{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,18]],"date-time":"2026-07-18T15:18:48Z","timestamp":1784387928032,"version":"3.55.0"},"reference-count":54,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2018,10,18]],"date-time":"2018-10-18T00:00:00Z","timestamp":1539820800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001824","name":"Grantov\u00e1 Agentura \u010cesk\u00e9 Republiky","doi-asserted-by":"publisher","award":["GACR GB14-37368G"],"award-info":[{"award-number":["GACR GB14-37368G"]}],"id":[{"id":"10.13039\/501100001824","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011659","name":"L\u00e9ka\u0159sk\u00e1 fakulta, Masarykova univerzita","doi-asserted-by":"publisher","award":["MUNI\/A\/1008\/2017"],"award-info":[{"award-number":["MUNI\/A\/1008\/2017"]}],"id":[{"id":"10.13039\/501100011659","id-type":"DOI","asserted-by":"publisher"}]},{"name":"funds from the Faculty of Medicine of Masaryk University awarded to junior researcher Petra Borilova Linhartova.","award":["without number"],"award-info":[{"award-number":["without number"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Chronic periodontitis (CP) and diabetes mellitus (DM) involve several aspects of immune functions, including neutrophil activity and cytokine biology. Considering the critical function of chemokine interleukin-8 (IL-8) in the inflammatory process, the aims of this study were to determine: (i) IL-8 plasma levels; (ii) IL-8 (\u2212251A\/T, rs4073) and its receptor 2 (CXCR2, +1208C\/T, rs1126579) polymorphisms, and (iii) the presence of the selected periodontal bacteria in types 1 and 2 DM patients (T1DM and T2DM) and systemically healthy controls (HC) with known periodontal status. This case\u2013control study comprises of 153 unrelated individuals: 36\/44 patients suffering from T1DM+CP\/T2DM+CP and 32\/41 from HC+CP\/non-periodontitis HC. Both the clinical and biochemical parameters were monitored. The genotypes were determined using qPCR, IL-8 plasma levels were measured using an ELISA kit. Subgingival bacterial colonization was analyzed with a DNA microarray detection kit. The IL-8 plasma levels differed significantly between non-periodontitis HC and T1DM+CP\/T2DM+CP patients (P &lt; 0.01). Even in HC+CP, IL-8 concentrations were significantly lower than in T1DM+CP\/T2DM+CP patients (P \u2264 0.05). No significant associations between the IL-8 plasma levels and the studied IL-8 and CXCR2 polymorphisms or the occurrence of selected periodontal bacteria (P &gt; 0.05) were found. CP does not influence the circulating IL-8 levels. Patients with T1DM+CP\/T2DM+CP had higher circulating IL-8 levels than HC+CP\/non-periodontitis HC.<\/jats:p>","DOI":"10.3390\/ijms19103214","type":"journal-article","created":{"date-parts":[[2018,10,18]],"date-time":"2018-10-18T10:55:41Z","timestamp":1539860141000},"page":"3214","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":34,"title":["Differences in Interleukin-8 Plasma Levels between Diabetic Patients and Healthy Individuals Independently on Their Periodontal Status"],"prefix":"10.3390","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0953-3615","authenticated-orcid":false,"given":"Petra","family":"Borilova Linhartova","sequence":"first","affiliation":[{"name":"Clinic of Stomatology, Institution Shared with St. Anne\u2019s Faculty Hospital, Faculty of Medicine, Masaryk University, Pekarska 664\/53, 60200 Brno, Czech Republic"},{"name":"Department of Pathophysiology, Faculty of Medicine, Masaryk University, Kamenice 5, 62500 Brno, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8476-8401","authenticated-orcid":false,"given":"Denisa","family":"Kavrikova","sequence":"additional","affiliation":[{"name":"Clinic of Stomatology, Institution Shared with St. Anne\u2019s Faculty Hospital, Faculty of Medicine, Masaryk University, Pekarska 664\/53, 60200 Brno, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie","family":"Tomandlova","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Faculty of Medicine, Masaryk University, Kamenice 5, 62500 Brno, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hana","family":"Poskerova","sequence":"additional","affiliation":[{"name":"Clinic of Stomatology, Institution Shared with St. Anne\u2019s Faculty Hospital, Faculty of Medicine, Masaryk University, Pekarska 664\/53, 60200 Brno, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vaclav","family":"Rehka","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Masaryk University, Kamenice 5, 62500 Brno, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ladislav","family":"Du\u0161ek","sequence":"additional","affiliation":[{"name":"Institute of Biostatistics and Analyses, Faculty of Medicine, Masaryk University, 62500 Brno, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7610-8929","authenticated-orcid":false,"given":"Lydie","family":"Izakovicova Holla","sequence":"additional","affiliation":[{"name":"Clinic of Stomatology, Institution Shared with St. Anne\u2019s Faculty Hospital, Faculty of Medicine, Masaryk University, Pekarska 664\/53, 60200 Brno, Czech Republic"},{"name":"Department of Pathophysiology, Faculty of Medicine, Masaryk University, Kamenice 5, 62500 Brno, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2018,10,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1016\/j.diabres.2016.11.022","article-title":"Call to action: A new path for improving diabetes care for Indigenous peoples, a global review","volume":"123","author":"Harris","year":"2017","journal-title":"Diabetes Res. Clin. Pract."