{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T04:11:44Z","timestamp":1769573504965,"version":"3.49.0"},"reference-count":44,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2018,10,22]],"date-time":"2018-10-22T00:00:00Z","timestamp":1540166400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Trihexanoin is a short-chain triglyceride (SCT). Many studies have reported that SCTs play important roles in the maintenance of intestinal epithelial structure and function. The present work was to investigate the effects of trihexanoin on growth performance, carbohydrate and fat metabolism, as well as intestinal morphology and function in weaned piglets. Twenty weaned piglets (21 \u00b1 2 d) were randomly allocated to one of two treatment groups: The control group (basal diet supplemented with 0.5% soya oil); the TH group (basal diet supplemented with 0.5% trihexanoin). Dietary trihexanoin supplementation significantly reduced diarrhea rate; increased the concentrations of LDL, HDL and total protein in plasma; decreased cholesterol concentrations and glutamyl transpeptidase activity in plasma; improved intestinal morphologic structure; altered the mRNA levels and abundances of proteins related to glycogen and fat metabolism, mucosal barrier function, antioxidant capacity and water transport capacity; and altered the community of intestinal microflora. These results indicate that dietary trihexanoin supplementation could reduce diarrhea, regulate carbohydrate and fat metabolism, exert beneficial effects on the intestinal mucosal barrier, protect the intestinal mucosa from injuries, improve intestinal transport and absorption, and enhance antioxidant capacity. In conclusion, dietary supplementation with 0.5% trihexanoin improves the intestinal function and health of weaned piglets.<\/jats:p>","DOI":"10.3390\/ijms19103277","type":"journal-article","created":{"date-parts":[[2018,10,23]],"date-time":"2018-10-23T08:43:36Z","timestamp":1540284216000},"page":"3277","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Dietary Supplementation with Trihexanoin Enhances Intestinal Function of Weaned Piglets"],"prefix":"10.3390","volume":"19","author":[{"given":"Tao","family":"Wu","sequence":"first","affiliation":[{"name":"Engineering Research Center of Feed Protein Resources on Agricultural By-products, Ministry of Education, Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kang","family":"Li","sequence":"additional","affiliation":[{"name":"Engineering Research Center of Feed Protein Resources on Agricultural By-products, Ministry of Education, Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dan","family":"Yi","sequence":"additional","affiliation":[{"name":"Engineering Research Center of Feed Protein Resources on Agricultural By-products, Ministry of Education, Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lei","family":"Wang","sequence":"additional","affiliation":[{"name":"Engineering Research Center of Feed Protein Resources on Agricultural By-products, Ministry of Education, Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Di","family":"Zhao","sequence":"additional","affiliation":[{"name":"Engineering Research Center of Feed Protein Resources on Agricultural By-products, Ministry of Education, Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6009-303X","authenticated-orcid":false,"given":"Yang","family":"Lv","sequence":"additional","affiliation":[{"name":"Engineering Research Center of Feed Protein Resources on Agricultural By-products, Ministry of Education, Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lin","family":"Zhang","sequence":"additional","affiliation":[{"name":"Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongbo","family":"Chen","sequence":"additional","affiliation":[{"name":"Engineering Research Center of Feed Protein Resources on Agricultural By-products, Ministry of Education, Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Binying","family":"Ding","sequence":"additional","affiliation":[{"name":"Engineering Research Center of Feed Protein Resources on Agricultural By-products, Ministry of Education, Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yongqing","family":"Hou","sequence":"additional","affiliation":[{"name":"Engineering Research Center of Feed Protein Resources on Agricultural By-products, Ministry of Education, Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guoyao","family":"Wu","sequence":"additional","affiliation":[{"name":"Engineering Research Center of Feed Protein Resources on Agricultural By-products, Ministry of Education, Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China"},{"name":"Department of Animal Science, Texas A&amp;M University, College Station, TX 77843, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2018,10,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1017\/S000711450720580X","article-title":"Main intestinal markers associated with the changes in gut architecture and function in piglets after weaning","volume":"97","author":"Montagne","year":"2007","journal-title":"Br. J. Nutr."