{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T01:15:47Z","timestamp":1790817347479,"version":"4.1.0"},"reference-count":63,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2019,3,20]],"date-time":"2019-03-20T00:00:00Z","timestamp":1553040000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["SFB900"],"award-info":[{"award-number":["SFB900"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Viral infections and reactivations remain a serious obstacle to successful hematopoietic stem cell transplantation (HSCT). When antiviral drug treatment fails, adoptive virus-specific T-cell transfer provides an effective alternative. Assuming that naive T cells (TN) are mainly responsible for GvHD, methods were developed to generate naive T-cell-depleted products while preserving immune memory against viral infections. We compared two major strategies to deplete potentially alloreactive T cells: CD45RA and CD62L depletion and analyzed phenotype and functionality of the resulting CD45RA\u2212\/CD62L\u2212 naive T-cell-depleted as well as CD45RA+\/CD62L+ naive T-cell-enriched fractions in the CMV pp65 and IE1 antigen model. CD45RA depletion resulted in loss of terminally differentiated effector memory T cells re-expressing CD45RA (TEMRA), and CD62L depletion in loss of central memory T cells (TCM). Based on these differences in target cell-dependent and target cell-independent assays, antigen-specific T-cell responses in CD62L-depleted fraction were consistently 3\u20135 fold higher than those in CD45RA-depleted fraction. Interestingly, we also observed high donor variability in the CD45RA-depleted fraction, resulting in a substantial loss of immune memory. Accordingly, we identified donors with expected response (DER) and unexpected response (DUR). Taken together, our results showed that a naive T-cell depletion method should be chosen individually, based on the immunophenotypic composition of the T-cell populations present.<\/jats:p>","DOI":"10.3390\/ijms20061415","type":"journal-article","created":{"date-parts":[[2019,3,21]],"date-time":"2019-03-21T04:11:56Z","timestamp":1553141516000},"page":"1415","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Robust Identification of Suitable T-Cell Subsets for Personalized CMV-Specific T-Cell Immunotherapy Using CD45RA and CD62L Microbeads"],"prefix":"10.3390","volume":"20","author":[{"given":"Caroline","family":"Mangare","sequence":"first","affiliation":[{"name":"Institute for Transfusion Medicine, Hannover Medical School, 30625 Hannover, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabine","family":"Tischer-Zimmermann","sequence":"additional","affiliation":[{"name":"Institute for Transfusion Medicine, Hannover Medical School, 30625 Hannover, Germany"},{"name":"Integrated Research and Treatment Center (IFB-Tx), Hannover Medical School, 30625 Hannover, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sebastian B.","family":"Riese","sequence":"additional","affiliation":[{"name":"Institute for Transfusion Medicine, Hannover Medical School, 30625 Hannover, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5228-7627","authenticated-orcid":false,"given":"Anna C.","family":"Dragon","sequence":"additional","affiliation":[{"name":"Institute for Transfusion Medicine, Hannover Medical School, 30625 Hannover, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Immo","family":"Prinz","sequence":"additional","affiliation":[{"name":"Institute of Immunology, Hannover Medical School, 30625 Hannover, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rainer","family":"Blasczyk","sequence":"additional","affiliation":[{"name":"Institute for Transfusion Medicine, Hannover Medical School, 30625 Hannover, Germany"},{"name":"Integrated Research and Treatment Center (IFB-Tx), Hannover Medical School, 30625 Hannover, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Britta","family":"Maecker-Kolhoff","sequence":"additional","affiliation":[{"name":"Integrated Research and Treatment Center (IFB-Tx), Hannover Medical School, 30625 Hannover, Germany"},{"name":"Department of Pediatric Hematology and Oncology, Hannover Medical School, 30625 Hannover, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Britta","family":"Eiz-Vesper","sequence":"additional","affiliation":[{"name":"Institute for Transfusion Medicine, Hannover Medical School, 30625 Hannover, Germany"},{"name":"Integrated Research and Treatment Center (IFB-Tx), Hannover Medical School, 30625 Hannover, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2019,3,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"242ra83","DOI":"10.1126\/scitranslmed.3008825","article-title":"Activity of broad-spectrum T cells as treatment for AdV, EBV, CMV, BKV, and HHV6 infections after HSCT","volume":"6","author":"Papadopoulou","year":"2014","journal-title":"Sci. Transl. Med."