{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,15]],"date-time":"2026-04-15T21:40:41Z","timestamp":1776289241029,"version":"3.50.1"},"reference-count":48,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2018,12,8]],"date-time":"2018-12-08T00:00:00Z","timestamp":1544227200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>A newly described \u03b2-carbonic anhydrase (CA, EC 4.2.1.1) from the pathogenic protozoan Entamoeba histolytica, EhiCA, was recently shown to possess a significant catalytic activity for the physiologic CO2 hydration reaction (kcat of 6.7 \u00d7 105 s\u22121 and a kcat\/Km of 8.9 \u00d7 107 M\u22121 s\u22121). A panel of sulfonamides and one sulfamate, some of which are clinically used drugs, were investigated for their inhibitory properties against EhiCA. The best inhibitors detected in the study were 4-hydroxymethyl\/ethyl-benzenesulfonamide (KIs of 36\u201389 nM), whereas some sulfanilyl-sulfonamides showed activities in the range of 285\u2013331 nM. Acetazolamide, methazolamide, ethoxzolamide, and dichlorophenamide were less effective inhibitors (KIs of 509\u2013845 nM) compared to other sulfonamides investigated here. As \u03b2-CAs are not present in vertebrates, the present study may be useful for detecting lead compounds for the design of more effective inhibitors with potential to develop anti-infectives with alternative mechanisms of action.<\/jats:p>","DOI":"10.3390\/ijms19123946","type":"journal-article","created":{"date-parts":[[2018,12,10]],"date-time":"2018-12-10T03:36:41Z","timestamp":1544413001000},"page":"3946","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Sulfonamide Inhibition Studies of a New \u03b2-Carbonic Anhydrase from the Pathogenic Protozoan Entamoeba histolytica"],"prefix":"10.3390","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0107-2682","authenticated-orcid":false,"given":"Silvia","family":"Bua","sequence":"first","affiliation":[{"name":"Sezione di Scienze Farmaceutiche e Nutraceutiche, Neurofarba Dept., Universit\u00e0 degli Studi di Firenze, Via U. Schiff 6, 50019 Sesto Fiorentino, Florence, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3833-6629","authenticated-orcid":false,"given":"Susanna","family":"Haapanen","sequence":"additional","affiliation":[{"name":"Faculty of Medicine and Health Technology, Tampere University, 33100 Tampere, Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marianne","family":"Kuuslahti","sequence":"additional","affiliation":[{"name":"Faculty of Medicine and Health Technology, Tampere University, 33100 Tampere, Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7323-8536","authenticated-orcid":false,"given":"Seppo","family":"Parkkila","sequence":"additional","affiliation":[{"name":"Faculty of Medicine and Health Technology, Tampere University, 33100 Tampere, Finland"},{"name":"Fimlab Ltd., Tampere University Hospital, 33100 Tampere, Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4262-0323","authenticated-orcid":false,"given":"Claudiu T.","family":"Supuran","sequence":"additional","affiliation":[{"name":"Sezione di Scienze Farmaceutiche e Nutraceutiche, Neurofarba Dept., Universit\u00e0 degli Studi di Firenze, Via U. Schiff 6, 50019 Sesto Fiorentino, Florence, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2018,12,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"563","DOI":"10.1186\/s13071-018-3147-3","article-title":"Molecular detection and genotyping of pathogenic protozoan parasites in raw and treated water samples from southwest Colombia","volume":"11","author":"Galeano","year":"2018","journal-title":"Parasit. Vectors"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Domazetovska, A., Lee, R., Adhikari, C., Watts, M., Gilroy, N., Stark, D., and Sivagnanam, S. (2018). A 12-Year Retrospective Study of Invasive Amoebiasis in Western Sydney: Evidence of Local Acquisition. Trop. Med. Infect. 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