{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T20:19:15Z","timestamp":1790108355810,"version":"4.0.1"},"reference-count":57,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2018,12,20]],"date-time":"2018-12-20T00:00:00Z","timestamp":1545264000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The aim of our study was to examine the effects of different dietary strategies, high-fat (HFd) or standard diet (Sd) alone or in combination with standardized oral supplementation (0.45 mL\/kg\/day) of Aronia melanocarpa extract (SAE) in rats with metabolic syndrome (MetS). SAE is an official product of pharmaceutical company Pharmanova (Belgrade, Serbia); however, the procedure for extraction was done by EU-Chem company (Belgrade, Serbia). Rats were divided randomly into six groups: control with Sd, control with Sd and SAE, MetS with HFd, MetS with HFd and SAE, MetS with Sd and MetS with Sd and SAE during 4 weeks. At the end of the 4-week protocol, cardiac function and liver morphology were assessed, while in the blood samples glucose, insulin, iron levels and systemic redox state were determined. Our results demonstrated that SAE had the ability to lower blood pressure and exert benefits on in vivo and ex vivo heart function. Moreover, SAE improved glucose tolerance, attenuated pathological liver alterations and oxidative stress present in MetS. Obtained beneficial effects of SAE were more prominent in combination with changing dietary habits. Promising potential of SAE supplementation alone or in combination with different dietary protocols in triggering cardioprotection should be further examined in future.<\/jats:p>","DOI":"10.3390\/ijms20010006","type":"journal-article","created":{"date-parts":[[2018,12,20]],"date-time":"2018-12-20T12:54:36Z","timestamp":1545310476000},"page":"6","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":39,"title":["Standardized Aronia melanocarpa Extract as Novel Supplement against Metabolic Syndrome: A Rat Model"],"prefix":"10.3390","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0071-8376","authenticated-orcid":false,"given":"Vladimir","family":"JAKOVLJEVIC","sequence":"first","affiliation":[{"name":"Department of Physiology, Faculty of Medical Sciences, University of Kragujevac, Svetozara Markovica 69, 34000 Kragujevac, Serbia"},{"name":"Department of Human Pathology, 1st Moscow State Medical, University IM Sechenov, Trubetskaya street 8, Moscow 119991, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Petar","family":"Milic","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, High Medical School of Professional Studies in Cuprija, Lole Ribara 1\/2, 35000 Cuprija, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jovana","family":"Bradic","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Faculty of Medical Sciences, University of Kragujevac, Svetozara Markovica 69, 34000 Kragujevac, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jovana","family":"Jeremic","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Faculty of Medical Sciences, University of Kragujevac, Svetozara Markovica 69, 34000 Kragujevac, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vladimir","family":"Zivkovic","sequence":"additional","affiliation":[{"name":"Department of Physiology, Faculty of Medical Sciences, University of Kragujevac, Svetozara Markovica 69, 34000 Kragujevac, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ivan","family":"Srejovic","sequence":"additional","affiliation":[{"name":"Department of Physiology, Faculty of Medical Sciences, University of Kragujevac, Svetozara Markovica 69, 34000 Kragujevac, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9105-1350","authenticated-orcid":false,"given":"Tamara","family":"Nikolic Turnic","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Faculty of Medical Sciences, University of Kragujevac, Svetozara Markovica 69, 34000 Kragujevac, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isidora","family":"Milosavljevic","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Faculty of Medical Sciences, University of Kragujevac, Svetozara Markovica 69, 34000 Kragujevac, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nevena","family":"Jeremic","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Faculty of Medical Sciences, University of Kragujevac, Svetozara Markovica 69, 34000 Kragujevac, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergey","family":"Bolevich","sequence":"additional","affiliation":[{"name":"Department