{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,14]],"date-time":"2026-02-14T03:31:27Z","timestamp":1771039887319,"version":"3.50.1"},"reference-count":47,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2019,4,1]],"date-time":"2019-04-01T00:00:00Z","timestamp":1554076800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["17K16745"],"award-info":[{"award-number":["17K16745"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Dexmedetomidine (DEX), a highly selective alpha2 adrenergic receptor agonist, directly protects hearts against ischemia\/reperfusion (I\/R) injury. However, the detailed mechanism has not been fully elucidated. We studied differentially expressed mRNAs and miRNAs after DEX administration in rat hearts by comprehensive analysis. Additionally, bioinformatics analysis was applied to explore candidate genes and pathways that might play important roles in DEX-induced cardioprotection. The results of microarray analysis showed that 165 mRNAs and 6 miRNAs were differentially expressed after DEX administration. Through bioinformatics analysis using differentially expressed mRNAs, gene ontology (GO) terms including MAP kinase tyrosine\/serine\/threonine phosphatase activity and pathways including the p53 pathway were significantly enriched in the down-regulated mRNAs. Dusp1 and Atm were associated with the GO term of MAP kinase tyrosine\/serine\/threonine phosphatase activity and the p53 pathway, respectively. On the other hand, no significant pathway was found in the target mRNAs of deregulated miRNAs. The results indicated some possible key genes and pathways that seem to be of significance in DEX-induced cardioprotection, although miRNAs seem to be unlikely to contribute to cardioprotection induced by DEX.<\/jats:p>","DOI":"10.3390\/ijms20071614","type":"journal-article","created":{"date-parts":[[2019,4,2]],"date-time":"2019-04-02T03:21:26Z","timestamp":1554175286000},"page":"1614","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Identification of Candidate Genes and Pathways in Dexmedetomidine-Induced Cardioprotection in the Rat Heart by Bioinformatics Analysis"],"prefix":"10.3390","volume":"20","author":[{"given":"Yusuke","family":"Yoshikawa","sequence":"first","affiliation":[{"name":"Department of Anesthesiology, Sapporo Medical University School of Medicine, South 1, west 16, Chuo-ku, Sapporo, Hokkaido 060-8543, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Naoyuki","family":"Hirata","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology, Sapporo Medical University School of Medicine, South 1, west 16, Chuo-ku, Sapporo, Hokkaido 060-8543, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hirofumi","family":"Terada","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology, Sapporo Medical University School of Medicine, South 1, west 16, Chuo-ku, Sapporo, Hokkaido 060-8543, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasuaki","family":"Sawashita","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology, Sapporo Medical University School of Medicine, South 1, west 16, Chuo-ku, Sapporo, Hokkaido 060-8543, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michiaki","family":"Yamakage","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology, Sapporo Medical University School of Medicine, South 1, west 16, Chuo-ku, Sapporo, Hokkaido 060-8543, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2019,4,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1097\/00000542-199708000-00024","article-title":"Isoflurane mimics ischemic preconditioning via activation of K(ATP) channels: Reduction of myocardial infarct size with an acute memory phase","volume":"87","author":"Kersten","year":"1997","journal-title":"Anesthesiology"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"563","DOI":"10.1093\/bja\/74.5.563","article-title":"ATP sparing effect of isoflurane during ischaemia and reperfusion of the canine heart","volume":"74","author":"Kanaya","year":"1995","journal-title":"Br. 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