{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T17:18:36Z","timestamp":1773422316853,"version":"3.50.1"},"reference-count":66,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2018,9,27]],"date-time":"2018-09-27T00:00:00Z","timestamp":1538006400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000055","name":"National Institute on Deafness and Other Communication Disorders","doi-asserted-by":"publisher","award":["R01DC012831"],"award-info":[{"award-number":["R01DC012831"]}],"id":[{"id":"10.13039\/100000055","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Functional maintenance of the mammalian main olfactory epithelium (MOE) is challenging because of its direct exposure to a wide spectrum of environmental chemicals. We previously reported that transient receptor potential channel M5-expressing microvillous cells (TRPM5-MCs) in the MOE play an important role in olfactory maintenance. To investigate the underpinning mechanisms, we exposed transcription factor Skn-1a knockout (Skn-1a\u2212\/\u2212) mice lacking TRPM5-MCs, and TRPM5-GFP mice to either vehicle (water) or a mixture of odorous chemicals and chitin for two weeks and analyzed the expression of olfactory signaling proteins using immunolabeling and neurotrophin (NT) and NT receptor (NTR) gene transcripts using real-time quantitative PCR. The chemical exposure did not significantly attenuate the immunolabeling of olfactory signaling proteins. Vehicle-exposed Skn-1a\u2212\/\u2212 and TRPM5-GFP mice expressed similar levels of NT and NTR gene transcripts in the MOE and olfactory bulb. Chemical exposure significantly increased MOE expression of p75NTR in Skn-1a\u2212\/\u2212 mice, while p75NTR expression was reduced in TRPM5-GFP mice, as compared to vehicle-exposed mice. Additionally, our RNA in situ hybridization analysis and immunolabeling confirmed MOE expression of most NTs and NTRs. Together, these results indicate that TRPM5-MCs and chemical exposure influence expression of some NTs and NTRs in the MOE and olfactory bulb (OB).<\/jats:p>","DOI":"10.3390\/ijms19102939","type":"journal-article","created":{"date-parts":[[2018,9,28]],"date-time":"2018-09-28T02:54:54Z","timestamp":1538103294000},"page":"2939","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Chemical Exposure-Induced Changes in the Expression of Neurotrophins and Their Receptors in the Main Olfactory System of Mice Lacking TRPM5-Expressing Microvillous Cells"],"prefix":"10.3390","volume":"19","author":[{"given":"Abdullah","family":"AlMatrouk","sequence":"first","affiliation":[{"name":"Department of Biological Sciences, University of Maryland, Baltimore County, Baltimore, MD 21250, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kayla","family":"Lemons","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, University of Maryland, Baltimore County, Baltimore, MD 21250, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tatsuya","family":"Ogura","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, University of Maryland, Baltimore County, Baltimore, MD 21250, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wangmei","family":"Luo","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, University of Maryland, Baltimore County, Baltimore, MD 21250, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chantel","family":"Wilson","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, University of Maryland, Baltimore County, Baltimore, MD 21250, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Weihong","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, University of Maryland, Baltimore County, Baltimore, MD 21250, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2018,9,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1683","DOI":"10.1289\/EHP136","article-title":"Effects of Ambient Air Pollution Exposure on Olfaction: A Review","volume":"124","author":"Ajmani","year":"2016","journal-title":"Environ. 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