of Human Pathology, 1st Moscow State Medical, University IM Sechenov, Trubetskaya street 8, Moscow 119991, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Milica","family":"Labudovic Borovic","sequence":"additional","affiliation":[{"name":"Institute of Histology and Embryology \u201cAleksandar Dj. Kostic\u201d, Faculty of Medicine, University of Belgrade, Dr Subotic 8,  11000 Belgrad, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miroslav","family":"Mitrovic","sequence":"additional","affiliation":[{"name":"Pharmanova, Generala Anrija 6, 11010 Belgrade, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8563-594X","authenticated-orcid":false,"given":"Vesna","family":"Vucic","sequence":"additional","affiliation":[{"name":"Institute for Medical Research, Centre of Research Excellence in Nutrition and Metabolism, University of Belgrade, Tadeusa Koscuska 1, 11129 Belgrade, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2018,12,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1186\/s40200-015-0184-8","article-title":"Targeting metabolic disorders by natural products","volume":"14","author":"Larijani","year":"2015","journal-title":"J. Diabetes Metab. Disord."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"E24","DOI":"10.5888\/pcd14.160287","article-title":"Metabolic Syndrome Prevalence by Race\/Ethnicity and Sex in the United States, National Health and Nutrition Examination Survey, 1988\u20132012","volume":"14","author":"Moore","year":"2017","journal-title":"Prev. Chronic Dis."},{"key":"ref_3","first-page":"4","article-title":"Changes in Antioxidant Enzymes in Metabolic Syndrome Patients after Consumption a Citrus-Based Juice Enriched with Aronia Melanocarpa","volume":"5","author":"Mulero","year":"2015","journal-title":"J. Nutr. Disorders Ther."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"366","DOI":"10.1111\/j.1742-7843.2008.00362.x","article-title":"Protective effects of ethanolic extract of Zingiber officinale rhizome on the development of metabolic syndrome in high-fat diet-fed rats","volume":"104","author":"Nammi","year":"2009","journal-title":"Basic Clin. Pharmacol. Toxicol."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Ranasinghe, P., Mathangasinghe, Y., Jayawardena, R., Hills, A.P., and Misra, A. (2017). Prevalence and trends of metabolic syndrome among adults in the asia-pacific region: A systematic review. BMC Public Health, 17.","DOI":"10.1186\/s12889-017-4041-1"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"De la Iglesia, R., Loria-Kohen, V., Zulet, M.A., Martinez, J.A., Reglero, G., and Ramirez de Molina, A. (2016). Dietary Strategies Implicated in the Prevention and Treatment of Metabolic Syndrome. Int. J. Mol. Sci., 17.","DOI":"10.3390\/ijms17111877"},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Daskalova, E., Delchev, S., Peeva, Y., Vladimirova-Kitova, L., Kratchanova, M., Kratchanov, C., and Denev, P. (2015). Antiatherogenic and Cardioprotective Effects of Black Chokeberry (Aronia melanocarpa) Juice in Aging Rats. Evid. Based Complement Alternat. Med., 2015.","DOI":"10.1155\/2015\/717439"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1089\/jmf.2009.0062","article-title":"Aronia plants: A review of traditional use, biological activities, and perspectives for modern medicine","volume":"13","author":"Kokotkiewicz","year":"2010","journal-title":"J. Med. Food"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"253","DOI":"10.15835\/nbha4017181","article-title":"Comparison of some morphological features, quality and chemical content of four cultivars of chokeberry fruits (Aronia melanocarpa)","volume":"40","author":"Ochmian","year":"2012","journal-title":"Not. Bot. Horti. Agrobot. Cluj Napoca"},{"key":"ref_10","first-page":"171","article-title":"Phenolic Content, Antioxidant Capacity and Quality of Chokeberry (Aronia melanocarpa) Products","volume":"53","year":"2015","journal-title":"Food Technol. Biotechnol."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Cebova, M., Klimentova, J., Janega, P., and Pechanova, O. (2017). Effect of Bioactive Compound of Aronia melanocarpa on Cardiovascular System in Experimental Hypertension. Oxid. Med. Cell. Longev., 2017.","DOI":"10.1155\/2017\/8156594"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Jurikova, T., Mlcek, J., Skrovankova, S., Sumczynski, D., Sochor, J., Hlavacova, I., Snopek, L., and Orsavova, J. (2017). Fruits of Black Chokeberry Aronia melanocarpa in the Prevention of Chronic Diseases. Molecules, 22.","DOI":"10.3390\/molecules22060944"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"115","DOI":"10.26417\/ejis.v4i1.p115-121","article-title":"Research Concerning the Efficiency of Aronia Melanocarpa for Pharmaceutical Purpose","volume":"4","author":"Lupascu","year":"2016","journal-title":"Eur. J. Interdiscip. Stud."