{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T20:29:55Z","timestamp":1793046595016,"version":"4.5.0"},"reference-count":30,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2018,9,19]],"date-time":"2018-09-19T00:00:00Z","timestamp":1537315200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001677","name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale","doi-asserted-by":"publisher","award":["recurrent funding"],"award-info":[{"award-number":["recurrent funding"]}],"id":[{"id":"10.13039\/501100001677","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004794","name":"Centre National de la Recherche Scientifique","doi-asserted-by":"publisher","award":["recurrent funding"],"award-info":[{"award-number":["recurrent funding"]}],"id":[{"id":"10.13039\/501100004794","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005413","name":"Universit\u00e9 Paris Descartes","doi-asserted-by":"publisher","award":["recurrent funding"],"award-info":[{"award-number":["recurrent funding"]}],"id":[{"id":"10.13039\/501100005413","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008966","name":"Soci\u00e9t\u00e9 Francophone du Diab\u00e8te","doi-asserted-by":"publisher","award":["Allocation SFD-Industrie"],"award-info":[{"award-number":["Allocation SFD-Industrie"]}],"id":[{"id":"10.13039\/501100008966","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","award":["2010 BLAN 1123 01"],"award-info":[{"award-number":["2010 BLAN 1123 01"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]},{"name":"CORDDIM R\u00e9gion Ile de France","award":["Allocation Recherche"],"award-info":[{"award-number":["Allocation Recherche"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The energy sensor AMP-activated protein kinase (AMPK) is a key player in the control of energy metabolism. AMPK regulates hepatic lipid metabolism through the phosphorylation of its well-recognized downstream target acetyl CoA carboxylase (ACC). Although AMPK activation is proposed to lower hepatic triglyceride (TG) content via the inhibition of ACC to cause inhibition of de novo lipogenesis and stimulation of fatty acid oxidation (FAO), its contribution to the inhibition of FAO in vivo has been recently questioned. We generated a mouse model of AMPK activation specifically in the liver, achieved by expression of a constitutively active AMPK using adenoviral delivery. Indirect calorimetry studies revealed that liver-specific AMPK activation is sufficient to induce a reduction in the respiratory exchange ratio and an increase in FAO rates in vivo. This led to a more rapid metabolic switch from carbohydrate to lipid oxidation during the transition from fed to fasting. Finally, mice with chronic AMPK activation in the liver display high fat oxidation capacity evidenced by increased [C14]-palmitate oxidation and ketone body production leading to reduced hepatic TG content and body adiposity. Our findings suggest a role for hepatic AMPK in the remodeling of lipid metabolism between the liver and adipose tissue.<\/jats:p>","DOI":"10.3390\/ijms19092826","type":"journal-article","created":{"date-parts":[[2018,9,19]],"date-time":"2018-09-19T10:50:31Z","timestamp":1537354231000},"page":"2826","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":133,"title":["AMPK Activation Reduces Hepatic Lipid Content by Increasing Fat Oxidation In Vivo"],"prefix":"10.3390","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7017-9032","authenticated-orcid":false,"given":"Marc","family":"Foretz","sequence":"first","affiliation":[{"name":"INSERM, U1016, Institut Cochin, D\u00e9partement d\u2019Endocrinologie M\u00e9tabolisme et Diab\u00e8te, 24, rue du Faubourg Saint Jacques, 75014 Paris, France"},{"name":"CNRS, UMR8104, 75014 Paris, France"},{"name":"Universit\u00e9 Paris Descartes, Sorbonne Paris Cit\u00e9, 75014 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5733-3023","authenticated-orcid":false,"given":"Patrick C.","family":"Even","sequence":"additional","affiliation":[{"name":"UMR PNCA, AgroParisTech, INRA, Universit\u00e9 Paris-Saclay, 75005 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0121-0224","authenticated-orcid":false,"given":"Benoit","family":"Viollet","sequence":"additional","affiliation":[{"name":"INSERM, U1016, Institut Cochin, D\u00e9partement d\u2019Endocrinologie M\u00e9tabolisme et Diab\u00e8te, 24, rue du Faubourg Saint Jacques, 75014 Paris, France"},{"name":"CNRS, UMR8104, 75014 Paris, France"},{"name":"Universit\u00e9 Paris Descartes, Sorbonne Paris Cit\u00e9, 75014 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2018,9,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1146\/annurev-nutr-071812-161148","article-title":"AMP-activated protein kinase: Maintaining energy homeostasis at the cellular and whole-body levels","volume":"34","author":"Hardie","year":"2014","journal-title":"Annu. 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