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1254","DOI":"10.2522\/ptj.20080020","article-title":"Epidemiology of diabetes and diabetes-related complications","volume":"88","author":"Deshpande","year":"2008","journal-title":"Phys. Ther."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.mvr.2014.06.010","article-title":"Role of advanced glycation end product (AGE)-induced receptor (RAGE) expression in diabetic vascular complications","volume":"95","author":"Chawla","year":"2014","journal-title":"Microvasc. Res."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"189525","DOI":"10.1155\/2015\/189525","article-title":"Complications of diabetes","volume":"2015","author":"Papatheodorou","year":"2015","journal-title":"J. Diabetes Res."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"329","DOI":"10.2337\/diacare.16.1.329","article-title":"Periodontal disease. The sixth complication of diabetes mellitus","volume":"16","year":"1993","journal-title":"Diabetes Care"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"182","DOI":"10.2174\/1573399812666161220144011","article-title":"Systemic cytokines in type 2 diabetes mellitus and chronic periodontitis","volume":"14","author":"Acharya","year":"2018","journal-title":"Curr. Diabetes Rev."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"926","DOI":"10.1902\/annals.1996.1.1.926","article-title":"Consensus report. Periodontal diseases: Pathogenesis and microbial factors","volume":"1","author":"Genco","year":"1996","journal-title":"Ann. Periodontol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1111\/j.1600-0757.2010.00357.x","article-title":"Comparison of the microbiological features of chronic and aggressive periodontitis","volume":"53","author":"Armitage","year":"2010","journal-title":"Periodontol. 2000"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"22962","DOI":"10.3402\/jom.v6.22962","article-title":"Genetic dysbiosis: The role of microbial insults in chronic inflammatory diseases","volume":"6","author":"Nibali","year":"2014","journal-title":"J. Oral Microbiol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1161\/01.CIR.102.1.42","article-title":"Chronic subclinical inflammation as part of the insulin resistance syndrome: The Insulin Resistance Atherosclerosis Study (IRAS)","volume":"102","author":"Festa","year":"2000","journal-title":"Circulation"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1196\/annals.1366.032","article-title":"Low-grade inflammation in chronic infectious diseases: Paradigm of periodontal infections","volume":"1088","author":"Moutsopoulos","year":"2006","journal-title":"Ann. N. Y. Acad. Sci."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"410","DOI":"10.1902\/jop.1981.52.8.410","article-title":"Comparison of neutrophil chemotactic response in diabetic patients with mild and severe periodontal disease","volume":"52","author":"Spagnuolo","year":"1981","journal-title":"J. Periodontol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1046\/j.1432-1327.1998.2550067.x","article-title":"Differential usage of the CXC chemokine receptors 1 and 2 by interleukin-8, granulocyte chemotactic protein-2 and epithelial-cell-derived neutrophil attractant-78","volume":"255","author":"Wuyts","year":"1998","journal-title":"Eur. J. Biochem."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1002\/jlb.56.5.559","article-title":"Essential involvement of interleukin-8 (IL-8) in acute inflammation","volume":"56","author":"Harada","year":"1994","journal-title":"J. Leukoc. Biol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"364","DOI":"10.1007\/s10753-012-9555-1","article-title":"CXCL10 activities, biological structure, and source along with its significant role played in pathophysiology of type I diabetes mellitus","volume":"36","author":"Ahmadi","year":"2013","journal-title":"Inflammation"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"641","DOI":"10.1515\/jpem-2015-0340","article-title":"Monocyte chemoattractant protein 1 and macrophage migration inhibitory factor in children with type 1 diabetes","volume":"29","author":"Ismail","year":"2016","journal-title":"J. Pediatr. Endocrinol. Metab."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"467","DOI":"10.1055\/s-0032-1331226","article-title":"Reduced blood leukocyte and neutrophil numbers in the pathogenesis of type 1 diabetes","volume":"45","author":"Harsunen","year":"2013","journal-title":"Horm. Metab. Res."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"2072","DOI":"10.2337\/db12-1345","article-title":"Reduction of circulating neutrophils precedes and accompanies type 1 diabetes","volume":"62","author":"Valle","year":"2013","journal-title":"Diabetes"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1329","DOI":"10.2337\/db14-0443","article-title":"CXCR1\/2 inhibition blocks and reverses type 1 diabetes in mice","volume":"64","author":"Citro","year":"2015","journal-title":"Diabetes"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Haurogn\u00e9, K., Pavlovic, M., Rogniaux, H., Bach, J.M., and Lieubeau, B. (2015). Type 1 diabetes prone NOD mice have diminished Cxcr1 mRNA expression in polymorphonuclear neutrophils and CD4+ T lymphocytes. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0134365"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1249","DOI":"10.1902\/jop.2015.150149","article-title":"The influence of glycated hemoglobin on the cross susceptibility between type 1 diabetes mellitus and periodontal disease","volume":"86","author":"Lappin","year":"2015","journal-title":"J. Periodontol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"578","DOI":"10.1111\/odi.12502","article-title":"High glucose-induced oxidative stress increases IL-8 production in human gingival epithelial cells","volume":"22","author":"Kashiwagi","year":"2016","journal-title":"Oral Dis."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"784","DOI":"10.1111\/j.1600-051X.2006.00984.x","article-title":"The influence of diabetes on gingival crevicular fluid beta-glucuronidase and interleukin-8","volume":"33","author":"Engebretson","year":"2006","journal-title":"J. Clin. Periodontol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"449","DOI":"10.4103\/1735-3327.166193","article-title":"Inflammatory markers in gingival crevicular fluid of periodontitis patients with type 2 diabetes mellitus according to glycemic control: A pilot study","volume":"12","author":"Longo","year":"2015","journal-title":"Dent. Res. J. (Isfahan)"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Mohamed, H.G., Idris, S.B., Ahmed, M.F., \u00c5str\u00f8m, A.N., Mustafa, K., Ibrahim, S.O., and Mustafa, M. (2015). Influence of type 2 diabetes on local production of inflammatory molecules in adults with and without chronic periodontitis: A cross-sectional study. BMC Oral Health, 15.","DOI":"10.1186\/s12903-015-0073-z"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"E1179","DOI":"10.1210\/JC.2015-1388","article-title":"Large-scale discovery and validation studies demonstrate significant reductions in circulating levels of IL8, IL-1Ra, MCP-1, and MIP-1\u03b2 in patients with type 1 diabetes","volume":"100","author":"Purohit","year":"2015","journal-title":"J. Clin. Endocrinol. Metab."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1023","DOI":"10.1136\/thorax.55.12.1023","article-title":"Association of respiratory syncytial virus bronchiolitis with the interleukin 8 gene region in UK families","volume":"55","author":"Hull","year":"2000","journal-title":"Thorax"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"R201","DOI":"10.1186\/ar3534","article-title":"Decreased CXCR1 and CXCR2 expression on neutrophils in anti-neutrophil cytoplasmic autoantibody-associated vasculitides potentially increases neutrophil adhesion and impairs migration","volume":"13","author":"Hu","year":"2011","journal-title":"Arthritis Res. Ther."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"566","DOI":"10.1158\/0008-5472.CAN-14-2101","article-title":"Identification of a functional SNP in the 3\u2019UTR of CXCR2 that is associated with reduced risk of lung cancer","volume":"75","author":"Ryan","year":"2015","journal-title":"Cancer Res."},{"key":"ref_30","first-page":"342351","article-title":"Haplotype analysis of interleukin-8 gene polymorphisms in chronic and aggressive periodontitis","volume":"2013","author":"Vokurka","year":"2013","journal-title":"Mediat. Inflamm."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"S106","DOI":"10.1902\/jop.2013.1340011","article-title":"Diabetes and periodontal diseases: Consensus report of the Joint EFP\/AAP Workshop on Periodontitis and Systemic Diseases","volume":"84","author":"Chapple","year":"2013","journal-title":"J. Periodontol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"S135","DOI":"10.1902\/jop.2013.1340013","article-title":"Effect of periodontal disease on diabetes: Systematic review of epidemiologic observational evidence","volume":"84","author":"Borgnakke","year":"2013","journal-title":"J. Periodontol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1902\/annals.2001.6.1.125","article-title":"Periodontitis and diabetes interrelationships: Role of inflammation","volume":"6","author":"Iacopino","year":"2001","journal-title":"Ann. Periodontol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1016\/j.cyto.2009.08.011","article-title":"Increased circulating IL-8 is associated with reduced IGF-1 and related to poor metabolic control in adolescents with type 1 diabetes mellitus","volume":"48","author":"Simmons","year":"2009","journal-title":"Cytokine"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1902\/jop.2016.160140","article-title":"Influence of periodontal disease on changes of glycated hemoglobin levels in type 2 diabetics: A retrospective cohort study","volume":"88","author":"Costa","year":"2017","journal-title":"J. Periodontol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1111\/jcpe.12803","article-title":"An update on the evidence for pathogenic mechanisms that may link periodontitis and diabetes","volume":"45","author":"Polak","year":"2018","journal-title":"J. Clin. Periodontol."