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"982","DOI":"10.1111\/1541-4337.12221","article-title":"Chokeberries (Aronia melanocarpa) and Their Products as a Possible Means for the Prevention and Treatment of Noncommunicable Diseases and Unfavorable Health Effects Due to Exposure to Xenobiotics","volume":"15","author":"Borowska","year":"2016","journal-title":"Compr. Rev. Food Sci. Food Saf."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"996","DOI":"10.1016\/j.arr.2013.05.007","article-title":"Should visceral fat be reduced to increase longevity?","volume":"12","author":"Finelli","year":"2013","journal-title":"Ageing Res. Rev."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1189","DOI":"10.1111\/j.1365-2362.2012.02706.x","article-title":"Vitamin D attenuates high fat diet-induced hepatic steatosis in rats by modulating lipid metabolism","volume":"42","author":"Yin","year":"2012","journal-title":"Eur. J. Clin. Investig."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1385\/ENDO:22:2:85","article-title":"The development of diabetes mellitus in Wistar rats kept on a high-fat\/low-carbohydrate diet for long periods","volume":"22","author":"Wang","year":"2003","journal-title":"Endocrine"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1186\/1758-5996-6-130","article-title":"Metabolic and oxidative stress markers in Wistar rats after 2 months on a high-fat diet","volume":"6","author":"Auberval","year":"2014","journal-title":"Diabetol. Metab. Syndr."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1016\/j.jff.2010.04.004","article-title":"Blood pressure-lowering properties of chokeberry (Aronia mitchurinii var. Viking)","volume":"2","author":"Shikov","year":"2010","journal-title":"J. Funct. Foods"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"280","DOI":"10.31989\/ffhd.v7i4.354","article-title":"Reduction of blood pressure by aronia berries through inhibition of angiotensin-converting enzyme activity in the spontaneously hypertensive rat kidney","volume":"7","author":"Yamane","year":"2017","journal-title":"Funct. Food Health Dis."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"493","DOI":"10.4236\/jbise.2013.64062","article-title":"Polyphenolic extract association with renin inhibitors in experimental arterial hypertension","volume":"6","author":"Ciocoiu","year":"2013","journal-title":"J. Biomed. Sci. Eng."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"e179","DOI":"10.1016\/j.atherosclerosis.2006.12.032","article-title":"Combination therapy of statin with flavonoids rich extract from chokeberry fruits enhanced reduction in cardiovascular risk markers in patients after myocardial infraction (MI)","volume":"194","author":"Naruszewicz","year":"2007","journal-title":"Atherosclerosis"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1054","DOI":"10.1017\/S0007114515000562","article-title":"Polyphenol-rich juices reduce blood pressure measures in a randomised controlled trial in high normal and hypertensive volunteers","volume":"114","author":"Tjelle","year":"2015","journal-title":"Br. J. Nutr."},{"key":"ref_24","first-page":"CR28","article-title":"Aronia melanocarpa extract reduces blood pressure, serum endothelin, lipid, and oxidative stress marker levels in patients with metabolic syndrome","volume":"16","author":"Broncel","year":"2010","journal-title":"Med. Sci. Monit."},{"key":"ref_25","first-page":"CC05","article-title":"Protective effects of Ethanolic Extract of Emblica officinalis (amla) on Cardiovascular Pathophysiology of Rats, Fed with High Fat Diet","volume":"11","author":"Kanthe","year":"2017","journal-title":"J. Clin. Diagn. Res."},{"key":"ref_26","unstructured":"El-Sayyad, S.M., Makboul, M.A., Ali, R.M., and Farag, S.F. (2016). Hypotensive effect of Ficus sycomorus L. on the arterial blood pressure of rabbits. J. Pharmacogn. Phytochem., 4, Available online: https:\/\/www.researchgate.net\/publication\/309610756_Hypotensive_effect_of_Ficus_sycomorus_L_on_the_arterial_blood_pressure_of_rabbits."