{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T09:35:52Z","timestamp":1789464952727,"version":"build-2803163510"},"reference-count":44,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2019,2,12]],"date-time":"2019-02-12T00:00:00Z","timestamp":1549929600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["16H04705, 17K07182"],"award-info":[{"award-number":["16H04705, 17K07182"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>TSG101 (Tumor susceptibility 101) gene and its aberrantly spliced isoform, termed TSG101\u2206154-1054, are tightly linked to tumorigenesis in various cancers. The aberrant TSG101\u2206154-1054 mRNA is generated from cancer-specific re-splicing of mature TSG101 mRNA. The TSG101\u2206154-1054 protein protects the full-length TSG101 protein from ubiquitin-mediated degradation, implicating TSG101\u2206154-1054 protein in the progression of cancer. Here, we confirmed that the presence of TSG101\u2206154-1054 mRNA indeed caused an accumulation of the TSG101 protein in biopsies of human nasopharyngeal carcinoma (NPC), which was recapitulated by the overexpression of TSG101\u2206154-1054 in the NPC cell line TW01. We demonstrate the potential function of the TSG101\u2206154-1054 protein in the malignancy of human NPC with scratch-wound healing and transwell invasion assays. By increasing the stability of the TSG101 protein, TSG101\u2206154-1054 specifically enhanced TSG101-mediated TW01 cell migration and invasion, suggesting the involvement in NPC metastasis in vivo. This finding sheds light on the functional significance of TSG101\u2206154-1054 generation via re-splicing of TSG101 mRNA in NPC metastasis and hints at its potential importance as a therapeutic target.<\/jats:p>","DOI":"10.3390\/ijms20030773","type":"journal-article","created":{"date-parts":[[2019,2,13]],"date-time":"2019-02-13T02:49:44Z","timestamp":1550026184000},"page":"773","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Cancer-Specifically Re-Spliced TSG101 mRNA Promotes Invasion and Metastasis of Nasopharyngeal Carcinoma"],"prefix":"10.3390","volume":"20","author":[{"given":"Huey-Huey","family":"Chua","sequence":"first","affiliation":[{"name":"Department of Pediatrics, National Taiwan University Hospital, College of Medicine, National Taiwan University, Taipei 10051, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9562-550X","authenticated-orcid":false,"given":"Toshiki","family":"Kameyama","sequence":"additional","affiliation":[{"name":"Division of Gene Expression Mechanism, Institute for Comprehensive Medical Science, Fujita Health University, Kutsukake-cho, Toyoake, Aichi 470-1192, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9562-550X","authenticated-orcid":false,"given":"Akila","family":"Mayeda","sequence":"additional","affiliation":[{"name":"Division of Gene Expression Mechanism, Institute for Comprehensive Medical Science, Fujita Health University, Kutsukake-cho, Toyoake, Aichi 470-1192, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3818-2818","authenticated-orcid":false,"given":"Te-Huei","family":"Yeh","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology, National Taiwan University Hospital, College of Medicine, National Taiwan University, Taipei 10051, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2019,2,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1293","DOI":"10.1111\/j.1349-7006.2008.00825.x","article-title":"Endosomal sorting complex required for transport proteins in cancer pathogenesis, vesicular transport, and non-endosomal functions","volume":"99","author":"Tanaka","year":"2008","journal-title":"Cancer Sci."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"613","DOI":"10.1042\/BST20160479","article-title":"Growing functions of the ESCRT machinery in cell biology and viral replication","volume":"45","author":"Scourfield","year":"2017","journal-title":"Biochem. 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