{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T22:12:22Z","timestamp":1790201542803,"version":"4.1.0"},"reference-count":44,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2019,3,25]],"date-time":"2019-03-25T00:00:00Z","timestamp":1553472000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Shenzhen Knowledge and Innovation Basic Research Grant","award":["JCYJ20160122151433832"],"award-info":[{"award-number":["JCYJ20160122151433832"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Carbonic anhydrases (CAs) represent a group of enzymes that catalyse important reactions of carbon dioxide hydration and dehydration, a reaction crucial to many biological processes and environmental biotechnology. In this study we successfully constructed a thermostable fusion enzyme composed of the Sulfurihydrogenibium azorense carbonic anhydrase (Saz_CA), the fastest CA discovered to date, and the chitin binding domain (ChBD) of chitinase from Bacillus circulans. Introduction of ChBD to the Saz_CA had no major impact on the effect of ions or inhibitors on the enzymatic activity. The fusion protein exhibited no negative effects up to 60 \u00b0C, whilst the fusion partner appears to protect the enzyme from negative effects of magnesium. The prepared biocatalyst appears to be thermally activated at 60 \u00b0C and could be partially purified with heat treatment. Immobilisation attempts on different kinds of chitin-based support results have shown that the fusion enzyme preferentially binds to a cheap, untreated chitin with a large crystallinity index over more processed forms of chitin. It suggests significant potential economic benefits for large-scale deployment of immobilised CA technologies such as CO2 utilisation or mineralisation.<\/jats:p>","DOI":"10.3390\/ijms20061494","type":"journal-article","created":{"date-parts":[[2019,3,27]],"date-time":"2019-03-27T05:03:12Z","timestamp":1553662992000},"page":"1494","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":35,"title":["Accelerated CO2 Hydration with Thermostable Sulfurihydrogenibium azorense Carbonic Anhydrase-Chitin Binding Domain Fusion Protein Immobilised on Chitin Support"],"prefix":"10.3390","volume":"20","author":[{"given":"Juan","family":"Hou","sequence":"first","affiliation":[{"name":"School of Environment and Energy, Peking University Shenzhen Graduate School, Shenzhen 518055, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xingkang","family":"Li","sequence":"additional","affiliation":[{"name":"School of Environment and Energy, Peking University Shenzhen Graduate School, Shenzhen 518055, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michal B.","family":"Kaczmarek","sequence":"additional","affiliation":[{"name":"School of Environment and Energy, Peking University Shenzhen Graduate School, Shenzhen 518055, China"},{"name":"Institute of Technical Biochemistry, Lodz University of Technology, 90-924 Lodz, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pengyu","family":"Chen","sequence":"additional","affiliation":[{"name":"School of Environment and Energy, Peking University Shenzhen Graduate School, Shenzhen 518055, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kai","family":"Li","sequence":"additional","affiliation":[{"name":"School of Environment and Energy, Peking University Shenzhen Graduate School, Shenzhen 518055, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peng","family":"Jin","sequence":"additional","affiliation":[{"name":"School of Environment and Energy, Peking University Shenzhen Graduate School, Shenzhen 518055, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuanmei","family":"Liang","sequence":"additional","affiliation":[{"name":"School of Environment and Energy, Peking University Shenzhen Graduate School, Shenzhen 518055, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8183-8854","authenticated-orcid":false,"given":"Maurycy","family":"Daroch","sequence":"additional","affiliation":[{"name":"School of Environment and Energy, Peking University Shenzhen Graduate School, Shenzhen 518055, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2019,3,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"338","DOI":"10.3390\/min2040338","article-title":"Cyanobacteria as biocatalysts for carbonate mineralization","volume":"2","author":"Kamennaya","year":"2012","journal-title":"Minerals"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1016\/j.micron.2009.11.012","article-title":"On carbon dioxide storage based on biomineralization strategies","volume":"41","author":"Lee","year":"2010","journal-title":"Micron"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1016\/j.procbio.2018.08.017","article-title":"Efficient carbon dioxide sequestration by using recombinant carbonic anhydrase","volume":"73","author":"Tan","year":"2018","journal-title":"Process Biochem."},{"key":"ref_4","first-page":"65","article-title":"The kinetics of the carbon dioxide-carbonic acid reaction","volume":"232","author":"Brinkman","year":"1933","journal-title":"Philos. 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