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"2483","DOI":"10.1038\/s41375-018-0123-z","article-title":"Baricitinib-induced blockade of interferon gamma receptor and interleukin-6 receptor for the prevention and treatment of graft-versus-host disease","volume":"32","author":"Choi","year":"2018","journal-title":"Leukemia"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"780","DOI":"10.1053\/j.ajkd.2014.11.020","article-title":"Brincidofovir for polyomavirus-associated nephropathy after allogeneic hematopoietic stem cell transplantation","volume":"65","author":"Papanicolaou","year":"2015","journal-title":"Am. J. Kidney Dis."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"2159","DOI":"10.1182\/bloodadvances.2018016493","article-title":"Antiviral prophylaxis for cytomegalovirus infection in allogeneic hematopoietic cell transplantation","volume":"2","author":"Chen","year":"2018","journal-title":"Blood Adv."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"585","DOI":"10.1080\/17474086.2016.1174571","article-title":"Treatment of CMV infection after allogeneic hematopoietic stem cell transplantation","volume":"9","author":"Maffini","year":"2016","journal-title":"Expert Rev. Hematol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"e00922-18","DOI":"10.1128\/AAC.00922-18","article-title":"New Locus of Drug Resistance in the Human Cytomegalovirus UL56 Gene Revealed by In Vitro Exposure to Letermovir and Ganciclovir","volume":"62","author":"Chou","year":"2018","journal-title":"Antimicrob Agents Chemother."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"10884","DOI":"10.1128\/JVI.05265-11","article-title":"The novel anticytomegalovirus compound AIC246 (Letermovir) inhibits human cytomegalovirus replication through a specific antiviral mechanism that involves the viral terminase","volume":"85","author":"Goldner","year":"2011","journal-title":"J. Virol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"3547","DOI":"10.1200\/JCO.2017.73.0655","article-title":"Off-the-Shelf Virus-Specific T Cells to Treat BK Virus, Human Herpesvirus 6, Cytomegalovirus, Epstein-Barr Virus, and Adenovirus Infections After Allogeneic Hematopoietic Stem-Cell Transplantation","volume":"35","author":"Tzannou","year":"2017","journal-title":"J. Clin. Oncol"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"342","DOI":"10.1182\/asheducation-2013.1.342","article-title":"T-cell therapy for viral infections","volume":"2013","author":"Heslop","year":"2013","journal-title":"Hematol. Am. Soc. Hematol. Educ. Program."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1016\/j.beha.2008.02.007","article-title":"Graft-versus-host disease after donor leukocyte infusions: Presentation and management","volume":"21","author":"Frey","year":"2008","journal-title":"Best Pract Res. Clin. Haematol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1038\/sj.bmt.1702726","article-title":"Prophylactic donor lymphocyte infusions after moderately ablative chemotherapy and stem cell transplantation for hematological malignancies: High remission rate among poor prognosis patients at the expense of graft-versus-host disease","volume":"27","author":"Bonamino","year":"2001","journal-title":"Bone Marrow Transplant."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"4360","DOI":"10.1182\/blood-2010-01-262089","article-title":"Adoptive transfer of pp65-specific T cells for the treatment of chemorefractory cytomegalovirus disease or reactivation after haploidentical and matched unrelated stem cell transplantation","volume":"116","author":"Feuchtinger","year":"2010","journal-title":"Blood"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"336","DOI":"10.1186\/s12967-014-0336-5","article-title":"Rapid generation of clinical-grade antiviral T cells: Selection of suitable T-cell donors and GMP-compliant manufacturing of antiviral T cells","volume":"12","author":"Tischer","year":"2014","journal-title":"J. Transl. Med."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"393","DOI":"10.3389\/fimmu.2016.00393","article-title":"Comparative Analysis of Clinical-Scale IFN-gamma-Positive T-Cell Enrichment Using Partially and Fully Integrated Platforms","volume":"7","author":"Priesner","year":"2016","journal-title":"Front. Immunol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1007\/s00262-009-0778-4","article-title":"Multimer technologies for detection and adoptive transfer of antigen-specific T cells","volume":"59","author":"Schmitt","year":"2010","journal-title":"Cancer Immunol. Immunother."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"631","DOI":"10.1038\/nm0602-631","article-title":"Reversible MHC multimer staining for functional isolation of T-cell populations and effective adoptive transfer","volume":"8","author":"Knabel","year":"2002","journal-title":"Nat. Med."