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"617","DOI":"10.1016\/j.nut.2010.10.013","article-title":"Polyphenols: Planting the seeds of treatment for the metabolic syndrome","volume":"27","author":"Cherniack","year":"2011","journal-title":"Nutrition"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1796357","DOI":"10.1155\/2016\/1796357","article-title":"Sex-Dependent Effects of Dietary Genistein on Echocardiographic Profile and Cardiac GLUT4 Signaling in Mice","volume":"2016","author":"Leung","year":"2016","journal-title":"Evid. Based Complement Alternat. Med."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"423","DOI":"10.15171\/apb.2016.055","article-title":"Cardioprotective Effect of Grape Seed Extract on Chronic Doxorubicin-Induced Cardiac Toxicity in Wistar Rats","volume":"6","author":"Razmaraii","year":"2016","journal-title":"Adv. Pharm. Bull."},{"key":"ref_30","first-page":"85","article-title":"Echocardiography findings after intravenous injection of Achillea millefolium (Yarrow) extract in the dog","volume":"44","author":"Yadegari","year":"2015","journal-title":"Malays. Appl. Biol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"174","DOI":"10.2174\/1381612822666151112152143","article-title":"Cardioprotective effects of Aronia melanocarpa anthocynanins. From laboratory experiments to clinical practice","volume":"22","author":"Parzonko","year":"2016","journal-title":"Curr. Pharm. Des."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Kim, N.H., Jegal, J., Kim, Y.N., Heo, J.D., Rho, J.R., Yang, M.H., and Jeong, E.J. (2018). Chokeberry Extract and Its Active Polyphenols Suppress Adipogenesis in 3T3-L1 Adipocytes and Modulates Fat Accumulation and Insulin Resistance in Diet-Induced Obese Mice. Nutrients, 10.","DOI":"10.3390\/nu10111734"},{"key":"ref_33","first-page":"20","article-title":"Effects of Aronia melanocarpa juice as part of the dietary regimen in patients with diabetes mellitus","volume":"44","author":"Simeonov","year":"2002","journal-title":"Folia Med."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1016\/j.jnutbio.2016.02.004","article-title":"Improvement of blood glucose levels and obesity in mice given aronia juice by inhibition of dipeptidyl peptidase IV and \u03b1-glucosidase","volume":"31","author":"Yamane","year":"2016","journal-title":"J. Nutr. Biochem."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Imam, M.U., Zhang, S., Ma, J., Wang, H., and Wang, F. (2017). Antioxidants Mediate Both Iron Homeostasis and Oxidative Stress. Nutrients, 9.","DOI":"10.3390\/nu9070671"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1016\/j.phrp.2014.06.005","article-title":"Relationship between Serum Levels of Body Iron Parameters and Insulin Resistance and Metabolic Syndrome in Korean Children","volume":"5","author":"Lee","year":"2014","journal-title":"Osong Public Health Res. Perspect."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1186\/s12970-014-0048-5","article-title":"Effect of supplementation with chokeberry juice on the inflammatory status and markers of iron metabolism in rowers","volume":"11","author":"Basta","year":"2014","journal-title":"J. Int. Soc. Sports Nutr."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/j.jnutbio.2015.10.015","article-title":"The dietary flavonoid myricetin regulates iron homeostasis by suppressing hepcidin expression","volume":"30","author":"Mu","year":"2016","journal-title":"J. Nutr. Biochem."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"859","DOI":"10.2174\/1381612822666151209152352","article-title":"The potential role of antioxidants in metabolic syndrome","volume":"22","author":"Pereira","year":"2016","journal-title":"Curr. Pharm. Des."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1785","DOI":"10.1039\/C3FO60588D","article-title":"Blueberry polyphenols prevent cardiomyocyte death by preventing calpain activation and oxidative stress","volume":"5","author":"Louis","year":"2014","journal-title":"Food Funct."},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Mattera, R., Benvenuto, M., Giganti, M.G., Tresoldi, I., Pluchinotta, F.R., Bergante, S., Tettamanti, G., Masuelli, L., Manzari, V., and Modesti, A. (2017). Effects of Polyphenols on Oxidative Stress-Mediated Injury in Cardiomyocytes. Nutrients, 9.","DOI":"10.3390\/nu9050523"},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Brglez Mojzer, E., Knez Hrn\u010di\u010d, M., \u0160kerget, M., Knez, \u017d., and Bren, U. (2016). Polyphenols: Extraction Methods, Antioxidative Action, Bioavailability and Anticarcinogenic Effects. Molecules, 21.","DOI":"10.3390\/molecules21070901"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"3490","DOI":"10.1210\/jc.2007-0482","article-title":"Liver fat in the metabolic syndrome","volume":"92","author":"Kotronen","year":"2007","journal-title":"J. Clin. Endocrinol. Metab."