{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T23:54:06Z","timestamp":1788479646644,"version":"build-2803163510"},"reference-count":35,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2019,3,25]],"date-time":"2019-03-25T00:00:00Z","timestamp":1553472000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Korean Ministry of Small and Medium scale Enterprises and Startups","award":["C0502529"],"award-info":[{"award-number":["C0502529"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>In this study, we combined enzymatic hydrolysis and lactic acid fermentation to generate an antihypertensive product. Soybean protein isolates were first hydrolyzed by Prozyme and subsequently fermented with Lactobacillus rhamnosus EBD1. After fermentation, the in vitro angiotensin-converting enzyme (ACE) inhibitory activity of the product (P-SPI) increased from 60.8 \u00b1 2.0% to 88.24 \u00b1 3.2%, while captopril (a positive control) had an inhibitory activity of 94.20 \u00b1 5.4%. Mass spectrometry revealed the presence of three potent and abundant ACE inhibitory peptides, PPNNNPASPSFSSSS, GPKALPII, and IIRCTGC in P-SPI. Hydrolyzing P-SPI with gastrointestinal proteases did not significantly affect its ACE inhibitory ability. Also, oral administration of P-SPI (200 mg\/kg body weight) to spontaneous hypertensive rats (SHRs) for 6 weeks significantly lowered systolic blood pressure (\u221219 \u00b1 4 mm Hg, p &lt; 0.05) and controlled body weight gain relative to control SHRs that were fed with physiological saline. Overall, P-SPI could be used as an antihypertensive functional food.<\/jats:p>","DOI":"10.3390\/ijms20061496","type":"journal-article","created":{"date-parts":[[2019,3,27]],"date-time":"2019-03-27T05:03:12Z","timestamp":1553662992000},"page":"1496","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":72,"title":["Development of a Soy Protein Hydrolysate with an Antihypertensive Effect"],"prefix":"10.3390","volume":"20","author":[{"given":"Eric Banan-Mwine","family":"Daliri","sequence":"first","affiliation":[{"name":"Department of Food Science and Biotechnology, Kangwon National University, Chuncheon 200-701, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fred Kwame","family":"Ofosu","sequence":"additional","affiliation":[{"name":"Department of Food Science and Biotechnology, Kangwon National University, Chuncheon 200-701, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ramachandran","family":"Chelliah","sequence":"additional","affiliation":[{"name":"Department of Food Science and Biotechnology, Kangwon National University, Chuncheon 200-701, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mi Houn","family":"Park","sequence":"additional","affiliation":[{"name":"Erom Company Limited, R&amp;D Center, 111, Toegye Nonggong-ro, Chuncheon-si, Gangwon-do 24427, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jong-Hak","family":"Kim","sequence":"additional","affiliation":[{"name":"Erom Company Limited, R&amp;D Center, 111, Toegye Nonggong-ro, Chuncheon-si, Gangwon-do 24427, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7472-0436","authenticated-orcid":false,"given":"Deog-Hwan","family":"Oh","sequence":"additional","affiliation":[{"name":"Department of Food Science and Biotechnology, Kangwon National University, Chuncheon 200-701, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2019,3,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/S0140-6736(16)31919-5","article-title":"Worldwide trends in blood pressure from 1975 to 2015: A pooled analysis of 1479 population-based measurement studies with 19\u00b71 million participants","volume":"389","author":"Zhou","year":"2017","journal-title":"Lancet"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1016\/j.lwt.2018.03.026","article-title":"Novel angiotensin I-converting enzyme inhibitory peptides from soybean protein isolates fermented by Pediococcus pentosaceus SDL1409","volume":"93","author":"Daliri","year":"2018","journal-title":"LWT"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"2273","DOI":"10.1080\/10408398.2017.1319795","article-title":"Current trends and perspectives of bioactive peptides","volume":"58","author":"Daliri","year":"2018","journal-title":"Crit. 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