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"2161","DOI":"10.1038\/leu.2017.16","article-title":"Transfer of minimally manipulated CMV-specific T cells from stem cell or third-party donors to treat CMV infection after allo-HSCT","volume":"31","author":"Neuenhahn","year":"2017","journal-title":"Leukemia"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Gerdemann, U., Vera, J.F., Rooney, C.M., and Leen, A.M. (2011). Generation of multivirus-specific T cells to prevent\/treat viral infections after allogeneic hematopoietic stem cell transplant. J. Vis. Exp.","DOI":"10.3791\/2736-v"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1097\/CJI.0000000000000034","article-title":"First-in-man clinical results with good manufacturing practice (GMP)-compliant polypeptide-expanded adenovirus-specific T cells after haploidentical hematopoietic stem cell transplantation","volume":"37","author":"Geyeregger","year":"2014","journal-title":"J. Immunother."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1016\/j.jcyt.2013.11.010","article-title":"The time is now: Moving toward virus-specific T cells after allogeneic hematopoietic stem cell transplantation as the standard of care","volume":"16","author":"Saglio","year":"2014","journal-title":"Cytotherapy"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"939","DOI":"10.1080\/21505594.2016.1207038","article-title":"Immunotherapy for opportunistic infections: Current status and future perspectives","volume":"7","author":"Fuji","year":"2016","journal-title":"Virulence"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1016\/j.beha.2011.06.003","article-title":"Adoptive immunotherapy with virus-specific T cells","volume":"24","author":"Fuji","year":"2011","journal-title":"Best Pract. Res. Clin. Haematol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1351","DOI":"10.1038\/s41409-018-0209-2","article-title":"Adoptive transfer of cellular immunity against cytomegalovirus by virus-specific lymphocytes from a third-party family donor","volume":"53","author":"Lindemann","year":"2018","journal-title":"Bone Marrow Transplant."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"8649","DOI":"10.1128\/JVI.75.18.8649-8659.2001","article-title":"Differential immunogenicity of Epstein-Barr virus latent-cycle proteins for human CD4(+) T-helper 1 responses","volume":"75","author":"Leen","year":"2001","journal-title":"J. Virol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1024","DOI":"10.3324\/haematol.2010.037481","article-title":"Alloreactive and leukemia-reactive T cells are preferentially derived from naive precursors in healthy donors: Implications for immunotherapy with memory T cells","volume":"96","author":"Distler","year":"2011","journal-title":"Haematologica"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"e12823","DOI":"10.1111\/tid.12823","article-title":"Selective T-cell depletion targeting CD45RA reduces viremia and enhances early T-cell recovery compared with CD3-targeted T-cell depletion","volume":"20","author":"Triplett","year":"2018","journal-title":"Transpl. Infect. Dis."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"705","DOI":"10.1016\/j.bbmt.2014.01.032","article-title":"Engineering human peripheral blood stem cell grafts that are depleted of naive T cells and retain functional pathogen-specific memory T cells","volume":"20","author":"Bleakley","year":"2014","journal-title":"Biol. Blood Marrow Transplant."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1303","DOI":"10.1016\/j.jaci.2014.08.019","article-title":"CD45RA depletion in HLA-mismatched allogeneic hematopoietic stem cell transplantation for primary combined immunodeficiency: A preliminary study","volume":"135","author":"Touzot","year":"2015","journal-title":"J. Allergy Clin. Immunol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"5726132","DOI":"10.1155\/2016\/5726132","article-title":"Review on Haploidentical Hematopoietic Cell Transplantation in Patients with Hematologic Malignancies","volume":"2016","author":"Fabricius","year":"2016","journal-title":"Adv. Hematol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"138","DOI":"10.1038\/bmt.2013.114","article-title":"Depletion of naive T cells using clinical grade magnetic CD45RA beads: A new approach for GVHD prophylaxis","volume":"49","author":"Teschner","year":"2014","journal-title":"Bone Marrow Transplant."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1358","DOI":"10.1038\/bmt.2015.135","article-title":"Generation of memory T cells for adoptive transfer using clinical-grade anti-CD62L magnetic beads","volume":"50","author":"Verfuerth","year":"2015","journal-title":"Bone Marrow Transplant."