{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,11]],"date-time":"2026-07-11T01:31:02Z","timestamp":1783733462953,"version":"3.55.0"},"reference-count":44,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2018,11,2]],"date-time":"2018-11-02T00:00:00Z","timestamp":1541116800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Psoralea corylifolia L., (P. corylifolia), which is used for treating vitiligo in clinic, shows inhibitory and activating effects on tyrosinase, a rate-limiting enzyme of melanogenesis. This study aimed to determine the active ingredients in the ethenal extracts of P. corylifolia on tyrosinase activity. The spectrum-effect relationship and knock-out method were established to predict the active compounds. Their structures were then identified with the high resolution mass spectra. A high performance liquid chromatography method was established to obtain the specific chromatograms. Tyrosinase activity in vitro was assayed by the method of oxidation rate of levodopa. Partial least squares method was used to test the spectrum-effect relationships. Chromatographic peaks P2, P4, P9, P10, P11, P13, P21, P26, P28, and P30 were positively related to the activating effects on tyrosinase activity in PE, whereas chromatographic peaks P1, P3, P6, P14, P16, P19, P22, and P29 were negatively related to the activating effects on tyrosinase in the P. corylifolia (PEs). When the sample concentration was 0.5 g\u00b7mL\u22121, equal to the amount of raw medicinal herbs, the target components were daidzein (P2), psoralen (P5), neobavaisoflavone (P13), and psoralidin (P20), which were consistent with the results of spectrum-effect relationships.<\/jats:p>","DOI":"10.3390\/ijms19113439","type":"journal-article","created":{"date-parts":[[2018,11,5]],"date-time":"2018-11-05T04:26:39Z","timestamp":1541391999000},"page":"3439","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":36,"title":["Screening the Marker Components in Psoralea corylifolia L. with the Aids of Spectrum-Effect Relationship and Component Knock-Out by UPLC-MS2"],"prefix":"10.3390","volume":"19","author":[{"given":"Mengjun","family":"Shi","sequence":"first","affiliation":[{"name":"National Center for Research and Development of Edible Fungus Processing Technology, Henan University, Kaifeng 475004, China"},{"name":"Kaifeng Key Laboratory of Functional Components in Health Food, Kaifeng 475004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Zhang","sequence":"additional","affiliation":[{"name":"National Center for Research and Development of Edible Fungus Processing Technology, Henan University, Kaifeng 475004, China"},{"name":"Hebei Food Inspection and Research Institute, Shijiazhuang 050091, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miaomiao","family":"Song","sequence":"additional","affiliation":[{"name":"National Center for Research and Development of Edible Fungus Processing Technology, Henan University, Kaifeng 475004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong","family":"Sun","sequence":"additional","affiliation":[{"name":"National Center for Research and Development of Edible Fungus Processing Technology, Henan University, Kaifeng 475004, China"},{"name":"Beijing Academy of Food Sciences, China Meat Research Center, Beijing 100068, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changqin","family":"Li","sequence":"additional","affiliation":[{"name":"National Center for Research and Development of Edible Fungus Processing Technology, Henan University, Kaifeng 475004, China"},{"name":"Kaifeng Key Laboratory of Functional Components in Health Food, Kaifeng 475004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1822-6249","authenticated-orcid":false,"given":"Wenyi","family":"Kang","sequence":"additional","affiliation":[{"name":"National Center for Research and Development of Edible Fungus Processing Technology, Henan University, Kaifeng 475004, China"},{"name":"Kaifeng Key Laboratory of Functional Components in Health Food, Kaifeng 475004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2018,11,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"324","DOI":"10.1111\/pcmr.12355","article-title":"Increased circulating Th17 cells and elevated serum levels of TGF-\u03b2 and IL-21 are correlated with human non-segmental vitiligo development","volume":"28","author":"Zhou","year":"2015","journal-title":"Pigment. 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