{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T20:12:01Z","timestamp":1777493521782,"version":"3.51.4"},"reference-count":33,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2018,12,12]],"date-time":"2018-12-12T00:00:00Z","timestamp":1544572800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001711","name":"Schweizerischer Nationalfonds zur F\u00f6rderung der Wissenschaftlichen Forschung","doi-asserted-by":"publisher","award":["130680, 139226, 147697\/1"],"award-info":[{"award-number":["130680, 139226, 147697\/1"]}],"id":[{"id":"10.13039\/501100001711","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Calretinin (CR; CALB2) belonging to the family of EF-hand Ca2+-binding proteins (CaBP) is widely used as a positive marker for the identification of human malignant mesothelioma (MM) and functionally was suggested to play a critical role during carcinogenesis of this highly aggressive asbestos-associated neoplasm. Increasing evidence suggests that CR not only acts as a prototypical Ca2+ buffer protein, i.e., limiting the amplitude of Ca2+ signals but also as a Ca2+ sensor. No studies have yet investigated whether other closely related CaBPs might serve as substitutes for CR\u2019s functions(s) in MM cells. Genetically modified MM cell lines with medium (MSTO-211H and ZL5) or low (SPC111) endogenous CR expression levels were generated that overexpress either CR\u2019s closest homologue calbindin-D28k (CB) or parvalbumin (PV), the latter considered as a \u201cpure\u201d Ca2+ buffer protein. After lentiviral shCALB2-mediated CR downregulation, in both MSTO-211H and ZL5 cells expressing CB or PV, the CR deficiency-mediated increase in cell death was not prevented by CB or PV. With respect to proliferation and cell morphology of SPC111 cells, CB was able to substitute for CR, but not for CR\u2019s other functions to promote cell migration or invasion. In conclusion, CR has a likely unique role in MM that cannot be substituted by \u201csimilar\u201d CaBPs.<\/jats:p>","DOI":"10.3390\/ijms19124015","type":"journal-article","created":{"date-parts":[[2018,12,12]],"date-time":"2018-12-12T10:54:26Z","timestamp":1544612066000},"page":"4015","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Calretinin Functions in Malignant Mesothelioma Cells Cannot Be Replaced by the Closely Related Ca2+-Binding Proteins Calbindin-D28k and Parvalbumin"],"prefix":"10.3390","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2487-7029","authenticated-orcid":false,"given":"Janine","family":"W\u00f6rthm\u00fcller","sequence":"first","affiliation":[{"name":"Anatomy, Section of Medicine, University of Fribourg, Route Albert-Gockel 1, 1700 Fribourg, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anne","family":"Oberson","sequence":"additional","affiliation":[{"name":"Anatomy, Section of Medicine, University of Fribourg, Route Albert-Gockel 1, 1700 Fribourg, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Val\u00e9rie","family":"Salicio","sequence":"additional","affiliation":[{"name":"Anatomy, Section of Medicine, University of Fribourg, Route Albert-Gockel 1, 1700 Fribourg, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Walter","family":"Blum","sequence":"additional","affiliation":[{"name":"Anatomy, Section of Medicine, University of Fribourg, Route Albert-Gockel 1, 1700 Fribourg, Switzerland"},{"name":"Genetica AG, 8001 Zurich, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8277-7555","authenticated-orcid":false,"given":"Beat","family":"Schwaller","sequence":"additional","affiliation":[{"name":"Anatomy, Section of Medicine, University of Fribourg, Route Albert-Gockel 1, 1700 Fribourg, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2018,12,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/978-94-007-2888-2_1","article-title":"The Regulation of a Cell\u2019s Ca2+ Signaling Toolkit: The Ca2+ Homeostasome","volume":"Volume 740","author":"Schwaller","year":"2012","journal-title":"Advances in Experimental Medicine and Biology"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1007\/s00018-008-8564-6","article-title":"The continuing disappearance of \u201cpure\u201d Ca2+ buffers","volume":"66","author":"Schwaller","year":"2009","journal-title":"Cell. 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