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1038\/nri1292","article-title":"The many important facets of T-cell repertoire diversity","volume":"4","author":"Slifka","year":"2004","journal-title":"Nat. Rev. Immunol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"e97219","DOI":"10.1172\/jci.insight.97219","article-title":"Unique features and clinical importance of acute alloreactive immune responses","volume":"3","author":"Inman","year":"2018","journal-title":"JCI Insight"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1189","DOI":"10.1093\/intimm\/dxn075","article-title":"Phenotypic classification of human CD4+ T cell subsets and their differentiation","volume":"20","author":"Okada","year":"2008","journal-title":"Int Immunol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"975","DOI":"10.1002\/cyto.a.20643","article-title":"Phenotype and function of human T lymphocyte subsets: Consensus and issues","volume":"73","author":"Appay","year":"2008","journal-title":"Cytometry A"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"2473","DOI":"10.1084\/jem.20070784","article-title":"Superior control of HIV-1 replication by CD8+ T cells is reflected by their avidity, polyfunctionality, and clonal turnover","volume":"204","author":"Almeida","year":"2007","journal-title":"J. Exp. Med."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1999","DOI":"10.1084\/jem.20042408","article-title":"Repertoire, diversity, and differentiation of specific CD8 T cells are associated with immune protection against human cytomegalovirus disease","volume":"201","author":"Sacre","year":"2005","journal-title":"J. Exp. Med."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1038\/nm0402-379","article-title":"Memory CD8+ T cells vary in differentiation phenotype in different persistent virus infections","volume":"8","author":"Appay","year":"2002","journal-title":"Nat. Med."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"2797","DOI":"10.1002\/eji.201343751","article-title":"The who\u2019s who of T-cell differentiation: Human memory T-cell subsets","volume":"43","author":"Mahnke","year":"2013","journal-title":"Eur. J. Immunol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"282","DOI":"10.1016\/j.exphem.2003.12.009","article-title":"Isolation and expansion of human adenovirus-specific CD4+ and CD8+ T cells according to IFN-gamma secretion for adjuvant immunotherapy","volume":"32","author":"Feuchtinger","year":"2004","journal-title":"Exp. Hematol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1473","DOI":"10.1038\/s41467-017-01728-5","article-title":"Unique phenotypes and clonal expansions of human CD4 effector memory T cells re-expressing CD45RA","volume":"8","author":"Tian","year":"2017","journal-title":"Nat. Commun."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1128\/CMR.00034-08","article-title":"Immunobiology of human cytomegalovirus: From bench to bedside","volume":"22","author":"Crough","year":"2009","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1016\/j.hoc.2010.11.011","article-title":"Cytomegalovirus in hematopoietic stem cell transplant recipients","volume":"25","author":"Ljungman","year":"2011","journal-title":"Hematol. Oncol. Clin. N. Am."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"432","DOI":"10.1016\/j.humimm.2004.02.022","article-title":"Clinical aspects of CMV infection after stem cell transplantation","volume":"65","author":"Hebart","year":"2004","journal-title":"Hum. Immunol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"2427","DOI":"10.1182\/blood-2015-11-679639","article-title":"Early cytomegalovirus reactivation remains associated with increased transplant-related mortality in the current era: A CIBMTR analysis","volume":"127","author":"Teira","year":"2016","journal-title":"Blood"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"352","DOI":"10.3389\/fimmu.2016.00352","article-title":"CMV-Specific CD8 T Cell Differentiation and Localization: Implications for Adoptive Therapies","volume":"7","author":"Smith","year":"2016","journal-title":"Front. Immunol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"105","DOI":"10.3389\/fimmu.2017.00105","article-title":"\u03b3\u03b4 T Cell-Mediated Immunity to Cytomegalovirus Infection","volume":"8","author":"Khairallah","year":"2017","journal-title":"Front. Immunol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1016\/j.jaut.2016.11.001","article-title":"CD4(+) virtual memory: Antigen-inexperienced T cells reside in the naive, regulatory, and memory T cell compartments at similar frequencies, implications for autoimmunity","volume":"77","author":"Marusina","year":"2017","journal-title":"J. Autoimmun."