{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T03:47:03Z","timestamp":1790394423621,"version":"4.1.0"},"reference-count":92,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2018,10,8]],"date-time":"2018-10-08T00:00:00Z","timestamp":1538956800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31570533;31872356"],"award-info":[{"award-number":["31570533;31872356"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Sperm cryopreservation and artificial insemination are important methods for giant panda breeding and preservation of extant genetic diversity. Lower conception rates limit the use of artificial insemination with frozen-thawed giant panda sperm, due to the lack of understanding of the cryodamaging or cryoinjuring mechanisms in cryopreservation. Long non-coding RNAs (lncRNAs) are involved in regulating spermatogenesis. However, their roles during cryopreservation remain largely unexplored. Therefore, this study aimed to identify differentially expressed lncRNAs and mRNAs associated with cryodamage or freeze tolerance in frozen-thawed sperm through high throughput sequencing. A total of 61.05 Gb clean reads and 22,774 lncRNA transcripts were obtained. From the sequencing results, 1477 significantly up-regulated and 1,396 significantly down-regulated lncRNA transcripts from fresh and frozen-thawed sperm of giant panda were identified. GO and KEGG showed that the significantly dysregulated lncRNAs and mRNAs were mainly involved in regulating responses to cold stress and apoptosis, such as the integral component of membrane, calcium transport, and various signaling pathways including PI3K-Akt, p53 and cAMP. Our work is the first systematic profiling of lncRNA and mRNA in fresh and frozen-thawed giant panda sperm, and provides valuableinsights into the potential mechanism of cryodamage in sperm.<\/jats:p>","DOI":"10.3390\/ijms19103066","type":"journal-article","created":{"date-parts":[[2018,10,8]],"date-time":"2018-10-08T10:44:53Z","timestamp":1538995493000},"page":"3066","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":33,"title":["Transcriptome Sequencing Reveals the Differentially Expressed lncRNAs and mRNAs Involved in Cryoinjuries in Frozen-Thawed Giant Panda (Ailuropoda melanoleuca) Sperm"],"prefix":"10.3390","volume":"19","author":[{"given":"Ming-Xia","family":"Ran","sequence":"first","affiliation":[{"name":"College of Animal Sciences and Technology, Sichuan Agricultural University, Chengdu 611130, Sichuan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuan","family":"Li","sequence":"additional","affiliation":[{"name":"College of Animal Sciences and Technology, Sichuan Agricultural University, Chengdu 611130, Sichuan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Animal Sciences and Technology, Sichuan Agricultural University, Chengdu 611130, Sichuan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kai","family":"Liang","sequence":"additional","affiliation":[{"name":"College of Animal Sciences and Technology, Sichuan Agricultural University, Chengdu 611130, Sichuan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying-Nan","family":"Ren","sequence":"additional","affiliation":[{"name":"College of Animal Sciences and Technology, Sichuan Agricultural University, Chengdu 611130, Sichuan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Animal Sciences and Technology, Sichuan Agricultural University, Chengdu 611130, Sichuan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4493-8311","authenticated-orcid":false,"given":"Guang-Bin","family":"Zhou","sequence":"additional","affiliation":[{"name":"College of Animal Sciences and Technology, Sichuan Agricultural University, Chengdu 611130, Sichuan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying-Min","family":"Zhou","sequence":"additional","affiliation":[{"name":"China Conservation and Research Center for the Giant Panda, Wolong 473000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kai","family":"Wu","sequence":"additional","affiliation":[{"name":"China Conservation and Research Center for the Giant Panda, Wolong 473000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheng-Dong","family":"Wang","sequence":"additional","affiliation":[{"name":"China Conservation and Research Center for the Giant Panda, Wolong 473000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Huang","sequence":"additional","affiliation":[{"name":"China Conservation and Research Center for the Giant Panda, Wolong 473000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo","family":"Luo","sequence":"additional","affiliation":[{"name":"China Conservation and Research Center for the Giant Panda, Wolong 473000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3052-0109","authenticated-orcid":false,"given":"Izhar","family":"Qazi","sequence":"additional","affiliation":[{"name":"College of Animal Sciences and Technology, Sichuan Agricultural University, Chengdu 611130, Sichuan, China"},{"name":"Department of Veterinary Anatomy &amp; Histology, Faculty of Bio-Sciences, Shaheed Benazir Bhutto University of Veterinary and Animal Sciences, Sakrand 67210, Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"He-Min","family":"Zhang","sequence":"additional","affiliation":[{"name":"China Conservation and Research Center for the Giant Panda, Wolong 473000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9797-4551","authenticated-orcid":false,"given":"Chang-Jun","family":"Zeng","sequence":"additional","affiliation":[{"name":"College of Animal Sciences and Technology, Sichuan Agricultural University, Chengdu 611130, Sichuan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2018,10,8]]},"reference":[{"key":"ref_1","first-page":"27","article-title":"The artificial breeding and protecrion of giant panda","volume":"11","author":"Feng","year":"1992","journal-title":"Explor. 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