{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T10:40:44Z","timestamp":1762252844477,"version":"build-2065373602"},"reference-count":44,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2018,10,17]],"date-time":"2018-10-17T00:00:00Z","timestamp":1539734400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministry of Health and Welfare","award":["HN14C0086"],"award-info":[{"award-number":["HN14C0086"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Melanogenesis is a biological process resulting in the production of melanin pigment, which plays an important role in the prevention of sun-induced skin injury, and determines hair and skin color. So, a wide variety of approaches have been proposed to increase the synthesis of melanin. This study evaluated the effects of pulsed electromagnetic fields (PEMFs) on the pigmentation of zebrafish (Danio rerio) in vivo. We stimulated pigmentation in zebrafish by using specific frequencies and intensities of PEMFs. This study focuses on pigmentation using PEMFs, and finds that PEMFs, at an optimal intensity and frequency, upregulate pigmentation by the stimulated expression of tyrosinase-related protein 1 (TRP1), dopachrome tautomerase (DCT) through extracellular signal-regulated kinase(ERK) phosphorylation, and p38 phosphorylation signaling pathways in zebrafish. These results suggest that PEMFs, at an optimal intensity and frequency, are a useful tool in treating gray hair, with reduced melanin synthesis in the hair shaft or hypopigmentation-related skin disorders.<\/jats:p>","DOI":"10.3390\/ijms19103211","type":"journal-article","created":{"date-parts":[[2018,10,17]],"date-time":"2018-10-17T10:22:54Z","timestamp":1539771774000},"page":"3211","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Pulsed Electromagnetic Fields Increase Pigmentation through the p-ERK\/p-p38 Pathway in Zebrafish (Danio rerio)"],"prefix":"10.3390","volume":"19","author":[{"given":"Yu-Mi","family":"Kim","sequence":"first","affiliation":[{"name":"Department of Medical Biotechnology (BK21 Plus Team), Dongguk University, Goyang-si 10326, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Han-Moi","family":"Lim","sequence":"additional","affiliation":[{"name":"Department of Medical Biotechnology (BK21 Plus Team), Dongguk University, Goyang-si 10326, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hyang-Seon","family":"Ro","sequence":"additional","affiliation":[{"name":"R&amp;D Center, NOWCOS, Seoul 08506, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ga-Eun","family":"Ki","sequence":"additional","affiliation":[{"name":"Department of Medical Biotechnology (BK21 Plus Team), Dongguk University, Goyang-si 10326, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7533-9605","authenticated-orcid":false,"given":"Young-Kwon","family":"Seo","sequence":"additional","affiliation":[{"name":"Department of Medical Biotechnology (BK21 Plus Team), Dongguk University, Goyang-si 10326, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2018,10,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1038\/emm.2001.23","article-title":"Stimulation of melanogenesis by glycyrrhizin in B16 melanoma cells","volume":"33","author":"Jung","year":"2001","journal-title":"Exp. 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