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"163","DOI":"10.3389\/fimmu.2013.00163","article-title":"The role of regulatory T cells in the biology of graft versus host disease","volume":"4","author":"Beres","year":"2013","journal-title":"Front. Immunol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"532","DOI":"10.1016\/j.jcyt.2018.01.006","article-title":"Generation of alloreactivity-reduced donor lymphocyte products retaining memory function by fully automatic depletion of CD45RA-positive cells","volume":"20","author":"Muller","year":"2018","journal-title":"Cytotherapy"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"2665","DOI":"10.4049\/jimmunol.1101165","article-title":"Functional exhaustion of CD4+ T lymphocytes during primary cytomegalovirus infection","volume":"189","author":"Antoine","year":"2012","journal-title":"J. Immunol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1038\/nri.2016.38","article-title":"T cell responses to cytomegalovirus","volume":"16","author":"Klenerman","year":"2016","journal-title":"Nat. Rev. Immunol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"1984","DOI":"10.4049\/jimmunol.169.4.1984","article-title":"Cytomegalovirus seropositivity drives the CD8 T cell repertoire toward greater clonality in healthy elderly individuals","volume":"169","author":"Khan","year":"2002","journal-title":"J. Immunol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"8218","DOI":"10.4049\/jimmunol.175.12.8218","article-title":"Cytomegalovirus-specific CD4+ T cells in healthy carriers are continuously driven to replicative exhaustion","volume":"175","author":"Fletcher","year":"2005","journal-title":"J. Immunol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"994","DOI":"10.1016\/j.bbmt.2010.02.007","article-title":"Protective immunity transferred by infusion of cytomegalovirus-specific CD8(+) T cells within donor grafts: Its associations with cytomegalovirus reactivation following unmanipulated allogeneic hematopoietic stem cell transplantation","volume":"16","author":"Luo","year":"2010","journal-title":"Biol Blood Marrow Transplant."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1111\/j.1365-2567.2010.03334.x","article-title":"Human cytomegalovirus-specific CD8(+) T-cell expansions contain long-lived cells that retain functional capacity in both young and elderly subjects","volume":"132","author":"Wallace","year":"2011","journal-title":"Immunology"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"5071","DOI":"10.1182\/blood-2009-04-214684","article-title":"The transfer of adaptive immunity to CMV during hematopoietic stem cell transplantation is dependent on the specificity and phenotype of CMV-specific T cells in the donor","volume":"114","author":"Scheinberg","year":"2009","journal-title":"Blood"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"4360","DOI":"10.1182\/blood-2007-07-104604","article-title":"Differential costimulation through CD137 (4\u20131BB) restores proliferation of human virus-specific \u201ceffector memory\u201d (CD28\u2212 CD45RAHI) CD8+ T cells","volume":"110","author":"Waller","year":"2007","journal-title":"Blood"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"5938","DOI":"10.4049\/jimmunol.0802212","article-title":"Central memory CD8+ T cells induce graft-versus-host disease and mediate graft-versus-leukemia","volume":"182","author":"Zheng","year":"2009","journal-title":"J. Immunol."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"1888","DOI":"10.1182\/blood-2010-10-310599","article-title":"Engraftment of human central memory-derived effector CD8+ T cells in immunodeficient mice","volume":"117","author":"Wang","year":"2011","journal-title":"Blood"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"6241","DOI":"10.4049\/jimmunol.1301148","article-title":"Differences in the phenotype, cytokine gene expression profiles, and in vivo alloreactivity of T cells mobilized with plerixafor compared with G-CSF","volume":"191","author":"Lundqvist","year":"2013","journal-title":"J. Immunol."},{"key":"ref_62","doi-asserted-by":"crossref","unstructured":"Bunse, C.E., Borchers, S., Varanasi, P.R., Tischer, S., Figueiredo, C., Immenschuh, S., Kalinke, U., Kohl, U., Goudeva, L., and Maecker-Kolhoff, B. (2013). Impaired functionality of antiviral T cells in G-CSF mobilized stem cell donors: Implications for the selection of CTL donor. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0077925"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"1480","DOI":"10.1016\/j.bbmt.2013.07.015","article-title":"CMV-, EBV- and ADV-specific T cell immunity: Screening and monitoring of potential third-party donors to improve post-transplantation outcome","volume":"19","author":"Sukdolak","year":"2013","journal-title":"Biol Blood Marrow Transplant."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/20\/6\/1415\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T12:39:26Z","timestamp":1760186366000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/20\/6\/1415"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,3,20]]},"references-count":63,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2019,3]]}},"alternative-id":["ijms20061415"],"URL":"https:\/\/doi.org\/10.3390\/ijms20061415","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,3,20]]}}}