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1016\/j.nut.2016.12.009","article-title":"Anthocyanins in chokeberry and purple maize attenuate diet-induced metabolic syndrome in rats","volume":"41","author":"Bhaswant","year":"2017","journal-title":"Nutrition"},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Park, C.H., Kim, J.H., Lee, E.B., Hur, W., Kwon, O.J., Park, H.J., and Yoon, S.K. (2017). Aronia melanocarpa extract ameliorates hepatic lipid metabolism through PPAR\u03b32 downregulation. PLoS ONE, 12.","DOI":"10.1371\/journal.pone.0169685"},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Duchnowicz, P., Ziobro, A., Rapacka, E., Koter-Michalak, M., and Bukowska, B. (2018). Changes in Cholinesterase Activity in Blood of Adolescent with Metabolic Syndrome after Supplementation with Extract from Aronia melanocarpa. BioMed Res. Int.","DOI":"10.1155\/2018\/5670145"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"343","DOI":"10.4149\/gpb_2016055","article-title":"Modulatory effects of naringin on hepatic key enzymes of carbohydrate metabolism in high-fat diet\/low-dose streptozotocin-induced diabetes in rats","volume":"36","author":"Pari","year":"2017","journal-title":"Gen Physiol Biophys"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1288","DOI":"10.1038\/ajh.2008.301","article-title":"Validation of volume-pressure recording tail-cuff blood pressure measurements","volume":"21","author":"Feng","year":"2008","journal-title":"Am. J. Hypertens."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"2111","DOI":"10.1016\/S0735-1097(99)00436-2","article-title":"Abnormal cardiac function in the streptozotocin-induced non-insulin-dependent diabetic rat: Noninvasive assessment with Doppler echocardiography and contribution of the nitric oxide pathway","volume":"34","author":"Joffe","year":"1999","journal-title":"J. Am. Coll. Cardiol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1007\/s11010-012-1398-4","article-title":"The effects of homocysteine-related compounds on cardiac contractility, coronary flow, and oxidative stress markers in isolated rat heart","volume":"370","author":"Zivkovic","year":"2012","journal-title":"Mol. Cell. Biochem."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1016\/0003-2697(82)90118-X","article-title":"Analysis of nitrate, nitrite, and [15N]nitrate in biological fluids","volume":"126","author":"Green","year":"1982","journal-title":"Anal. Biochem."},{"key":"ref_52","unstructured":"Greenvvald, R.A. (1985). Nitroblue tetrazolium reduction. Handbook of Methods for Oxygen Radical Research, CRC Press."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1016\/0022-1759(80)90340-3","article-title":"A simple colorimetric method for the measurement of hydrogen peroxide produced by cells in culture","volume":"38","author":"Pick","year":"1980","journal-title":"J. Immunol. Methods."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"6056","DOI":"10.1016\/S0021-9258(18)63505-7","article-title":"The utility of superoxide dismutase in studying free radical reactions. I. Radicals generated by the interaction of sulfite, dimethyl sulfoxide, and oxygen","volume":"244","author":"McCord","year":"1969","journal-title":"J. Biol. Chem."},{"key":"ref_55","unstructured":"Beutler, E. (1982). Catalase. Red Cell Metabolism, a Manual of Biochemical Methods, Grune and Stratton."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"3170","DOI":"10.1016\/S0021-9258(19)45228-9","article-title":"The role of superoxide-anion in the autooxidation of epinephrine and a simple assay for superoxide dismutase","volume":"247","author":"Misra","year":"1972","journal-title":"J. Biol. Chem."},{"key":"ref_57","unstructured":"Beutler, E. (1975). Reduced glutathione (GSH). Red Cell Metabolism, a Manual of Biochemical Methods, Grune and Stratton."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/20\/1\/6\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T15:35:11Z","timestamp":1760196911000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/20\/1\/6"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,12,20]]},"references-count":57,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2019,1]]}},"alternative-id":["ijms20010006"],"URL":"https:\/\/doi.org\/10.3390\/ijms20010006","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,12,20]]}}}