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1012","DOI":"10.2527\/jas.2015-9481","article-title":"Supplementation of piglets with nutrient-dense complex milk replacer improves intestinal development and microbial fermentation","volume":"94","author":"De","year":"2016","journal-title":"J. Anim. Sci."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1016\/j.aninu.2017.06.003","article-title":"Weaning stress and gastrointestinal barrier development: Implications for lifelong gut health in pig","volume":"3","author":"Moeser","year":"2017","journal-title":"Anim. Nutr."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1134","DOI":"10.1017\/S1751731107000560","article-title":"Intestinal development and growth performance of early-weaned piglets fed a low-threonine diet","volume":"1","author":"Hamard","year":"2007","journal-title":"Animal"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"442","DOI":"10.1021\/jf00038a038","article-title":"Random nature of triacylglycerols produced by the catalyzed interesterification of short- and long-chain fatty acid triglycerides","volume":"42","author":"Klemann","year":"1994","journal-title":"J. Agric. Food Chem."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1079\/BJN19940123","article-title":"Osmotic pressure, water kinetics and volatile fatty acid absorption in the rumen of sheep sustained by intragastric infusions","volume":"71","author":"Hovell","year":"1994","journal-title":"Br. J. Nutr."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1282","DOI":"10.1071\/AN14285","article-title":"Characterising barrier function among regions of the gastrointestinal tract in Holstein steers","volume":"54","author":"Penner","year":"2014","journal-title":"Anim. Prod. Sci."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1619","DOI":"10.3945\/jn.109.104638","article-title":"Butyrate Enhances the Intestinal Barrier by Facilitating Tight Junction Assembly via Activation of AMP-Activated Protein Kinase in Caco-2 Cell Monolayers","volume":"139","author":"Peng","year":"2009","journal-title":"J. Nutr."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"420","DOI":"10.1016\/j.anaerobe.2010.05.006","article-title":"Bacterial, SCFA and gas profiles of a range of food ingredients following in vitro fermentation by human colonic microbiota","volume":"16","author":"Beards","year":"2010","journal-title":"Anaerobe"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1177\/0148607194018003208","article-title":"Effects of the short-chain triglyceride triacetin on intestinal mucosa and metabolic substrates in rats","volume":"18","author":"Lynch","year":"1994","journal-title":"J. Parent. Enter. Nutr."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"954","DOI":"10.1093\/ajcn\/53.4.954","article-title":"Experimental short-bowel syndrome: Effect of an elemental diet supplemented with short-chain triglycerides","volume":"53","author":"Kripke","year":"1991","journal-title":"Am. J. Clin. Nutr."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"836","DOI":"10.2527\/jas.2006-822","article-title":"Evaluation of alternatives to antibiotics using an Escherichia coli K88+ model of piglet diarrhea: Effects on gut microbial ecology","volume":"86","author":"Bhandari","year":"2008","journal-title":"J. Anim. Sci."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"431","DOI":"10.1084\/jem.94.5.431","article-title":"The Dominant Role of the Liver in Plasma Protein Synthesis","volume":"94","author":"Miller","year":"1951","journal-title":"J. Exp. Med."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"37","DOI":"10.3109\/07420529309064480","article-title":"Circannual Variations in Blood Cholesterol Levels","volume":"10","author":"Harari","year":"1993","journal-title":"Chronobiol. Int."