{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T17:43:11Z","timestamp":1783705391117,"version":"3.55.0"},"reference-count":40,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2018,9,7]],"date-time":"2018-09-07T00:00:00Z","timestamp":1536278400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000005","name":"U.S. Department of Defense","doi-asserted-by":"publisher","award":["OR130220"],"award-info":[{"award-number":["OR130220"]}],"id":[{"id":"10.13039\/100000005","id-type":"DOI","asserted-by":"publisher"}]},{"name":"LLNL LDRD","award":["16-ERD-007"],"award-info":[{"award-number":["16-ERD-007"]}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["AR062603"],"award-info":[{"award-number":["AR062603"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Anterior cruciate ligament (ACL) injuries often result in post-traumatic osteoarthritis (PTOA). To better understand the molecular mechanisms behind PTOA development following ACL injury, we profiled ACL injury-induced transcriptional changes in knee joints of three mouse strains with varying susceptibility to OA: STR\/ort (highly susceptible), C57BL\/6J (moderately susceptible) and super-healer MRL\/MpJ (not susceptible). Right knee joints of the mice were injured using a non-invasive tibial compression injury model and global gene expression was quantified before and at 1-day, 1-week, and 2-weeks post-injury using RNA-seq. Following injury, injured and uninjured joints of STR\/ort and injured C57BL\/6J joints displayed significant cartilage degeneration while MRL\/MpJ had little cartilage damage. Gene expression analysis suggested that prolonged inflammation and elevated catabolic activity in STR\/ort injured joints, compared to the other two strains may be responsible for the severe PTOA phenotype observed in this strain. MRL\/MpJ had the lowest expression values for several inflammatory cytokines and catabolic enzymes activated in response to ACL injury. Furthermore, we identified several genes highly expressed in MRL\/MpJ compared to the other two strains including B4galnt2 and Tpsab1 which may contribute to enhanced healing in the MRL\/MpJ. Overall, this study has increased our knowledge of early molecular changes associated with PTOA development.<\/jats:p>","DOI":"10.3390\/ijms19092657","type":"journal-article","created":{"date-parts":[[2018,9,7]],"date-time":"2018-09-07T11:47:41Z","timestamp":1536320861000},"page":"2657","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Comparative Transcriptomics Identifies Novel Genes and Pathways Involved in Post-Traumatic Osteoarthritis Development and Progression"],"prefix":"10.3390","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7822-7040","authenticated-orcid":false,"given":"Aimy","family":"Sebastian","sequence":"first","affiliation":[{"name":"Physical and Life Sciences Directorate, Lawrence Livermore National Laboratories, Livermore, CA 95101, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiun C.","family":"Chang","sequence":"additional","affiliation":[{"name":"Physical and Life Sciences Directorate, Lawrence Livermore National Laboratories, Livermore, CA 95101, USA"},{"name":"School of Natural Sciences, UC Merced, Merced, CA 95101, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melanie E.","family":"Mendez","sequence":"additional","affiliation":[{"name":"Physical and Life Sciences Directorate, Lawrence Livermore National Laboratories, Livermore, CA 95101, USA"},{"name":"School of Natural Sciences, UC Merced, Merced, CA 95101, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deepa K.","family":"Murugesh","sequence":"additional","affiliation":[{"name":"Physical and Life Sciences Directorate, Lawrence Livermore National Laboratories, Livermore, CA 95101, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah","family":"Hatsell","sequence":"additional","affiliation":[{"name":"Regeneron Pharmaceuticals, Tarrytown, NY 10020, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aris N.","family":"Economides","sequence":"additional","affiliation":[{"name":"Regeneron Pharmaceuticals, Tarrytown, NY 10020, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Blaine A.","family":"Christiansen","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery, UC Davis Medical Center, Sacramento, CA 95101, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9546-5561","authenticated-orcid":false,"given":"Gabriela G.","family":"Loots","sequence":"additional","affiliation":[{"name":"Physical and Life Sciences Directorate, Lawrence Livermore National Laboratories, Livermore, CA 95101, USA"},{"name":"School of Natural Sciences, UC Merced, Merced, CA 95101, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2018,9,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1038\/nrrheum.2014.44","article-title":"The individual and socioeconomic impact of osteoarthritis","volume":"10","author":"Hunter","year":"2014","journal-title":"Nat. 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