{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,16]],"date-time":"2026-05-16T20:21:29Z","timestamp":1778962889158,"version":"3.51.4"},"reference-count":68,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2018,9,20]],"date-time":"2018-09-20T00:00:00Z","timestamp":1537401600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["x"],"award-info":[{"award-number":["x"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["x"],"award-info":[{"award-number":["x"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004901","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de Minas Gerais","doi-asserted-by":"publisher","award":["x"],"award-info":[{"award-number":["x"]}],"id":[{"id":"10.13039\/501100004901","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Previous studies showed that P1G10, a proteolytic fraction from Vasconcellea cundinamarcensis latex, reduced the tumor mass in animals bearing melanoma, increased in vitro DNA fragmentation and decreased cell adhesion. Here, we present some molecular and cellular events related to the antimetastatic effect induced by the CMS-2 fraction derived from P1G10 in metastatic melanoma B16-F10 and melanocyte Melan-a. Using difference gel electrophoresis and mass spectrometry, we identified four proteins overexpressed in tumor cells, all of them related to proliferation, survival, migration and cell invasion, that had their expression normalized upon treatment with CMS-2: nucleophosmin 1, heat shock protein 65, calcyclin binding protein and eukaryotic translation initiation factor 4H. In addition, some antioxidant and glycolytic enzymes show increased expression after exposure to CMS-2, along with an induction of melanogenesis (differentiation marker). The down regulation of cofilin 1, a protein involved in cell motility, may explain the inhibition of cell migration and dendritic-like outgrowth in B16-F10 and Melan-a, observed after CMS-2 treatment. Taken together, it is argued that CMS-2 modulates the expression of proteins related to metastatic development, driving the cell to a more differentiated-like state. These effects support the CMS-2 antimetastatic activity and place this fraction in the category of anticancer agent.<\/jats:p>","DOI":"10.3390\/ijms19102846","type":"journal-article","created":{"date-parts":[[2018,9,21]],"date-time":"2018-09-21T03:46:35Z","timestamp":1537501595000},"page":"2846","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Cysteine Proteases from V. cundinamarcensis (C. candamarcensis) Inhibit Melanoma Metastasis and Modulate Expression of Proteins Related to Proliferation, Migration and Differentiation"],"prefix":"10.3390","volume":"19","author":[{"given":"Fernanda O.","family":"Lemos","sequence":"first","affiliation":[{"name":"Department of Pharmacology, Biological Science Institute, Federal University of Minas Gerais-UFMG, Av. Ant\u00f4nio Carlos 6627, Belo Horizonte 31270-901, MG, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4370-0803","authenticated-orcid":false,"given":"Dalton","family":"Dittz","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Biological Science Institute, Federal University of Minas Gerais-UFMG, Av. Ant\u00f4nio Carlos 6627, Belo Horizonte 31270-901, MG, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Verlane G.","family":"Santos","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Biological Science Institute, Federal University of Minas Gerais-UFMG, Av. Ant\u00f4nio Carlos 6627, Belo Horizonte 31270-901, MG, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Simone F.","family":"Pires","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Biological Science Institute, Federal University of Minas Gerais-UFMG, Av. Ant\u00f4nio Carlos 6627, Belo Horizonte 31270-901, MG, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H\u00e9lida M.","family":"De Andrade","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Biological Science Institute, Federal University of Minas Gerais-UFMG, Av. Ant\u00f4nio Carlos 6627, Belo Horizonte 31270-901, MG, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlos E.","family":"Salas","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Immunology, Biological Science Institute, Federal University of Minas Gerais-UFMG, Av. Ant\u00f4nio Carlos 6627, Belo Horizonte 31270-901, MG, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miriam T. P.","family":"Lopes","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Biological Science Institute, Federal University of Minas Gerais-UFMG, Av. Ant\u00f4nio Carlos 6627, Belo Horizonte 31270-901, MG, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2018,9,20]]},"reference":[{"key":"ref_1","unstructured":"Le\u00f3n Hurtado, J. (1987). Bot\u00e1nica de Los Cultivos Tropicales, IICA. [2nd ed.]."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"e175","DOI":"10.1016\/j.thromres.2013.01.028","article-title":"The thrombolytic action of a proteolytic fraction (p1g10) from Carica candamarcensis","volume":"131","author":"Bilheiro","year":"2013","journal-title":"Thromb. 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