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"224","DOI":"10.1007\/s12011-010-8714-1","article-title":"Manganese supplementation reduces the blood cholesterol levels in Ca-deficient ovariectomized rats","volume":"141","author":"Bae","year":"2011","journal-title":"Biol. Trace Elem. Res."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"21435","DOI":"10.1016\/S0021-9258(17)31822-7","article-title":"Interaction of gamma-glutamyl transpeptidase with acivicin","volume":"269","author":"Stole","year":"1994","journal-title":"J. Biol. Chem."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"S4","DOI":"10.1016\/S1096-6374(12)60013-6","article-title":"Insulin receptor (INSR) and insulin-like growth factor-I receptor (IGF-GR) translocate to nucleus and regulate IGF-GR gene expression in breast cancer cells","volume":"22","author":"Sarfstein","year":"2012","journal-title":"Growth Horm. Igf. Res."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"10723","DOI":"10.1021\/jf2025659","article-title":"Profiling of hepatic gene expression of mice fed with edible japanese mushrooms by DNA microarray analysis: Comparison among Pleurotus ostreatus, Grifola frondosa, and Hypsizigus marmoreus","volume":"59","author":"Sato","year":"2011","journal-title":"J. Agric. Food Chem."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Wu, G. (2018). Principles of Animal Nutrition, CRC Press.","DOI":"10.1201\/9781315120065"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1016\/j.canlet.2016.11.028","article-title":"Argininosuccinate synthetase 1 (ASS1) is a common metabolic marker of chemosensitivity for targeted arginine- and glutamine-starvation therapy","volume":"388","author":"Long","year":"2017","journal-title":"Cancer Lett."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1373","DOI":"10.1001\/archinte.167.13.1373","article-title":"Cholesterol Fractions and Apolipoproteins as Risk Factors for Heart Disease Mortality in Older Men","volume":"167","author":"Clarke","year":"2007","journal-title":"Arch. Intern. Med."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1161\/01.CIR.79.1.8","article-title":"High-density lipoprotein cholesterol and cardiovascular disease. Four prospective American studies","volume":"79","author":"Gordon","year":"1989","journal-title":"Circulation"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1829","DOI":"10.1194\/jlr.M800198-JLR200","article-title":"Hormone-sensitive lipase is involved in hepatic cholesteryl ester hydrolysis","volume":"49","author":"Sekiya","year":"2008","journal-title":"J. Lipid Res."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"4631","DOI":"10.1016\/S0021-9258(20)64369-1","article-title":"Mammalian adipose tissue and muscle are major sources of lipid transfer protein mRNA","volume":"266","author":"Jiang","year":"1991","journal-title":"J. Biol. Chem."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"339","DOI":"10.3389\/fphys.2012.00339","article-title":"A Systems Biology Approach to Deciphering the Etiology of Steatosis Employing Patient-Derived Dermal Fibroblasts and iPS Cells","volume":"3","author":"Jozefczuk","year":"2012","journal-title":"Front. Physiol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"21779","DOI":"10.1074\/jbc.M401071200","article-title":"HFA1 encoding an organelle-specific acetyl-CoA carboxylase controls mitochondrial fatty acid synthesis in Saccharomyces cerevisiae","volume":"279","author":"Hoja","year":"2004","journal-title":"J. Biol. Chem."},{"key":"ref_27","first-page":"9","article-title":"Fatty Acid Synthase (FASN) expression in Triple-Negative Breast Cancer","volume":"72","author":"Oliveras","year":"2012","journal-title":"Cancer Res."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1169","DOI":"10.5713\/ajas.2007.1169","article-title":"Isolation and Cloning of Porcine SLC27A2 Gene and Detection of Its Polymorphism Associated with Growth and Carcass Traits","volume":"20","author":"Wang","year":"2007","journal-title":"Asian Austral. J. Anim. Sci."