{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T22:43:17Z","timestamp":1790462597733,"version":"4.1.0"},"reference-count":56,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2019,3,19]],"date-time":"2019-03-19T00:00:00Z","timestamp":1552953600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001665","name":"French National Research Agency","doi-asserted-by":"publisher","award":["ANR-10-LABX -53-01)."],"award-info":[{"award-number":["ANR-10-LABX -53-01)."]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Monoclonal antibodies (mAbs) are promising therapies to treat airway chronic inflammatory disease (asthma or nasal polyps). To date, no study has specifically assessed, in vitro, the potential function of neonatal Fc receptor (FcRn) in IgG transcytosis through the human nasal airway epithelium. The objective of this study was to report the in vitro expression and function of FcRn in nasal human epithelium. FcRn expression was studied in an air\u2013liquid interface (ALI) primary culture model of human nasal epithelial cells (HNEC) from polyps. FcRn expression was characterized by quantitative RT-PCR, western blot, and immunolabeling. The ability of HNECs to support mAb transcytosis via FcRn was assessed by transcytosis assay. This study demonstrates the expression of FcRn mRNA and protein in HNEC. We report a high expression of FcRn in the cytosol of ciliated, mucus, and basal cells by immunohistochemistry with a higher level of FcRn proteins in differentiated HNEC. We also proved in vitro transepithelial delivery of an IgG1 therapeutic mAb with a dose\u2013response curve. This is the first time that FcRn expression and mAb transcytosis has been shown in a model of human nasal respiratory epithelium in vitro. This study is a prerequisite for FcRn-dependent nasal administration of mAbs.<\/jats:p>","DOI":"10.3390\/ijms20061379","type":"journal-article","created":{"date-parts":[[2019,3,19]],"date-time":"2019-03-19T12:12:25Z","timestamp":1552997545000},"page":"1379","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["FcRn-Dependent Transcytosis of Monoclonal Antibody in Human Nasal Epithelial Cells In Vitro: A Prerequisite for a New Delivery Route for Therapy?"],"prefix":"10.3390","volume":"20","author":[{"given":"Emilie","family":"Bequignon","sequence":"first","affiliation":[{"name":"AP-HP, H\u00f4pital Henri Mondor et Centre Hospitalier Intercommunal de Cr\u00e9teil, service d\u2019Oto-Rhino-Laryngologie et de Chirurgie cervico-faciale, 94010 Cr\u00e9teil, France"},{"name":"INSERM, U955, 94010 Cr\u00e9teil, France"},{"name":"Universit\u00e9 Paris-Est, Facult\u00e9 de M\u00e9decine, F-94010 Cr\u00e9teil, France"},{"name":"CNRS, ERL 7240, 94010 Cr\u00e9teil, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine","family":"Dhomm\u00e9e","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Tours, EA 7501 GICC, F-37032 Tours, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christelle","family":"Angely","sequence":"additional","affiliation":[{"name":"INSERM, U955, 94010 Cr\u00e9teil, France"},{"name":"Universit\u00e9 Paris-Est, Facult\u00e9 de M\u00e9decine, F-94010 Cr\u00e9teil, France"},{"name":"CNRS, ERL 7240, 94010 Cr\u00e9teil, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucie","family":"Thomas","sequence":"additional","affiliation":[{"name":"Inserm U933, 75012 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mathieu","family":"Bottier","sequence":"additional","affiliation":[{"name":"INSERM, U955, 94010 Cr\u00e9teil, France"},{"name":"Universit\u00e9 Paris-Est, Facult\u00e9 de M\u00e9decine, F-94010 Cr\u00e9teil, France"},{"name":"CNRS, ERL 7240, 94010 Cr\u00e9teil, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Estelle","family":"Escudier","sequence":"additional","affiliation":[{"name":"Inserm U933, 75012 Paris, France"},{"name":"Universit\u00e9 Pierre et Marie Curie, Facult\u00e9 de M\u00e9decine, 75005 Paris, France"},{"name":"AP-HP H\u00f4pital Armand-Trousseau, Service de g\u00e9n\u00e9tique et d\u2019embryologie m\u00e9dicale, 75012 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Isabey","sequence":"additional","affiliation":[{"name":"INSERM, U955, 94010 Cr\u00e9teil, France"},{"name":"Universit\u00e9 Paris-Est, Facult\u00e9 de M\u00e9decine, F-94010 Cr\u00e9teil, France"},{"name":"CNRS, ERL 7240, 94010 Cr\u00e9teil, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andr\u00e9","family":"Coste","sequence":"additional","affiliation":[{"name":"AP-HP, H\u00f4pital Henri Mondor et Centre Hospitalier Intercommunal de Cr\u00e9teil, service d\u2019Oto-Rhino-Laryngologie et de Chirurgie cervico-faciale, 94010 Cr\u00e9teil, France"},{"name":"INSERM, U955, 94010 Cr\u00e9teil, France"},{"name":"Universit\u00e9 Paris-Est, Facult\u00e9 de M\u00e9decine, F-94010 Cr\u00e9teil, France"},{"name":"CNRS, ERL 7240, 94010 Cr\u00e9teil, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruno","family":"Louis","sequence":"additional","affiliation":[{"name":"INSERM, U955, 94010 Cr\u00e9teil, France"},{"name":"Universit\u00e9 Paris-Est, Facult\u00e9 de M\u00e9decine, F-94010 Cr\u00e9teil, France"},{"name":"CNRS, ERL 7240, 94010 Cr\u00e9teil, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Fran\u00e7ois","family":"Papon","sequence":"additional","affiliation":[{"name":"INSERM, U955, 94010 Cr\u00e9teil, France"},{"name":"CNRS, ERL 7240, 94010 Cr\u00e9teil, France"},{"name":"AP-HP, H\u00f4pital Bic\u00eatre, Service d\u2019Oto-Rhino-Laryngologie et de Chirurgie cervico-faciale, 94270 Le Kremlin-Bic\u00eatre, France"},{"name":"Facult\u00e9 de M\u00e9decine, Universit\u00e9 Paris-Sud, F-94275 Le Kremlin-Bic\u00eatre, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Val\u00e9rie","family":"Gouilleux-Gruart","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Tours, EA 7501 GICC, F-37032 Tours, France"},{"name":"CHRU de TOURS, Laboratoire d\u2019Immunologie, F-37032 Tours, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2019,3,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1352","DOI":"10.1038\/2031352a0","article-title":"A Theoretical Model of Gamma-Globulin Catabolism","volume":"203","author":"Brambell","year":"1964","journal-title":"Nature"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1038\/337184a0","article-title":"An Fc receptor structurally related to MHC class I antigens","volume":"337","author":"Simister","year":"1989","journal-title":"Nature"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1369\/0022155417705095","article-title":"Distribution of FcRn Across Species and Tissues","volume":"65","author":"Latvala","year":"2017","journal-title":"J. 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