{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T05:46:19Z","timestamp":1789537579191,"version":"build-2803163510"},"reference-count":67,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2018,9,18]],"date-time":"2018-09-18T00:00:00Z","timestamp":1537228800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005732","name":"National Taiwan Ocean University","doi-asserted-by":"publisher","award":["105 J298031"],"award-info":[{"award-number":["105 J298031"]}],"id":[{"id":"10.13039\/501100005732","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Xiamen Medical College","award":["K2016-08"],"award-info":[{"award-number":["K2016-08"]}]},{"DOI":"10.13039\/501100003410","name":"Department of Education, Fujian Province","doi-asserted-by":"publisher","award":["JAT160586"],"award-info":[{"award-number":["JAT160586"]}],"id":[{"id":"10.13039\/501100003410","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>In rats, mice, and humans, it is known that zinc deficiency may be related to anemia, and zinc supplementation influences hemoglobin production. Our previous studies indicate that in fish, zinc supplementation stimulates red blood cell (RBC) formation (erythropoiesis). However, it is not clear whether the mechanism of zinc-induced erythropoiesis stimulation in fish also occurs in rats. We induced anemia in rats using phenylhydrazine (PHZ) and injected either saline or ZnSO4 solution. We found that an appropriate amount of zinc stimulated erythropoiesis in the PHZ-induced anemic rats. The effects of ZnSO4 injection were dose-dependent. When the concentration of ZnSO4 was higher than 2.8 mg zinc\/kg body weight, the RBC level of the anemic rats increased from 60 \u00b1 7% to 88 \u00b1 10% that of the normal rats in two days. Rat bone marrow cells with or without ZnCl2 supplementation were cultured in suspension in vitro. In the cell culture when the zinc concentration was at 0.3 mM, a 1.6-fold proliferation of nascent immature reticulocytes (new RBCs) was observed after one day. In the rat blood, zinc was combined with serum transferrin to induce erythropoiesis. The stimulation of RBC formation by zinc appears to be common among different animals.<\/jats:p>","DOI":"10.3390\/ijms19092824","type":"journal-article","created":{"date-parts":[[2018,9,18]],"date-time":"2018-09-18T11:52:29Z","timestamp":1537271549000},"page":"2824","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":45,"title":["Zinc Supplementation Stimulates Red Blood Cell Formation in Rats"],"prefix":"10.3390","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3576-1371","authenticated-orcid":false,"given":"Yen-Hua","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Basic Medicine, The Center of Translational Medicine, Xiamen Medical College, Xiamen 361023, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui-Lin","family":"Feng","sequence":"additional","affiliation":[{"name":"Department of Food Science, College of Life Sciences, National Taiwan Ocean University, Keelung 20224, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5648-8381","authenticated-orcid":false,"given":"Sen-Shyong","family":"Jeng","sequence":"additional","affiliation":[{"name":"Department of Food Science, College of Life Sciences, National Taiwan Ocean University, Keelung 20224, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2018,9,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1159\/000348547","article-title":"Update on zinc biology","volume":"62","author":"Solomons","year":"2013","journal-title":"Ann Nutr. 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