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1233","DOI":"10.1007\/s00726-011-1191-9","article-title":"Protective effects of N-acetylcysteine on intestinal functions of piglets challenged with lipopolysaccharide","volume":"43","author":"Hou","year":"2012","journal-title":"Amino Acids"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"431","DOI":"10.1002\/iub.175","article-title":"Regulation and Roles for Claudin-family Tight Junction Proteins","volume":"61","author":"Findley","year":"2009","journal-title":"IUBMB Life"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"2011","DOI":"10.1128\/JVI.01888-08","article-title":"Tight Junction Proteins Claudin-1 and Occludin Control Hepatitis C Virus Entry and Are Downregulated during Infection to Prevent Superinfection","volume":"83","author":"Liu","year":"2009","journal-title":"J. Virol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1213","DOI":"10.1152\/ajpcell.00558.2003","article-title":"The tight junction: A multifunctional complex","volume":"286","author":"Schneeberger","year":"2004","journal-title":"Am. J. Physiol. Cell Physiol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1002\/jcb.24308","article-title":"Hyperosmotic induction of aquaporin expression in rat astrocytes through a different MAPK pathway","volume":"114","author":"Yang","year":"2013","journal-title":"J. Cell. Biochem."},{"key":"ref_34","first-page":"19","article-title":"Impaired expression of glutathione synthetic enzyme genes in mice with targeted deletion of the Nrf2 basic-leucine zipper protein","volume":"1517","author":"Chan","year":"2000","journal-title":"BBA Biomembr."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1106","DOI":"10.1161\/HYPERTENSIONAHA.109.140087","article-title":"Genetic silencing of NOX2 and Nox4 reveals differential roles of these NADPH oxidase homologues in the vasopressor and dipsogenic effects of brain angiotensin-II","volume":"54","author":"Peterson","year":"2009","journal-title":"Hypertension"},{"key":"ref_36","first-page":"115","article-title":"Arsenic, GSTO2 Asp142 polymorphism, health and treatment","volume":"7","author":"Masoudi","year":"2008","journal-title":"Excli. J."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"e01824-17","DOI":"10.1128\/mBio.01824-17","article-title":"Different Substrate Preferences Help Closely Related Bacteria to Coexist in the Gut","volume":"8","author":"Louis","year":"2017","journal-title":"mBio"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/S0301-6226(97)00057-2","article-title":"Factors influencing the structure and function of the small intestine in the weaned pig: A review","volume":"51","author":"Pluske","year":"1997","journal-title":"Livest. Prod. Sci."},{"key":"ref_39","first-page":"2","article-title":"Intestinal flora and human health","volume":"5","author":"Mitsuoka","year":"1996","journal-title":"Asia Pac. J. Clin. Nutr."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1128\/jb.162.1.42-46.1985","article-title":"Involvement of autolysin in cellular lysis of Bacillus subtilis induced by short- and medium-chain fatty acids","volume":"162","author":"Tsuchido","year":"1985","journal-title":"J. Bacteriol."},{"key":"ref_41","first-page":"301","article-title":"Activity of the amoxicillin-clavulanic acid (augmentin) combination on strains of hospital isolates","volume":"33","author":"Deforges","year":"1985","journal-title":"Pathol. Biol."},{"key":"ref_42","first-page":"B847","article-title":"Identification of enterococcus species isolated from clinical specimens and detection of vancomycin resistant Enterococci","volume":"5","author":"Ujjwala","year":"2014","journal-title":"Int. J. Pharm. Bio. Sci."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"4390","DOI":"10.1128\/AEM.00260-08","article-title":"Genetic Homogeneity of Clostridium botulinum Type A1 Strains with Unique Toxin Gene Clusters","volume":"74","author":"Raphael","year":"2008","journal-title":"Appl. Environ. Microb."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"555","DOI":"10.1007\/s00726-010-0473-y","article-title":"Dietary \u03b1-ketoglutarate supplementation ameliorates intestinal injury in lipopolysaccharide-challenged piglets","volume":"29","author":"Hou","year":"2010","journal-title":"Amino Acids"}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/19\/10\/3277\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T15:25:20Z","timestamp":1760196320000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/19\/10\/3277"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,10,22]]},"references-count":44,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2018,10]]}},"alternative-id":["ijms19103277"],"URL":"https:\/\/doi.org\/10.3390\/ijms19103277","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,10,22]]}}}