV-IS76. TYLCV-Mu15 predominated in single infections, whereas TYLCV-IS76 did so in mixed infections, providing credibility to a scenario of co-occurrence of both types of isolates.<\/jats:p>","DOI":"10.3390\/ijms19092614","type":"journal-article","created":{"date-parts":[[2018,9,5]],"date-time":"2018-09-05T03:08:55Z","timestamp":1536116935000},"page":"2614","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Characterization of Begomoviruses Sampled during Severe Epidemics in Tomato Cultivars Carrying the Ty-1 Gene"],"prefix":"10.3390","volume":"19","author":[{"given":"Covadonga","family":"Torre","sequence":"first","affiliation":[{"name":"Abiopep S.L., Departamento de I + D + i, Parque Cient\u00edfico de Murcia, Ctra. de Madrid, Km 388, Complejo de Espinardo, Edf. R, 2\u00b0, 30100 Murcia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Livia","family":"Donaire","sequence":"additional","affiliation":[{"name":"Centro de Edafolog\u00eda y Biolog\u00eda Aplicada del Segura (CEBAS)-CSIC, Departamento de Biolog\u00eda del Estr\u00e9s y Patolog\u00eda Vegetal, P.O. Box 164, 30100 Murcia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristina","family":"G\u00f3mez-Aix","sequence":"additional","affiliation":[{"name":"Abiopep S.L., Departamento de I + D + i, Parque Cient\u00edfico de Murcia, Ctra. de Madrid, Km 388, Complejo de Espinardo, Edf. R, 2\u00b0, 30100 Murcia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miguel","family":"Ju\u00e1rez","sequence":"additional","affiliation":[{"name":"Escuela Polit\u00e9cnica Superior de Orihuela, Universidad Miguel Hern\u00e1ndez de Elche, Ctra. de Beniel, Km 3.2, 03312 Alicante, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michel","family":"Peterschmitt","sequence":"additional","affiliation":[{"name":"Centre de Coop\u00e9ration Internationale en Recherche Agronomique Pour le D\u00e9veloppement (CIRAD), UMR-BGPI, Equipe Interactions Virus-Insecte-Plante, TA A-54\/K, Campus International de Baillarguet, CEDEX 5, 34398 Monptellier, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cica","family":"Urbino","sequence":"additional","affiliation":[{"name":"Centre de Coop\u00e9ration Internationale en Recherche Agronomique Pour le D\u00e9veloppement (CIRAD), UMR-BGPI, Equipe Interactions Virus-Insecte-Plante, TA A-54\/K, Campus International de Baillarguet, CEDEX 5, 34398 Monptellier, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yolanda","family":"Hernando","sequence":"additional","affiliation":[{"name":"Abiopep S.L., Departamento de I + D + i, Parque Cient\u00edfico de Murcia, Ctra. de Madrid, Km 388, Complejo de Espinardo, Edf. R, 2\u00b0, 30100 Murcia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7854-2008","authenticated-orcid":false,"given":"Jes\u00fas","family":"Ag\u00fcero","sequence":"additional","affiliation":[{"name":"Abiopep S.L., Departamento de I + D + i, Parque Cient\u00edfico de Murcia, Ctra. de Madrid, Km 388, Complejo de Espinardo, Edf. R, 2\u00b0, 30100 Murcia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miguel A.","family":"Aranda","sequence":"additional","affiliation":[{"name":"Centro de Edafolog\u00eda y Biolog\u00eda Aplicada del Segura (CEBAS)-CSIC, Departamento de Biolog\u00eda del Estr\u00e9s y Patolog\u00eda Vegetal, P.O. Box 164, 30100 Murcia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2018,9,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/B978-0-12-394314-9.00002-6","article-title":"Major Tomato Viruses in the Mediterranean Basin","volume":"84","author":"Hanssen","year":"2012","journal-title":"Adv. Virus Res."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1016\/S0304-4238(96)00945-4","article-title":"Viral diseases causing the greatest economic losses to the tomato crop. II The Tomato yellow leaf curl virus-a review","volume":"67","author":"Diez","year":"1996","journal-title":"Sci. Hortic."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1593","DOI":"10.1007\/s00705-015-2398-y","article-title":"Revision of Begomovirus taxonomy based on pairwise sequence comparisons","volume":"160","author":"Brown","year":"2015","journal-title":"Arch. 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