},{"key":"ref_37","first-page":"230","article-title":"Plasma level of interleukin-6 and interleukin-8 in the elderly","volume":"59","author":"Korybalska","year":"2002","journal-title":"Prz. Lek."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1038\/sj.ijo.0803259","article-title":"Circulating levels of MCP-1 and IL-8 are elevated in human obese subjects and associated with obesity-related parameters","volume":"30","author":"Kim","year":"2006","journal-title":"Int. J. Obes. (Lond.)"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"552","DOI":"10.1016\/j.cyto.2012.06.239","article-title":"Association of matrix metalloproteinase (MMP)-1, 3, 9, interleukin (IL)-2, 8 and cyclooxygenase (COX)-2 gene polymorphisms with chronic periodontitis in a Chinese population","volume":"60","author":"Li","year":"2012","journal-title":"Cytokine"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1192","DOI":"10.1902\/jop.2012.110584","article-title":"Effect of priming in subpopulations of peripheral neutrophils from patients with chronic periodontitis","volume":"83","author":"Fredriksson","year":"2012","journal-title":"J. Periodontol."},{"key":"ref_41","first-page":"256","article-title":"Serum inflammatory and immune mediators are elevated in early stage diabetic nephropathy","volume":"45","author":"Perlman","year":"2015","journal-title":"Ann. Clin. Lab. Sci."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"6431","DOI":"10.1158\/1078-0432.CCR-05-0942","article-title":"The -251T allele of the interleukin-8 promoter is associated with increased risk of gastric carcinoma featuring diffuse-type histopathology in Chinese population","volume":"11","author":"Lee","year":"2005","journal-title":"Clin. Cancer Res."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"31180","DOI":"10.1038\/srep31180","article-title":"Investigation of the functional role of human Interleukin-8 gene haplotypes by CRISPR\/Cas9 mediated genome editing","volume":"6","author":"Benakanakere","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"889","DOI":"10.1111\/jcpe.12600","article-title":"Periodontal infectogenomics: Systematic review of associations between host genetic variants and subgingival microbial detection","volume":"43","author":"Nibali","year":"2016","journal-title":"J. Clin. Periodontol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"e000260","DOI":"10.1136\/bmjdrc-2016-000260","article-title":"Systemic inflammatory responses in patients with type 2 diabetes with chronic periodontitis","volume":"4","author":"Mesia","year":"2016","journal-title":"BMJ Open Diabetes Res. Care"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1111\/j.1600-0765.2007.00974.x","article-title":"Innate immune responses of gingival epithelial cells to nonperiodontopathic and periodontopathic bacteria","volume":"42","author":"Ji","year":"2007","journal-title":"J. Periodontal. Res."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"380","DOI":"10.1902\/jop.2016.160212","article-title":"Strain-specific impact of Fusobacterium nucleatum on neutrophil function","volume":"88","author":"Kurgan","year":"2017","journal-title":"J. Periodontol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"85757","DOI":"10.1155\/2014\/185757","article-title":"The effect of IL-4 gene polymorphisms on cytokine production in patients with chronic periodontitis and in healthy controls","volume":"2014","author":"Bartova","year":"2014","journal-title":"Mediat. Inflamm."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1902\/annals.1999.4.1.1","article-title":"Development of a classification system for periodontal diseases and conditions","volume":"4","author":"Armitage","year":"1999","journal-title":"Ann. Periodontol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"456","DOI":"10.1016\/j.archoralbio.2014.10.003","article-title":"Apolipoprotein E gene polymorphisms in relation to chronic periodontitis, periodontopathic bacteria, and lipid levels","volume":"60","author":"Bartova","year":"2015","journal-title":"Arch. Oral Biol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"610","DOI":"10.1902\/jop.1967.38.6.610","article-title":"The gingival index, the plaque index and the retention index systems","volume":"38","year":"1967","journal-title":"J. Periodontol."},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"American Diabetes Association (2016). Standards of Medical Care in Diabetes\u20142016. Classification and Diagnosis of Diabetes. Diabetes Care, 39, S11\u2013S22.","DOI":"10.2337\/dc16-S005"},{"key":"ref_53","unstructured":"Czech Diabetes Association (2017, July 22). Diabetic Treatment. (In Czech)."},{"key":"ref_54","unstructured":"Sambrook, J., Fritsch, E.F., and Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press. [2nd ed.]."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/19\/10\/3214\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T15:26:23Z","timestamp":1760196383000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/19\/10\/3214"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,10,18]]},"references-count":54,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2018,10]]}},"alternative-id":["ijms19103214"],"URL":"https:\/\/doi.org\/10.3390\/ijms19103214","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,10,18]]}}}