{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T08:48:17Z","timestamp":1790671697874,"version":"4.1.0"},"reference-count":47,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2018,12,11]],"date-time":"2018-12-11T00:00:00Z","timestamp":1544486400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001870","name":"Fundacja na rzecz Nauki Polskiej","doi-asserted-by":"publisher","award":["HOMING PLUS\/2010-2\/1,"],"award-info":[{"award-number":["HOMING PLUS\/2010-2\/1,"]}],"id":[{"id":"10.13039\/501100001870","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Ceramide accumulation in muscle and in liver is implicated in the induction of insulin resistance. Much less in known about the role of ceramide in adipose tissue. The aim of the present study was to elucidate the role of ceramide in adipose tissue and to clarify whether lipids participate in the regulation of adipocytokine secretion. The experiments were performed on male Wistar rats divided into three groups: 1. Control, 2. fed high fat diet (HFD), and 3. fed HFD and treated with myriocin. Ceramide (Cer) and diacylglycerol (DAG) content were analyzed by LC\/MS\/MS. Hormone sensitive lipase (HSL) phosphorylation was analyzed by Western Blot. Plasma adiponectin and tumor necrosis factor alpha (TNF-\u03b1) concentration were measured by enzyme-linked immunosorbent assay. An oral glucose tolerance test (OGTT) and insulin tolerance test (ITT) was also performed. In HFD group, total DAG and Cer content was elevated in both subcutaneous and visceral adipose tissue, which was accompanied by increased glucose, insulin, and HOMA-IR value. Myriocin treatment restored HOMA-IR as well as glucose and insulin concentration to control values. Moreover, myriocin decreased not only Cer, but also DAG levels in both fat depots. Furthermore, we observed a strong correlation between adiponectin (negative) and TNF-\u03b1 (positive) and Cer in both fat tissues, which suggests that Cer is involved in the regulation of adipocytokine secretion.<\/jats:p>","DOI":"10.3390\/ijms19123995","type":"journal-article","created":{"date-parts":[[2018,12,12]],"date-time":"2018-12-12T03:27:49Z","timestamp":1544585269000},"page":"3995","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":36,"title":["Inhibition of Ceramide De Novo Synthesis Affects Adipocytokine Secretion and Improves Systemic and Adipose Tissue Insulin Sensitivity"],"prefix":"10.3390","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8055-0576","authenticated-orcid":false,"given":"Agnieszka U.","family":"Blachnio-Zabielska","sequence":"first","affiliation":[{"name":"Department of Hygiene, Epidemiology and Metabolic Disorders, Medical University of Bialystok, 15-089 Bialystok, Poland"},{"name":"Department of Physiology, Medical University of Bialystok, 15-089 Bialystok, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0838-3478","authenticated-orcid":false,"given":"Hady","family":"Razak Hady","sequence":"additional","affiliation":[{"name":"1st Department of General Surgery and Endocrinology, Medical University Bialystok, 15-276 Bialystok, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2115-5549","authenticated-orcid":false,"given":"Adam R.","family":"Markowski","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine and Gastroenterology, Polish Red Cross Memorial Municipal Hospital, 15-003 Bialystok, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3395-7357","authenticated-orcid":false,"given":"Adam","family":"Kurianiuk","sequence":"additional","affiliation":[{"name":"Department of Hygiene, Epidemiology and Metabolic Disorders, Medical University of Bialystok, 15-089 Bialystok, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8077-9602","authenticated-orcid":false,"given":"Alicja","family":"Karwowska","sequence":"additional","affiliation":[{"name":"Department of Hygiene, Epidemiology and Metabolic Disorders, Medical University of Bialystok, 15-089 Bialystok, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"G\u00f3rski","sequence":"additional","affiliation":[{"name":"Department of Physiology, Medical University of Bialystok, 15-089 Bialystok, Poland"},{"name":"Department of Basic Sciences, Faculty of Health Sciences, Lomza State University of Applied Sciences, 18-400 Lomza, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Piotr","family":"Zabielski","sequence":"additional","affiliation":[{"name":"Department of Physiology, Medical University of Bialystok, 15-089 Bialystok, Poland"},{"name":"Department of Medical Biology, Medical University of Bialystok, 15-089 Bialystok, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2018,12,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"278","DOI":"10.1210\/er.2002-0010","article-title":"Insulin resistance and chronic cardiovascular inflammatory syndrome","volume":"24","author":"Ricart","year":"2003","journal-title":"Endocr. 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